AGL-1 (pSoup) Agrobacterium Chemically Competent Cells

1
Catalog ID Size Pricing
CC-116-5x50 5 x 50 μL $ 189.00   $ 161.00
CC-116-10x50 10 x 50 μL $ 335.00   $ 285.00
CC-116-15x50 15 x 50 μL $ 449.00   $ 382.00

Description

GoldBio’s AGL-1 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the AGL-1 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: AGL-1 (pSoup)
Transformation efficiency: ≥3 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-116-5x50 In stock 5 x 50 μL $ 189.00
$ 161.00
CC-116-10x50 In stock 10 x 50 μL $ 335.00
$ 285.00
CC-116-15x50 In stock 15 x 50 μL $ 449.00
$ 382.00

AGL-1 (pSoup) Agrobacterium Electrocompetent Cells

1
Catalog ID Size Pricing
CC-218-5x50 5 x 50 μL $ 199.00   $ 170.00
CC-218-10x50 10 x 50 μL $ 359.00   $ 306.00
CC-218-15x50 15 x 50 μL $ 485.00   $ 413.00

Description

AGL-1 (pSoup) electrocompetent Agrobacterium cells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-type Agrobacterium strain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, helping to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1 (pSoup)
Transformation efficiency: ≥2 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-218-5x50 In stock 5 x 50 μL $ 199.00
$ 170.00
CC-218-10x50 In stock 10 x 50 μL $ 359.00
$ 306.00
CC-218-15x50 In stock 15 x 50 μL $ 485.00
$ 413.00

AGL-1 (pSoup-P19) Agrobacterium Chemically Competent Cells

1
Catalog ID Size Pricing
CC-126-5x50 5 x 50 μL $ 189.00   $ 161.00
CC-126-10x50 10 x 50 μL $ 335.00   $ 285.00
CC-126-15x50 15 x 50 μL $ 449.00   $ 382.00

Description

GoldBio’s AGL-1 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes and increasing the range of applications for the AGL-1 (pSoup-P19) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: AGL-1 (pSoup-P19)
Transformation efficiency: ≥2 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-126-5x50 In stock 5 x 50 μL $ 189.00
$ 161.00
CC-126-10x50 In stock 10 x 50 μL $ 335.00
$ 285.00
CC-126-15x50 In stock 15 x 50 μL $ 449.00
$ 382.00

AGL-1 (pSoup-P19) Agrobacterium Electrocompetent Cells

1
Catalog ID Size Pricing
CC-228-5x50 5 x 50 μL $ 199.00   $ 170.00
CC-228-10x50 10 x 50 μL $ 359.00   $ 306.00
CC-228-15x50 15 x 50 μL $ 485.00   $ 413.00

Description

AGL-1 (pSoup-P19) electrocompetent Agrobacterium cells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-type Agrobacterium strain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1 (pSoup-P19)
Transformation efficiency: ≥1 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-228-5x50 In stock 5 x 50 μL $ 199.00
$ 170.00
CC-228-10x50 In stock 10 x 50 μL $ 359.00
$ 306.00
CC-228-15x50 In stock 15 x 50 μL $ 485.00
$ 413.00

AGL-1 Agrobacterium Chemically Competent Cells

CC-106 image AGL-1 Agrobacterium Chemically Competent Cells 1
Catalog ID Size Pricing
CC-106-5x50 5 x 50 μL $ 189.00   $ 161.00
CC-106-10x50 10 x 50 μL $ 335.00   $ 285.00
CC-106-15x50 15 x 50 μL $ 449.00   $ 382.00

Description

GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥4 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-106-5x50 In stock 5 x 50 μL $ 189.00
$ 161.00
CC-106-10x50 In stock 10 x 50 μL $ 335.00
$ 285.00
CC-106-15x50 In stock 15 x 50 μL $ 449.00
$ 382.00

AGL-1 Agrobacterium Electrocompetent Cells

CC-208 Image AGL-1 Agrobacterium Electrocompetent Cells 1
Catalog ID Size Pricing
CC-208-5x50 5 x 50 μL $ 199.00   $ 170.00
CC-208-10x50 10 x 50 μL $ 359.00   $ 306.00
CC-208-15x50 15 x 50 μL $ 485.00   $ 413.00

Description

GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥5 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-208-5x50 In stock 5 x 50 μL $ 199.00
$ 170.00
CC-208-10x50 In stock 10 x 50 μL $ 359.00
$ 306.00
CC-208-15x50 In stock 15 x 50 μL $ 485.00
$ 413.00

