Competent Cells and Transformation

GoldBio offers an extensive collection of efficient chemically competent and electrocompetent cells with high transformation efficiencies to match your specific downstream applications: general cloning, protein expression, library construction, and many more.

  • GoldBio's wide range of chemically competent and electrocompetent cells are optimized to provide lot-to-lot consistency and up to 10X higher transformation efficiency.
  • Our universal recovery medium (SOC) formulation accompanies ALL of GoldBio's competent cells.
  • We carry multiple Endonuclease I-deficient (endA1) competent bacterial cell strains to suit your cloning needs and ensure your transformations result in high quality and yield of plasmid DNA.
  • Choose from E. coli strains with different selectable markers and screening options including the classic blue/white selection.
  • GoldBio cells are ideal for distinct downstream applications from routine cloning/subcloning and protein expression to the more complex library construction, and cloning of difficult targets.
  • Our electrocompetent Agrobacterium cells contain an efficient T-DNA binary system for genetic material transfer into the host plant cells.

CHOOSE THE BEST COMPETENT CELLS FOR YOUR APPLICATION!

Our GB5-alpha™, BL21, BL21(DE3), GB10B™, GB10B-Pro™, DL39(DE3), and Agrobacterium cells are available in multiple formats to provide you with flexibility when planning your experiments. GoldBio also provides easy-to-follow protocols, FAQs, protocol videos and selection guides to ensure success of your transformations!

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BROWSE OUR TRANSFORMATION AND COMPETENT CELL RESOURCES

Your time in the lab is valuable! In this section you will find GoldBio's transformation tools designed to help support and simplify your competent cell transformations. You can access specific chemical transformation or electroporation protocols for each of our competent cell lines, antibiotic information, colony screening protocols, instructional blog articles, detailed guides containing competent cells characteristics and downstream applications, FAQs, electroporation troubleshooting tips and video protocols.

Electroporation and Electrocompetent Cells FAQs:

What is electroporation?

Electroporation is a technique commonly used in transformation assays to introduce DNA into an electrocompetent cell. During electroporation a transient current or electrical pulse is applied to cells that have been suspended in a solution. The current momentarily increases the permeability of the cell's membrane, creating holes (a pathway) that allow DNA (plasmids) to pass through and enter the cell. Generally, electroporation can be more effective than chemical transformation.

When should I use electrocompetent cells?

Electrocompetent cells have a higher transformation efficiency than chemically competent cells. The use of electrocompetent cells facilitate the transformation of large plasmids, including BACs (Bacterial Artificial Chromosomes), that might otherwise be difficult to transform using chemical transformation.

How do I prevent arcing during electroporation?

Arcing during electroporation can be avoided by making sure there are no air bubbles in the cell/DNA solution prior to electroporation. Also, desalt your DNA since the presence of conductive ions in the solution will cause arcing and electroporation failure. In addition, ensure that your equipment, including the cuvette is properly cleaned and in good condition.

Showing 1-20 of 37 items.

AGL-1 (pSoup) Agrobacterium Chemically Competent Cells

Catalog ID Size Pricing
CC-116-5x50 5 x 50 μL $ 189.00   $ 152.00
CC-116-10x50 10 x 50 μL $ 335.00   $ 268.00
CC-116-15x50 15 x 50 μL $ 449.00   $ 360.00

Description

GoldBio’s AGL-1 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the AGL-1 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: AGL-1 (pSoup)
Transformation efficiency: ≥3 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-116-5x50 In stock 5 x 50 μL $ 189.00
$ 152.00
CC-116-10x50 In stock 10 x 50 μL $ 335.00
$ 268.00
CC-116-15x50 In stock 15 x 50 μL $ 449.00
$ 360.00

AGL-1 (pSoup) Agrobacterium Electrocompetent Cells

Catalog ID Size Pricing
CC-218-5x50 5 x 50 μL $ 199.00   $ 160.00
CC-218-10x50 10 x 50 μL $ 359.00   $ 288.00
CC-218-15x50 15 x 50 μL $ 485.00   $ 388.00

Description

AGL-1 (pSoup) electrocompetent Agrobacterium cells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-type Agrobacterium strain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, helping to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1 (pSoup)
Transformation efficiency: ≥2 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-218-5x50 In stock 5 x 50 μL $ 199.00
$ 160.00
CC-218-10x50 In stock 10 x 50 μL $ 359.00
$ 288.00
CC-218-15x50 In stock 15 x 50 μL $ 485.00
$ 388.00

