Detergents & Membrane Agents
Surfactants play a vital role in protein biology by mediating the solubilization of membrane proteins for subsequent purification and downstream analysis. Picking the right detergent will result in succesful extraction of lipid bilayer-embedded proteins without affecting their function or structure. GoldBio has developed a large collection of ionic, non-ionic and zwitterionic detergents as well as bile acid salts to help you pick the right detergent for a succesful extraction of your lipid bylayer-embedded protein without affecting its function or structure. In addition, we provide various protein solubilization resources including protocols, troubleshooting tips and guides, to streamline your membrane solubilization process.
Our large variety of tested and purified detergents will yield reliable results when used in applications such as cell lysis, protein solubilization and crystallization, DNA extraction, preparation of liposomes, immunoassays and 2-D gel electrophoresis. Our mild nonionic and zwitterionic detergents like CHAPs, Octylglucloside and digitonin among others, are ideal for isolation of water-soluble proteins without destroying their structure or affecting their function. If your experiment requires complete denaturation of your target protein then you can browse our ionic detergents-cetylpyridinium chloride, chlorhexidine, hexadecylpyridinium bromide--which will effectively solubilize your membrane protein for downstream applications.
Find the information and resources to help pick and use the right detergent for your membrane protein characterization in our easy-to-follow protocols, stock solution recipes, troubleshooting tips and protease inhibitor guides. You can also discover detailed information on critical micelle concentration, micelle size and aggregation number as well as function and general properties in our Detergent Selection Guide. In addition, our in depth blog articles provide technical advice and more for optimization of your experimental protein biology procedures.