BL21 (DE3) Chemically Competent E. coli Cells

CC-103 image BL21 (DE3) Chemically Competent E. coli Cells 1
Catalog ID Size Pricing
CC-103-5x50 5 x 50 μL $ 59.00   $ 51.00
CC-103-10x50 10 x 50 μL $ 105.00   $ 90.00
CC-103-15x50 15 x 50 μL $ 139.00   $ 119.00
CC-103-20x50 20 x 50 μL $ 165.00   $ 141.00

Description

GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes

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Packs & Pricing
,
CC-103-5x50 In stock 5 x 50 μL $ 59.00
$ 51.00
CC-103-10x50 In stock 10 x 50 μL $ 90.00
CC-103-15x50 In stock 15 x 50 μL $ 139.00
$ 119.00
CC-103-20x50 In stock 20 x 50 μL $ 165.00
$ 141.00

BL21 (DE3) Electrocompetent E. coli Cells

CC-204 Image BL21 (DE3) Electrocompetent E. coli Cells 1
Catalog ID Size Pricing
CC-204-5x50 5 x 50 μL $ 119.00   $ 102.00
CC-204-10x50 10 x 50 μL $ 215.00   $ 183.00
CC-204-15x50 15 x 50 μL $ 295.00   $ 251.00

Description

GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 108 cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes

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Packs & Pricing
,
CC-204-5x50 In stock 5 x 50 μL $ 119.00
$ 102.00
CC-204-10x50 In stock 10 x 50 μL $ 215.00
$ 183.00
CC-204-15x50 In stock 15 x 50 μL $ 295.00
$ 251.00

BL21 Chemically Competent E. coli Cells

CC-102 image 1
Catalog ID Size Pricing
CC-102-5x50 5 x 50 μL $ 59.00   $ 51.00
CC-102-10x50 10 x 50 μL $ 105.00   $ 90.00
CC-102-15x50 15 x 50 μL $ 139.00   $ 119.00

Description

GoldBio’s BL21 Chemically Competent E. coli cells are a good, universal strain of cell that is gives great transformation for protein expression and induction, and is especially useful for non-T7 vectors protein expression systems. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).

Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes

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Packs & Pricing
,
CC-102-5x50 1 week 5 x 50 μL $ 59.00
$ 51.00
CC-102-10x50 In stock 10 x 50 μL $ 90.00
CC-102-15x50 In stock 15 x 50 μL $ 139.00
$ 119.00

C58C1 Agrobacterium Chemically Competent Cells

CC-109 image 1
Catalog ID Size Pricing
CC-109-5x50 5 x 50 μL $ 189.00   $ 161.00
CC-109-10x50 10 x 50 μL $ 335.00   $ 285.00
CC-109-15x50 15 x 50 μL $ 449.00   $ 382.00

Description

GoldBio’s C58C1 Chemically Competent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 105 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-109-5x50 In stock 5 x 50 μL $ 189.00
$ 161.00
CC-109-10x50 In stock 10 x 50 μL $ 335.00
$ 285.00
CC-109-15x50 In stock 15 x 50 μL $ 449.00
$ 382.00

C58C1 Agrobacterium ElectroCompetent Cells

CC-240 Image C58C1 Agrobacterium ElectroCompetent Cells 1
Catalog ID Size Pricing
CC-240-5x50 5 x 50 μL $ 199.00   $ 170.00
CC-240-10x50 10 x 50 μL $ 359.00   $ 306.00
CC-240-15x50 15 x 50 μL $ 485.00   $ 413.00

Description

GoldBio’s C58C1 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-240-5x50 In stock 5 x 50 μL $ 199.00
$ 170.00
CC-240-10x50 In stock 10 x 50 μL $ 359.00
$ 306.00
CC-240-15x50 In stock 15 x 50 μL $ 485.00
$ 413.00

Competent Cell Recovery Medium

CC-300 image Competent Cell Recovery Medium 1
Catalog ID Size Pricing
CC-300-A 6 x 12 ml $ 80.00   $ 68.00

Description

GoldBio’s Universal Competent Cell Recovery Medium is specifically formulated for optimal competent cell transformations of every kind of cell, including E. coli and Agrobacterium.

