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Competent Cells and Transformation
GoldBio offers an extensive collection of efficient chemically competent and electrocompetent cells with high transformation efficiencies to match your specific downstream applications: general cloning, protein expression, library construction, and many more.
- GoldBio's wide range of chemically competent and electrocompetent cells are optimized to provide lot-to-lot consistency and up to 10X higher transformation efficiency.
- Our enhanced recovery medium (SOC) formulation accompanies all of GoldBio's competent cells.
- We carry multiple Endonuclease I-deficient (endA1) competent bacterial cell strains to suit your cloning needs and ensure your transformations result in high quality and yield of plasmid DNA.
- Choose from E. coli strains with different selectable markers and screening options including the classic blue/white selection.
- GoldBio cells are ideal for distinct downstream applications from routine cloning/subcloning and protein expression to the more complex library construction, and cloning of difficult targets.
- Our electrocompetent Agrobacterium cells contain an efficient T-DNA binary system for genetic material transfer into the host plant cells.
- GoldBio's amber suppressor (SupE) and non-amber suppressor (MC1061F\’) strains are ideal for phage display library screening.
CHOOSE THE BEST COMPETENT CELLS FOR YOUR APPLICATION!
Our DH5-alpha, BL21, BL21(DE3), DH10B, DH10B-Pro, DL39(DE3), Agrobacterium, L. lactis, SS320 and TG1 Phage Display cells are available in multiple formats to provide you with flexibility when planning your experiments. GoldBio also provides easy-to-follow protocols, FAQs, protocol videos and selection guides to ensure success of your transformations!
Use GoldBio's Competent Cell Guide to Select the Right Competent Cells for your Assay
Includes transformation efficiencies, genotype, strain information, screening methods and applicatio...
Read more!
GoldBio Competent Cells Consistently Exhibit Higher Transformation Efficiencies
DH10B chemically competent E. coli cells and DH10B Electrocompetent E. coli cells exhibit higher transformation efficiency than competitors. Each transformation with DH10B chemically competent E. coli cells was performed using 1 ml of final volume including recovery media, plating 50 μl after a 10 fold-dilution (left). For electrocompetent cell comparison, DH10B Electrocompetent E. coli cells were electroporated using a 1 ml of final volume including recovery media, plating 50 μl after 100-fold dilution (right).
BROWSE OUR TRANSFORMATION AND COMPETENT CELL RESOURCES
Your time in the lab is valuable! In this section you will find GoldBio's transformation tools designed to help support and simplify your competent cell transformations. You can access specific chemical transformation or electroporation protocols for each of our competent cell lines, antibiotic information, colony screening protocols, instructional blog articles, detailed guides containing competent cells characteristics and downstream applications, FAQs, electroporation troubleshooting tips and video protocols.
Electroporation and Electrocompetent Cells FAQs:
What is electroporation?
Electroporation is a technique commonly used in transformation assays to introduce DNA into an electrocompetent cell. During electroporation a transient current or electrical pulse is applied to cells that have been suspended in a solution. The current momentarily increases the permeability of the cell's membrane, creating holes (a pathway) that allow DNA (plasmids) to pass through and enter the cell. Generally, electroporation can be more effective than chemical transformation.
When should I use electrocompetent cells?
Electrocompetent cells have a higher transformation efficiency than chemically competent cells. The use of electrocompetent cells facilitate the transformation of large plasmids, including BACs (Bacterial Artificial Chromosomes), that might otherwise be difficult to transform using chemical transformation.
How do I prevent arcing during electroporation?
Arcing during electroporation can be avoided by making sure there are no air bubbles in the cell/DNA solution prior to electroporation. Also, desalt your DNA since the presence of conductive ions in the solution will cause arcing and electroporation failure. In addition, ensure that your equipment, including the cuvette is properly cleaned and in good condition.
Showing 1-20 of 23 items.
