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5-Ethylindole is a reagent used for chemical synthesis. It forms a reactant for the creation of IL2 T-cell (ITK) inhibitors and Sodium-dependent glucose co-transporter 2 inhibitors (SGLT2), along with small molecule C5a receptor modulators and the synthesis of antitumor agents.

5-Ethylindole-2-carboxylic acid is a reagent used for chemical synthesis useful for synthesizing auxin analogs and pharmaceuticals.

5-Ethylindole-3-acetic acid is an auxin analog of indole-3-acetic acid and a reagent for chemical synthesis.

GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥5 x 10⁷ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

The Ampicillin (Sodium) EZ Pak™ is the fastest and easiest way to make a set amount of sterile ampicillin (sodium) solution. The kit includes pre-weighed ampicillin (sodium) powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H₂O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio ampicillin (sodium) and the sterile solution is ready for tissue culture, bacterial media, or other appropriate use.

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Transformation efficiency: ≥1 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 10⁸ cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

The Carbenicillin (disodium) EZ Pak™ is the fastest and easiest way to make a set amount of sterile carbenicillin (disodium) solution. The kit includes pre-weighed carbenicillin (disodium) powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H₂O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio carbenicillin (disodium) and the sterile solution is ready for tissue culture, bacterial media, or any number of uses.

The Cefotaxime (Sodium) EZ Pak™ is the fastest and easiest way to make a set amount of sterile cefotaxime (sodium) solution. The kit includes pre-weighed cefotaxime (sodium) powder, a sterile filter and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H₂O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio cefotaxime (sodium) and the sterile solution is ready for tissue culture, bacterial media or any number of uses.

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg²⁺ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.

GoldBio’s DH10B Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. DH10B Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

Product Specifications
Competent cell type: Chemically competent
Derivative of: DH10B™
Species: E. coli
Transformation efficiency: ≥8.2 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s DH10B Electrocompetent E. coli cells are high efficiency cells, ≥2 x 10⁸ cfu/µg plasmid DNA, suitable for a wide variety of applications requiring high transformation efficiencies, such as cloning and subcloning, as well as working with either cDNA or gDNA library construction. DH10B Electrocompetent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine. Here, we present a detailed protocol for electroporation using DH10B Electrocompetent E. coli cells.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Transformation efficiency: ≥2 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s DH10B-Pro™ Electrocompetent E. coli cells are especially designed for the most demanding cloning applications. DH10-Pro™ cells will provide the greatest number of transformants for when your research requires it, including assembling large and multi-DNA fragments, cloning large (≥10 kb up to 350 kb) or difficult construct transformations, working with synthetic bio-applications, and even BAC cloning.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Transformation efficiency:≥1 x 10⁹ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s DH5-alpha Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s DH5-alpha Electrocompetent E. coli are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. Plasmid quality and yields are both enhanced in our DH5-alpha cells due to mutations in both endA1 and recA1.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

DL-ethionine changes behavior of hepatic functions by working as a carcinogenic agent that induces cholangiocarcinogensis in vivo and can be used to induce oxidative stress on the liver to measure antioxidant enzymatic activity. In the pancreas it destroys acinar cells and eventually induces pancreatitis in rats. DL-ethionine also inhibits incorporation of amino acids, like methionine and glycine, into rat protein in vivo and interferes with the use of adenosine triphosphate (ATP) in cells.

GoldBio’s DL39 (DE3) Chemically Competent E. coli cells are engineered with a T7 expression system to transform and express proteins in order to label residues such as phenylalanine or leucine. DL39 (DE3) Chemically Competent E. coli cells are deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases and are auxotrophic for aspartic acid, isoleucine, leucine, phenylananine, tyrosine, and valine residues.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: DL39
Species: E. coli
Transformation efficiency: ≥1 x 10⁷ cfu/µg pUC19 DNA
Blue/white screening: Yes

Yellow crystalline powder that is used for the colorimetric determination of thiol groups in biological samples. DTNB is fairly soluble in water.