AGL-1 (pSoup-P19) Agrobacterium Chemically Competent Cells

Catalog ID Size Pricing
CC-126-5x50 5 x 50 μL $ 189.00   $ 152.00
CC-126-10x50 10 x 50 μL $ 335.00   $ 268.00
CC-126-15x50 15 x 50 μL $ 449.00   $ 360.00

Description

GoldBio’s AGL-1 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes and increasing the range of applications for the AGL-1 (pSoup-P19) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: AGL-1 (pSoup-P19)
Transformation efficiency: ≥2 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-126-5x50 In stock 5 x 50 μL $ 189.00
$ 152.00
CC-126-10x50 In stock 10 x 50 μL $ 335.00
$ 268.00
CC-126-15x50 In stock 15 x 50 μL $ 449.00
$ 360.00

AGL-1 (pSoup-P19) Agrobacterium Electrocompetent Cells

Catalog ID Size Pricing
CC-228-5x50 5 x 50 μL $ 199.00   $ 160.00
CC-228-10x50 10 x 50 μL $ 359.00   $ 288.00
CC-228-15x50 15 x 50 μL $ 485.00   $ 388.00

Description

AGL-1 (pSoup-P19) electrocompetent Agrobacterium cells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-type Agrobacterium strain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1 (pSoup-P19)
Transformation efficiency: ≥1 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-228-5x50 In stock 5 x 50 μL $ 199.00
$ 160.00
CC-228-10x50 In stock 10 x 50 μL $ 359.00
$ 288.00
CC-228-15x50 In stock 15 x 50 μL $ 485.00
$ 388.00

AGL-1 Agrobacterium Chemically Competent Cells

Catalog ID Size Pricing
CC-106-5x50 5 x 50 μL $ 189.00   $ 152.00
CC-106-10x50 10 x 50 μL $ 335.00   $ 268.00
CC-106-15x50 15 x 50 μL $ 449.00   $ 360.00

Description

GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥4 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-106-5x50 In stock 5 x 50 μL $ 189.00
$ 152.00
CC-106-10x50 In stock 10 x 50 μL $ 335.00
$ 268.00
CC-106-15x50 In stock 15 x 50 μL $ 449.00
$ 360.00

AGL-1 Agrobacterium Electrocompetent Cells

Catalog ID Size Pricing
CC-208-5x50 5 x 50 μL $ 199.00   $ 160.00
CC-208-10x50 10 x 50 μL $ 359.00   $ 288.00
CC-208-15x50 15 x 50 μL $ 485.00   $ 388.00

Description

GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥5 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-208-5x50 In stock 5 x 50 μL $ 199.00
$ 160.00
CC-208-10x50 In stock 10 x 50 μL $ 359.00
$ 288.00
CC-208-15x50 In stock 15 x 50 μL $ 485.00
$ 388.00

Auxo-Agro™ EHA105 Electrocompetent Cells

Catalog ID Size Pricing
CC-268-5x50 5 x 50 μL $ 199.00   $ 160.00
CC-268-10x50 10 x 50 μL $ 359.00   $ 288.00
CC-268-15x50 15 x 50 μL $ 485.00   $ 388.00

Description

GoldBio’s new Auxo-Agro™ competent cells are methionine auxotrophic strains of Agrobacterium which reduce overgrowth during the infection process while increasing plant transformation efficiency.

Our EHA105 strain of Agrobacterium tumefaciens can be used in genetic transformation of tomato, tobacco and other plants. After transformation, antibiotics are commonly used to remove Agrobacterium. However, even in the presence of antibiotics, there can be overgrowth of the Agrobacterium strain which can create a more difficult experimental protocol. Auxo-Agro™ cells help to solve this problem when selection is performed in both minimal media without Methionine in combination with selective antibiotics, such as Timentin, Cefotaxime or Meropenem.

