Articles

Understanding Auxotrophic Mutants: Yeast and Bacterial Cloning Tools

Auxotrophic mutants are bacteria, yeast, protoplast or mammalian host cell strains that can’t produc...

E. coli Cloning Strains for Molecular Biology: What They Are and Why They Matter

Cloning strains are very specific bacterial strains used in molecular biology to clone recombinant D...

All About Differential Media in Gene Cloning - What it is and How it Works

Differential media is culture media containing compounds that enable visual distinction between the...

Selective Media Used in Gene Cloning

Selective media helps researchers identify their desired mutant host cell colonies during gene cloni...

Basic and Advanced Fluorescence Microscopy Techniques

Fluorescence microscopy spans from basic epifluorescence/widefield microscopy to advanced techniques...

Fluorescence microscopy: A Basic Introduction

Fluorescence microscopy is a fluorescence-based imaging technique. The basic principle involves stim...

The Basic Requirements for Gene Cloning

The requirements for successful gene cloning to consider are the general steps; the fundamental supp...

Troubleshooting SDS-PAGE Gel Running Issues

There are seven different indications of problems with SDS-PAGE gel running. Some of these include s...

Troubleshooting SDS-PAGE Sample Preparation Issues

There are five different signs that indicate that there are problems with SDS-PAGE sample preparatio...