AGL-1 Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-208-5x50 | 5 x 50 μL | $ 233.00 | |
| CC-208-10x50 | 10 x 50 μL | $ 430.00 | |
| CC-208-15x50 | 15 x 50 μL | $ 617.00 |
Description
GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells are optimized to produce the highest transformation efficiency, and are ideal for applications requiring high transformation efficiencies such as cDNA or gDNA library construction. The AGL-1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicillin resistance in its genome for selection. AGL-1 contains the Ti plasmid pTiBo542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
AGL1 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-106-5x50 | 5 x 50 μL | $ 227.00 | |
| CC-106-10x50 | 10 x 50 μL | $ 408.00 | |
| CC-106-15x50 | 15 x 50 μL | $ 599.00 |
Description
GoldBio’s AGL-1 Agrobacterium chemically competent cells are optimized to produce the highest transformation efficiency, and are ideal for applications requiring high transformation efficiencies such as cDNA or gDNA library construction. The AGL-1 strain has a C58 chromosomal background that carries an insertion mutation in its recA recombination gene which stabilizes recombinant plasmids. It also carries rifampicin and carbenicillin resistance in its genome for selection. AGL-1 contains the Ti plasmid pTiBo542 from which the T-DNA region sequences have been deleted. Transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of Arabidopsis thaliana as well as maize and other monocots.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: AGL-1
Format: Tubes
Transformation efficiency: ≥1 x 105 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
BL21 (DE3) Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-103-5x50 | 5 x 50 μL | $ 85.00 | |
| CC-103-10x50 | 10 x 50 μL | $ 129.00 | |
| CC-103-5x200 | 5 x 200 μL | $ 149.00 | |
| CC-103-15x50 | 15 x 50 μL | $ 167.00 | |
| CC-103-20x50 | 20 x 50 μL | $ 207.00 | |
| CC-103-10x200 | 10 x 200 μL | $ 253.00 | |
| CC-103-15x200 | 15 x 200 μL | $ 358.00 | |
| CC-103-20x200 | 20 x 200 μL | $ 462.00 |
Description
GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are suitable for transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.
Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes
BL21 (DE3) Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-204-5x50 | 5 x 50 μL | $ 129.00 | |
| CC-204-5x100 | 5 x 100 μL | $ 207.00 | |
| CC-204-10x50 | 10 x 50 μL | $ 223.00 | |
| CC-204-15x50 | 15 x 50 μL | $ 307.00 | |
| CC-204-10x100 | 10 x 100 μL | $ 355.00 | |
| CC-204-15x100 | 15 x 100 μL | $ 499.00 |
Description
GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 107 cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.
Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
BL21 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-102-5x50 | 5 x 50 μL | $ 71.00 | |
| CC-102-10x50 | 10 x 50 μL | $ 125.00 | |
| CC-102-15x50 | 15 x 50 μL | $ 156.00 |
Description
GoldBio’s BL21 Chemically Competent E. coli cells are suitable for transformation and routine protein expression from non-T7 vectors. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).
Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1.9 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
C58C1 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-109-5x50 | 5 x 50 μL | $ 227.00 | |
| CC-109-10x50 | 10 x 50 μL | $ 408.00 | |
| CC-109-15x50 | 15 x 50 μL | $ 599.00 |
Description
GoldBio’s C58C1 Chemically Competent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: C58C1
Format: Tubes
Transformation efficiency: ≥1 x 105 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
C58C1 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-240-5x50 | 5 x 50 μL | $ 233.00 | |
| CC-240-10x50 | 10 x 50 μL | $ 430.00 | |
| CC-240-15x50 | 15 x 50 μL | $ 617.00 |
Description
GoldBio’s C58C1 ElectroCompetent Agrobacterium cells are optimized for the highest transformation efficiency and are useful for various applications. The chromosomal background of C58C1 is C58. C58 is cured of the Ti plasmid pTiC58 resulting in C58C1. C58C1 Competent cells may be useful for transgenic operations that involve Arabidopsis and other plants. This Agrobacterium strain is streptomycin and rifampicin resistant.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: C58C1
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Competent Cell Recovery Medium
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-300-A | 6 x 12 ml | $ 80.00 |
Description
GoldBio’s Competent Cell Recovery Medium has a superior formulation recommended for all competent cell transformation. Our Recovery Medium is formulated to achieve the highest possible transformation efficiency from any competent cell.