GoldBio’s EHA105 Agrobacterium electrocompetent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain harbors a rifampicin resistance (rif) gene.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105∆Met
Format: Tubes
Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-268-5x50 In stock 5 x 50 μL $ 199.00
$ 160.00
CC-268-10x50 In stock 10 x 50 μL $ 359.00
$ 288.00
CC-268-15x50 In stock 15 x 50 μL $ 485.00
$ 388.00

BL21 (DE3) Chemically Competent E. coli Cells

Catalog ID Size Pricing
CC-103-5x50 5 x 50 μL $ 59.00   $ 48.00
CC-103-10x50 10 x 50 μL $ 105.00   $ 84.00
CC-103-15x50 15 x 50 μL $ 139.00   $ 112.00
CC-103-20x50 20 x 50 μL $ 165.00   $ 132.00

Description

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Packs & Pricing
,
CC-103-5x50 In stock 5 x 50 μL $ 59.00
$ 48.00
CC-103-10x50 In stock 10 x 50 μL $ 84.00
CC-103-15x50 In stock 15 x 50 μL $ 139.00
$ 112.00
CC-103-20x50 In stock 20 x 50 μL $ 165.00
$ 132.00

BL21 (DE3) Electrocompetent E. coli Cells

Catalog ID Size Pricing
CC-204-5x50 5 x 50 μL $ 119.00   $ 96.00
CC-204-10x50 10 x 50 μL $ 215.00   $ 172.00
CC-204-15x50 15 x 50 μL $ 295.00   $ 236.00

Description

GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 108 cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Packs & Pricing
,
CC-204-5x50 In stock 5 x 50 μL $ 96.00
CC-204-10x50 In stock 10 x 50 μL $ 215.00
$ 172.00
CC-204-15x50 In stock 15 x 50 μL $ 295.00
$ 236.00

BL21 Chemically Competent E. coli Cells

Catalog ID Size Pricing
CC-102-5x50 5 x 50 μL $ 59.00   $ 48.00
CC-102-10x50 10 x 50 μL $ 105.00   $ 84.00
CC-102-15x50 15 x 50 μL $ 139.00   $ 112.00

Description

GoldBio’s BL21 Chemically Competent E. coli cells are a good, universal strain of cell that is gives great transformation for protein expression and induction, and is especially useful for non-T7 vectors protein expression systems. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes

View
Packs & Pricing
,
CC-102-5x50 1 week 5 x 50 μL $ 59.00
$ 48.00
CC-102-10x50 In stock 10 x 50 μL $ 84.00
CC-102-15x50 In stock 15 x 50 μL $ 139.00
$ 112.00

C58C1 Agrobacterium Chemically Competent Cells

Catalog ID Size Pricing
CC-109-5x50 5 x 50 μL $ 189.00   $ 152.00
CC-109-10x50 10 x 50 μL $ 335.00   $ 268.00
CC-109-15x50 15 x 50 μL $ 449.00   $ 360.00

Description

GoldBio’s C58C1 Chemically Competent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 105 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-109-5x50 In stock 5 x 50 μL $ 189.00
$ 152.00
CC-109-10x50 In stock 10 x 50 μL $ 335.00
$ 268.00
CC-109-15x50 In stock 15 x 50 μL $ 449.00
$ 360.00

C58C1 Agrobacterium ElectroCompetent Cells

Catalog ID Size Pricing
CC-240-5x50 5 x 50 μL $ 199.00   $ 160.00
CC-240-10x50 10 x 50 μL $ 359.00   $ 288.00
CC-240-15x50 15 x 50 μL $ 485.00   $ 388.00

Description

GoldBio’s C58C1 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-240-5x50 In stock 5 x 50 μL $ 199.00
$ 160.00
CC-240-10x50 In stock 10 x 50 μL $ 359.00
$ 288.00
CC-240-15x50 In stock 15 x 50 μL $ 485.00
$ 388.00

Competent Cell Recovery Medium

Catalog ID Size Pricing
CC-300-A 6 x 12 ml $ 80.00

Description

GoldBio’s Universal Competent Cell Recovery Medium is specifically formulated for optimal competent cell transformations of every kind of cell, including E. coli and Agrobacterium.

