Q_Products
TrueBlack™ Lipofuscin Autofluorescence Quencher
Catalog ID | Size | Pricing | |
---|---|---|---|
TB-250-1 | 1 mL | $ 169.00 |
Description
TrueBlack™ is a new alternative to Sudan Black B for the quenching of lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy and accumulate in a variety of cell and tissue types with age. It’s necessary to quench or mask lipofuscin fluorescence in certain adult human tissues or aged animal tissues in order to properly image specific immunofluorescence signals.
Sudan Black B has traditionally been used to quench lipofuscin autofluorescence by incubating tissue sections with the dye after immunofluorescence staining. However it also introduces uniform, non-specific background fluorescence in the red and far-red channels, limiting the use of fluorescent dyes in those wavelengths.
Product Specifications
Catalog ID | TB-250 |
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Storage/Handling | Store at room temperature. |
UN Number | 2265 |
Item Class | 3 |
Group Number | III |
Goof-Proof™ qPCR Master Mix
Catalog ID | Size | Pricing | |
---|---|---|---|
G-700-5 | 5 x 1 mL | $ 260.00 | |
G-700-1 | 1 mL | $ 260.00 |
Description
Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue (see Figure 2).
Goof-Proof™ allows you to see quickly and easily whether you forgot to add the master mix or the template to any of your reactions, preventing pipetting mistakes and wasted time, reagents and money. The 2X Goof-Proof™ Master Mix can also be used without the Template Buffer, if desired.
Product Specifications
Catalog ID | G-700 |
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Goof-Proof™ qPCR Master Mix with ROX
Catalog ID | Size | Pricing | |
---|---|---|---|
G-705-1 | 1 mL | $ 76.00 | |
G-705-5 | 5 x 1 mL | $ 260.00 |
Description
Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue.
Product Specifications
Catalog ID | G-705 |
---|---|
Storage/Handling | Store the kit at -20°C. |