Competent Cells and Transformation

The 3xERRE-luciferase plasmid is designed to study the activity of the Estrogen-Related Receptor (ERR) signaling pathway, specifically its role in transcriptional regulation. This plasmid contains three copies of the Estrogen-Related Receptor Response Element (ERRE), which is a DNA sequence recognized by the estrogen-related receptor (ERR) family of transcription factors. ERK/MAPK regulates ERRgamma expression, transcriptional activity, and receptor-mediated Tamoxifen resistance in ER+ breast cancer.

The 3xERRE-luciferase plasmid is a synthetic plasmid obtained from a third party and its sequence has been confirmed at GoldBio

The 4xCSL-luciferase plasmid is designed to study the Notch signaling pathway by monitoring the transcriptional activity of CSL (CBF1/Suppressor of Hairless/LAG-1), a key transcription factor in the Notch pathway.

Murine notch homologs (N1-4) undergo presenilin-dependent proteolysis.

The 4xCSL-luciferase plasmid is a synthetic plasmid obtained from a third party and its sequence has been confirmed at Goldbio.

GoldBio’s AGL-1 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.

AGL-1 (pSoup) chemically competent cells are free of animal-derived products and grown with animal-free media.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the AGL-1 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: AGL-1 (pSoup)
Transformation efficiency: ≥3 x 10³ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

AGL-1 (pSoup) electrocompetent Agrobacterium cells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-type Agrobacterium strain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, helping to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1 (pSoup)
Transformation efficiency: ≥2 x 10⁸ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s AGL-1 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes and increasing the range of applications for the AGL-1 (pSoup-P19) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: AGL-1 (pSoup-P19)
Transformation efficiency: ≥2 x 10³ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

AGL-1 (pSoup-P19) electrocompetent Agrobacterium cells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-type Agrobacterium strain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1 (pSoup-P19)
Transformation efficiency: ≥1 x 10⁸ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥4 x 10⁴ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥5 x 10⁷ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s Agrobacterium Competent Cell Recovery Medium is an animal-free recovery medium specifically formulated for optimal competent cell transformations for Agrobacterium competent cells.

GoldBio’s new Auxo-Agro® competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.

Our EHA105 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.

GoldBio’s EHA105 Agrobacterium electrocompetent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain harbors a rifampicin resistance (rif) gene.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105∆Met
Format: Tubes
Transformation efficiency: ≥1.6 x 10⁸ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Transformation efficiency: ≥5 x 10⁵ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 10⁸ cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 10⁸ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s BL21 Chemically Competent E. coli cells are a good, universal strain of cell that is gives great transformation for protein expression and induction, and is especially useful for non-T7 vectors protein expression systems. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 10⁶ cfu/µg pUC19 DNA
Blue/white screening: Yes

GoldBio’s C58C1 Chemically Competent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s C58C1 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

pSuperAgro™ v4 was designed to include AcdS and GabT activity, driven by a single lac promotor, which both suppresses ethylene evolution and degrades gamma-aminobutyric acid (GABA) during co-cultivation. The combined suppression of ethylene and reduction of GABA significantly increases T-DNA transfer and increases transient and stable transformation frequencies in both tomato and grass plants.

These products are sold under license by GoldBio and require a signed agreement before fulfilment. The purchase of this product includes a 1-year subscription to use pSuperAgro™ in your research. See license details below in the technical documentation.

GoldBio’s C58C1 Chemically Competent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 10⁵ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s C58C1 Electrocompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

Product Specifications
Competent cell type: Electrocompetent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 10⁷ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio CJ236 chemically competent E. coli cells are ideally suited for use in the Kunkel method of mutagenesis. CJ236 cells have part of their Thymine replaced with deoxyuracil in the DNA due to dut- and ung- mutations which prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. CJ236 contains the F’ gene which confers Chloramphenicol resistance.

CJ236 chemically competent E. coli cells are free of animal-derived products and grown with animal-free media.

Kit Components

• Competent Cells
• 1 x 12 mL Recovery Media
• 1 x 10 µL Control Plasmid (pUC19 Control, 500 pg/µL)