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Packs & Pricing
,
CC-300-A In stock 6 x 12 ml $ 80.00

DL39 (DE3) Chemically Competent E. coli Cells

Catalog ID Size Pricing
CC-104-5x50 5 x 50 μL $ 59.00   $ 48.00
CC-104-10x50 10 x 50 μL $ 105.00   $ 84.00

Description

GoldBio’s DL39 (DE3) Chemically Competent E. coli cells are engineered with a T7 expression system to transform and express proteins in order to label residues such as phenylalanine or leucine. DL39 (DE3) Chemically Competent E. coli cells are deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases and are auxotrophic for aspartic acid, isoleucine, leucine, phenylananine, tyrosine, and valine residues.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: DL39
Species: E. coli
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes

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Packs & Pricing
,
CC-104-5x50 1 week 5 x 50 μL $ 59.00
$ 48.00
CC-104-10x50 In stock 10 x 50 μL $ 84.00

EHA105 (pSoup) Agrobacterium Chemically Competent Cells

Catalog ID Size Pricing
CC-118-5x50 5 x 50 μL $ 189.00   $ 152.00
CC-118-10x50 10 x 50 μL $ 335.00   $ 268.00
CC-118-15x50 15 x 50 μL $ 449.00   $ 360.00

Description

GoldBio’s EHA105 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥5 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-118-5x50 1 week 5 x 50 μL $ 189.00
$ 152.00
CC-118-10x50 In stock 10 x 50 μL $ 335.00
$ 268.00
CC-118-15x50 In stock 15 x 50 μL $ 449.00
$ 360.00

EHA105 (pSoup) Agrobacterium ElectroCompetent Cells

Catalog ID Size Pricing
CC-235-5x50 5 x 50 μL $ 199.00   $ 160.00
CC-235-10x50 10 x 50 μL $ 359.00   $ 288.00
CC-235-15x50 15 x 50 μL $ 485.00   $ 388.00

Description

EHA105 (pSoup) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥4 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-235-5x50 In stock 5 x 50 μL $ 199.00
$ 160.00
CC-235-10x50 In stock 10 x 50 μL $ 359.00
$ 288.00
CC-235-15x50 In stock 15 x 50 μL $ 485.00
$ 388.00

EHA105 (pSoup-P19) Agrobacterium Chemically Competent Cells

Catalog ID Size Pricing
CC-128-5x50 5 x 50 μL $ 189.00   $ 152.00
CC-128-10x50 10 x 50 μL $ 335.00   $ 268.00
CC-128-15x50 15 x 50 μL $ 449.00   $ 360.00

Description

GoldBio’s EHA105 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥2 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-128-5x50 In stock 5 x 50 μL $ 189.00
$ 152.00
CC-128-10x50 In stock 10 x 50 μL $ 335.00
$ 268.00
CC-128-15x50 In stock 15 x 50 μL $ 449.00
$ 360.00

EHA105 (pSoup-P19) Agrobacterium ElectroCompetent Cells

Catalog ID Size Pricing
CC-245-5x50 5 x 50 μL $ 199.00   $ 160.00
CC-245-10x50 10 x 50 μL $ 359.00   $ 288.00
CC-245-15x50 15 x 50 μL $ 485.00   $ 388.00

Description

EHA105 (pSoup-P19) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥3 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-245-5x50 In stock 5 x 50 μL $ 199.00
$ 160.00
CC-245-10x50 In stock 10 x 50 μL $ 359.00
$ 288.00
CC-245-15x50 In stock 15 x 50 μL $ 485.00
$ 388.00

EHA105 Agrobacterium Chemically Competent Cells

Catalog ID Size Pricing
CC-108-5x50 5 x 50 μL $ 189.00   $ 152.00
CC-108-10x50 10 x 50 μL $ 335.00   $ 268.00
CC-108-15x50 15 x 50 μL $ 449.00   $ 360.00

Description

GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥5 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-108-5x50 1 week 5 x 50 μL $ 189.00
$ 152.00
CC-108-10x50 In stock 10 x 50 μL $ 335.00
$ 268.00
CC-108-15x50 In stock 15 x 50 μL $ 449.00
$ 360.00

EHA105 Agrobacterium ElectroCompetent Cells

Catalog ID Size Pricing
CC-225-5x50 5 x 50 μL $ 199.00   $ 160.00
CC-225-10x50 10 x 50 μL $ 359.00   $ 288.00
CC-225-15x50 15 x 50 μL $ 485.00   $ 388.00

Description

GoldBio’s EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-225-5x50 In stock 5 x 50 μL $ 199.00
$ 160.00
CC-225-10x50 In stock 10 x 50 μL $ 359.00
$ 288.00
CC-225-15x50 In stock 15 x 50 μL $ 485.00
$ 388.00

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