Articles

What’s the Binding Capacity of Glutathione Agarose

You want to purify Glutathione S-transferase (GST) or a GST-fusion protein. So, you pull your GoldBi...

How Biotinylated Molecules are Purified with Streptavidin Beads

treptavidin is a bacterial protein that binds to the small molecule biotin with extremely high affin...

What Does the DE3 Represent in Competent Cells Like BL21(DE3) and DL39(DE3)?*

Expression systems are important tools in molecular biology, enabling researchers to produce protein...

How to Use Magnetic Agarose Beads to Purify Antibodies: A Step-by-Step Protocol

This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, o...

Regular vs Magnetic Agarose Beads: Key Differences and Best Uses

Have you ever wondered whether you should be using regular agarose beads or magnetic agarose beads f...

The Purpose of Magnetic Agarose Beads

Have you ever seen a cartoon where a huge magnet is used to pull objects into the sky? Usually, the...

Easy Step-by-Step Antibody Purification Protocol with Protein A, Protein G, and Protein L Agarose Beads

In many articles, we’ve conceptually discussed Protein A, G, and L agarose beads and how purifying a...

How to Perform Immunoprecipitation with Protein A, G, or L Agarose Beads

mmunoprecipitation (IP) is a powerful and frequently used technique that utilizes antibodies to isol...

How Much Resin for My Purification When Using Protein A, G or L? Binding Capacity Holds Answers

When affinity purifying a protein, one of the first questions you’ll ask yourself is “how much resin...