T_Products

TrueBlack™ is a new alternative to Sudan Black B for the quenching of lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy and accumulate in a variety of cell and tissue types with age. It’s necessary to quench or mask lipofuscin fluorescence in certain adult human tissues or aged animal tissues in order to properly image specific immunofluorescence signals.

Sudan Black B has traditionally been used to quench lipofuscin autofluorescence by incubating tissue sections with the dye after immunofluorescence staining. However it also introduces uniform, non-specific background fluorescence in the red and far-red channels, limiting the use of fluorescent dyes in those wavelengths.

1,2,3,4-Tetra-O-acetyl-β-D-glucuronic acid methyl ester has been used in various biochemical studies. It has been used to study the potential of water-soluble camptothecin glucuronide derivatives as a cancer prodrug monotherapy and antibody-directed enzyme prodrug therapy. Additionally, it has been used to assess the role of glycosylthio heterocycles in carbohydrates.

1,2,4-Triazole is an aromatic molecule. It is a reagent for the synthesis of many different molecules, including conazole based antifungals, ribofuranosyl based antivirals and antitumor agents.

1-O-Tetradecylglycerol is an emulsifying agent and chemical reagent.

2,3,4,6-Tetra-O-benzyl-D-glucopyranose is used for glucosylation reactions and as a reagent for synthesis of 1-C-alpha-D-glucopyranose derivatives.

2,3,4,6-Tetra-O-benzyl-D-mannopyranose is used for glucosylation reactions and as a reagent for synthesis of 1-C-alpha-D-glucopyranose derivatives.

Tetrazolium red is used to visualize dehydrogenase enzyme activity. Initially, the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions, which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.

2,4,6-Tris(2-pyridyl)-1,3,5-triazine or TPTZ is a triazine compound containing 3 pyridyl groups. It is commonly used as a reagent for the spectrophotometric determination of Fe(II) and total Fe. It can also be used to determine the amount of acetaminophen in samples through spectrophotometry.

2-thiouracil is a sulfated uracil typically used as an anti-thyroid agent for Graves’ disease by inhibiting the thyroid enzyme thyroid peroxidase. When given to lab animals it can induce arteriosclerosis.

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Blue Tetrazolium chloride is a chemical compound used for sensitive detection of alkaline phosphatase. With immunohistochemistry blue tetrazolium is used as a diagnostic for diseases of the phagocyte function by gauging the resulting blue color in the presence of oxygen formed by the phagocyte while killing bacteria.

D-Tryptophan is used in the liver and kidneys to produce d-kynurenine. It can be used as a reagent for chemical synthesis.

DL-3-Tyrosine has a hypotensive effect on blood pressure and is converted to dopamine in rats. It can also be used as a reagent for chemical synthesis.

Trypsin is a serine protease that hydrolyses proteins which can be found in the digestive system. Trypsin cleaves peptide bonds on the carboxy side of s-aminoethyl cysteine, arginine and lysine residues with very little or no cleavage at lysyl-proline and arginyl-proline bonds. This special cleaving activity yields peptides that are easily identified by mass spectrometry. Additionally, our Immobilized Trypsin is TPCK treated to deactivate interfering chymotrypsin, which can interfere with sequence analysis.

Irgasan (triclosan) is a broad-spectrum antifungal and antibiotic. It is often used as a supplement to cell culture media in Pseudomonas and Yersinia isolation agars to prevent bacterial contamination. It is slightly soluble in water and freely soluble in ethanol and methanol.

Irgasan binds the enoyl-acyl carrier protein reductase and forms a complex. Consequently, NAD (nicotinamide adenine dinucleotide) binds and prevents the enoyl-ACP complex from participating in the fatty acid synthesis cycle, suppressing bacterial cell growth.

L-Threonine 7-amido-4-methylcoumarin is an AMC fluorgenic substrate.

Tryptophan is an essential amino acid in the human diet. Its functions as a serotonin precursor have increased interest in its ability to treat depression and epilepsy; however, there has been inconclusive evidence in clinical trials regarding its efficacy in humans. Safety has also been a concern.

It is widely used in molecular genetics as an auxotrophic genetic marker in yeast gene disruption experiments.

L-Tyrosine 7-amido-4-methylcoumarin is used as an AMC fluorogenic substrate.