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Materials and Methods
3 Methods for Verifying Your DNA Plasmids
Have you ever heard the phrase “measure twice, cut once?” In woodworking, this means making sure you...
5 Key Features of DNA Plasmids Explained
Have you heard of DNA plasmids, sometimes called DNA vectors, but you aren’t quite sure what exactly...
3 Key Differences Between AEBSF and PMSF Protease Inhibitors
AEBSF and PMSF are both irreversible protease inhibitors of serine proteases. With them both inhibit...
4 Important Applications for Protein Purification Using Ion Exchange Chromatography
Ion Exchange Chromatography is routinely sandwiched between affinity and size exclusion steps in an...
Develop the Best Protocol for Protein Purification with Ion Exchange Chromatography (IEX)
Does it ever feel like the hardest part of a project is just getting started on the dang thing? For...
How Detergents are Extremely Useful in Bioscience Experiments
Detergents are versatile chemical tools. But how exactly do they help in experiments? Some detergen...
All About Affinity Purification of Antibodies
Antibodies are amazing! Antibodies from our immune cells help keep us healthy and mount a defense wh...
3 Solubility Tags for Protein Affinity Purification
Let’s imagine that you are trying to express and purify a new protein. You attached a his-tag onto y...
E. coli Homologous Recombination: How It Works and Influences on Cloning
Homologous recombination is the exchange of sequences that occurs between two similar (homologous)...