- Achieve the highest possible transformation efficiency from any competent cell
- Enhanced SOC formulation provided with all of Intact Genomics’ competent cells
DH10B Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-100-5x50 | 5 x 50 μL | $ 84.00 | |
| CC-100-5x100 | 5 x 100 μL | $ 107.00 | |
| CC-100-10x50 | 10 x 50 μL | $ 129.00 | |
| CC-100-5x200 | 5 x 200 μL | $ 164.00 | |
| CC-100-15x50 | 15 x 50 μL | $ 169.00 | |
| CC-100-10x100 | 10 x 100 μL | $ 179.00 | |
| CC-100-20x50 | 20 x 50 μL | $ 215.00 | |
| CC-100-15x100 | 15 x 100 μL | $ 239.00 | |
| CC-100-10x200 | 10 x 200 μL | $ 284.00 | |
| CC-100-20x100 | 20 x 100 μL | $ 305.00 | |
| CC-100-15x200 | 15 x 200 μL | $ 406.00 |
Description
GoldBio’s DH10B Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of applications such as cloning and sub-cloning. DH10B Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.
Product Specifications
Competent cell type: Chemically competent
Derivative of: DH10B™
Species: E. coli
Format: Tubes or Plate (96 x 20 µl)
Transformation efficiency: ≥1.9 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
DH10B Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-200-5x50 | 5 x 50 μL | $ 139.00 | |
| CC-200-5x100 | 5 x 100 μL | $ 184.00 | |
| CC-200-10x50 | 10 x 50 μL | $ 233.00 | |
| CC-200-10x100 | 10 x 100 μL | $ 319.00 | |
| CC-200-15x50 | 15 x 50 μL | $ 326.00 | |
| CC-200-15x100 | 15 x 100 μL | $ 468.00 |
Description
GoldBio’s DH10B Electrocompetent E. coli cells offer the highest transformation efficiencies of ≥1 x 107 cfu/µg plasmid DNA, which are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. DH10B Electrocompetent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.
Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
| Catalog ID | CC-200 |
|---|---|
| Storage/Handling | Store Competent Cells at -80°C. |
DH10B-Pro™ Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-201-5x50 | 5 x 50 μL | $ 159.00 | |
| CC-201-5x100 | 5 x 100 μL | $ 315.00 | |
| CC-201-10x100 | 10 x 100 μL | $ 580.00 | |
| CC-201-15x100 | 15 x 100 μL | $ 842.00 |
Description
GoldBio’s DH10B-Pro™ Electrocompetent E. coli cells are suitable for demanding cloning applications requiring the greatest number of transformants, including synthetic bio-applications, BAC cloning, assembling large and multi-DNA fragments, and large construct transformation or cloning difficult targets. Utilizing proprietary manufacturing methods, these cells allow for effective transformation of all large DNA molecules (≥10 kb up to 350 kb!).
Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Format: Tubes
Transformation efficiency:≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
DH5-alpha Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-101-5x50 | 5 x 50 μL | $ 71.00 | |
| CC-101-5x100 | 5 x 100 μL | $ 111.00 | |
| CC-101-10x50 | 10 x 50 μL | $ 127.00 | |
| CC-101-15x50 | 15 x 50 μL | $ 172.00 | |
| CC-101-5x200 | 5 x 200 μL | $ 172.00 | |
| CC-101-10x100 | 10 x 100 μL | $ 180.00 | |
| CC-101-20x50 | 20 x 50 μL | $ 219.00 | |
| CC-101-15x100 | 15 x 100 μL | $ 242.00 | |
| CC-101-10x200 | 10 x 200 μL | $ 286.00 | |
| CC-101-15x200 | 15 x 200 μL | $ 405.00 |
Description
GoldBio’s DH5-alpha Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.
Product Specifications
Competent cell type: Chemically Competent
Derivative of: DH5-alpha
Species: E. coli
Format: Tubes
or Plates (96 x 20 µl)
Transformation efficiency: ≥1.9 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
DH5-alpha Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-203-5x50 | 5 x 50 μL | $ 141.00 | |
| CC-203-5x100 | 5 x 100 μL | $ 188.00 | |
| CC-203-10x50 | 10 x 50 μL | $ 246.00 | |
| CC-203-10x100 | 10 x 100 μL | $ 326.00 | |
| CC-203-15x50 | 15 x 50 μL | $ 341.00 | |
| CC-203-15x100 | 15 x 100 μL | $ 476.00 |
Description
GoldBio’s DH5-alpha Electrocompetent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning. Mutations in endA1 and recA1 ensure increased plasmid yield and improved plasmid quality.
Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH5-alpha
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
DL39 (DE3) Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-104-5x200 | 5 x 200 μL | $ 395.00 | |
| CC-104-10x200 | 10 x 200 μL | $ 775.00 |
Description
GoldBio’s DL39 (DE3) Chemically Competent E. coli cells are specific for transformation and protein expression in order to uniformly and specifically label residues (e.g. phenylalanine or leucine). DL39 (DE3) Chemically Competent E. coli cells can also be used to reduce NMR cross-labeling via transaminase activity for valine, leucine, isoleucine, aspartate, phenylananine, tyrosine and tryptophan residues.These cells are deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases. These cells also contain the T7 expression system.
Product Specifications
Competent cell type: Chemically Competent
Derivative of: DL39
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
EHA105 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-108-5x50 | 5 x 50 μL | $ 227.00 | |
| CC-108-10x50 | 10 x 50 μL | $ 408.00 | |
| CC-108-15x50 | 15 x 50 μL | $ 599.00 |
Description
GoldBio’s EHA105 Agrobacterium chemically competent cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). Our EHA105 strain is a non-virulent version of the super-virulent A. tumefaciens A281. The native T-DNA is deleted (pTiBo542dT-DNA) but the strain is still capable of moving an alternate T-DNA in trans to the plant host using the vir genes remaining on this helper plasmid.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105
Format: Tubes
Transformation efficiency: ≥1 x 105 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-225-5x50 | 5 x 50 μL | $ 233.00 | |
| CC-225-10x50 | 10 x 50 μL | $ 430.00 | |
| CC-225-15x50 | 15 x 50 μL | $ 617.00 |
Description
GoldBio’s EHA105 Agrobacterium ElectroCompetent cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The EHA105 strain is useful for transgenic operations of rice, tobacco and other plants. EHA105 contains a rifampicin resistance gene (rif). Our EHA105 strain is a non-virulent version of the super-virulent A. tumefaciens A281. The native T-DNA is deleted (pTiBo542dT-DNA) but the strain is still capable of moving an alternate T-DNA in trans to the plant host using the vir genes remaining on this helper plasmid.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-105-5x50 | 5 x 50 μL | $ 227.00 | |
| CC-105-10x50 | 10 x 50 μL | $ 408.00 | |
| CC-105-15x50 | 15 x 50 μL | $ 599.00 |
Description
GoldBio’s GV3101 Agrobacterium chemically competent cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as cDNA or gDNA library construction. The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pmp90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant S genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato and soybeans as well as monocots like corn.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101
Format: Tubes
Transformation efficiency: ≥1 x 105 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-207-5x50 | 5 x 50 μL | $ 233.00 | |
| CC-207-10x50 | 10 x 50 μL | $ 430.00 | |
| CC-207-15x50 | 15 x 50 μL | $ 617.00 |
Description
GoldBio’s GV3101 Agrobacterium Electrocompetent Cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as cDNA or gDNA library construction. The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pmp90 (pTiC58DT-DNA) with gentamicin resistance. GV3101 is also resistant to Chloramphenicol and this antibiotic cannot be used for selection. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant S genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato and soybeans as well as monocots like corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
i7® High-Fidelity DNA Polymerase 2X Master Mix
i7® High-Fidelity DNA Polymerase 2X Master Mix
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| D-922-100 | 500 Reactions | $ 175.00 | |
| D-922-500 | 500 Reactions | $ 599.00 |
Description
i7® High-Fidelity DNA Polymerase 2X Master Mix is ready to use premix which contains i7 high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. This 2X master mix product is supplied with 5X GC enhancer that enables efficient amplification of GC rich templates up to 84%.
Applications
- Long and difficult template DNA amplification
- Cloning
- High-fidelity PCR
- Efficient for amplifying high GC content template DNA with GC enhancer.
i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix
i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| D-923-100 | 500 Reactions | $ 195.00 | |
| D-923-500 | 500 Reactions | $ 609.00 |
Description
i7® Hot Start High-Fidelity DNA Polymerase 2X Master Mix is ready to use premix which contains hot start i7® high-fidelity DNA Polymerase, dNTPs, MgCl2, PCR enhancers and stabilizers with optimized proprietary reaction buffer. i7® Hot Start High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 102-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. This 2X master mix product is supplied with 5X GC enhancer that enables efficient amplification of GC rich templates up to 84%.
Applications
- Long and difficult template DNA amplification
- Cloning
- High-fidelity PCR
- Efficient for amplifying high GC content template DNA with GC enhancer.