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Packs & Pricing
,
CC-300-A In stock 6 x 12 ml $ 80.00
$ 68.00

DH10B Chemically Competent E. coli Cells

CC-100 image DH10B Chemically Competent E. coli Cells 1
Catalog ID Size Pricing
CC-100-5x50 5 x 50 μL $ 59.00   $ 51.00
CC-100-5x100 5 x 100 μL $ 105.00   $ 90.00
CC-100-10x50 10 x 50 μL $ 105.00   $ 90.00
CC-100-15x50 15 x 50 μL $ 139.00   $ 119.00
CC-100-20x50 20 x 50 μL $ 165.00   $ 141.00
CC-100-10x100 10 x 100 μL $ 179.00   $ 153.00
CC-100-15x100 15 x 100 μL $ 239.00   $ 204.00
CC-100-20x100 20 x 100 μL $ 305.00   $ 260.00

Description

GoldBio’s DH10B Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. DH10B Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.

Product Specifications
Competent cell type: Chemically competent
Derivative of: DH10B™
Species: E. coli
Transformation efficiency: ≥8.2 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes

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Packs & Pricing
,
CC-100-5x50 In stock 5 x 50 μL $ 59.00
$ 51.00
CC-100-5x100 1 week 5 x 100 μL $ 90.00
CC-100-10x50 In stock 10 x 50 μL $ 90.00
CC-100-15x50 In stock 15 x 50 μL $ 139.00
$ 119.00
CC-100-20x50 In stock 20 x 50 μL $ 165.00
$ 141.00
CC-100-10x100 In stock 10 x 100 μL $ 179.00
$ 153.00
CC-100-15x100 In stock 15 x 100 μL $ 239.00
$ 204.00
CC-100-20x100 In stock 20 x 100 μL $ 305.00
$ 260.00

DH10B Electrocompetent E. coli Cells

CC-200 image DH10B Electrocompetent E. coli Cells 1
Catalog ID Size Pricing
CC-200-5x50 5 x 50 μL $ 119.00   $ 102.00
CC-200-5x100 5 x 100 μL $ 185.00   $ 158.00
CC-200-10x50 10 x 50 μL $ 215.00   $ 183.00
CC-200-15x50 15 x 50 μL $ 295.00   $ 251.00
CC-200-10x100 10 x 100 μL $ 319.00   $ 272.00
CC-200-15x100 15 x 100 μL $ 468.00   $ 398.00

Description

GoldBio’s DH10B Electrocompetent E. coli cells are high efficiency cells, ≥2 x 108 cfu/µg plasmid DNA, suitable for a wide variety of applications requiring high transformation efficiencies, such as cloning and subcloning, as well as working with either cDNA or gDNA library construction. DH10B Electrocompetent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine. Here, we present a detailed protocol for electroporation using DH10B Electrocompetent E. coli cells.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Transformation efficiency: ≥2 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes

Product Specifications

Catalog ID CC-200
Storage/Handling Store Competent Cells at -80°C.
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Packs & Pricing
,
CC-200-5x50 In stock 5 x 50 μL $ 119.00
$ 102.00
CC-200-5x100 In stock 5 x 100 μL $ 185.00
$ 158.00
CC-200-10x50 In stock 10 x 50 μL $ 215.00
$ 183.00
CC-200-15x50 In stock 15 x 50 μL $ 295.00
$ 251.00
CC-200-10x100 In stock 10 x 100 μL $ 319.00
$ 272.00
CC-200-15x100 In stock 15 x 100 μL $ 468.00
$ 398.00

DH10B-Pro™ Electrocompetent E. coli Cells

CC-201 Image DH10B-Pro™ Electrocompetent E. coli Cells 1
Catalog ID Size Pricing
CC-201-5x50 5 x 50 μL $ 119.00   $ 102.00
CC-201-10x50 10 x 50 μL $ 215.00   $ 183.00
CC-201-5x100 5 x 100 μL $ 215.00   $ 183.00
CC-201-15x50 15 x 50 μL $ 295.00   $ 251.00
CC-201-10x100 10 x 100 μL $ 580.00   $ 493.00
CC-201-15x100 15 x 100 μL $ 842.00   $ 716.00

Description

GoldBio’s DH10B-Pro™ Electrocompetent E. coli cells are especially designed for the most demanding cloning applications. DH10-Pro™ cells will provide the greatest number of transformants for when your research requires it, including assembling large and multi-DNA fragments, cloning large (≥10 kb up to 350 kb) or difficult construct transformations, working with synthetic bio-applications, and even BAC cloning.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH10B™
Species: E. coli
Transformation efficiency:≥1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes

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Packs & Pricing
,
CC-201-5x50 In stock 5 x 50 μL $ 119.00
$ 102.00
CC-201-10x50 In stock 10 x 50 μL $ 215.00
$ 183.00
CC-201-5x100 In stock 5 x 100 μL $ 215.00
$ 183.00
CC-201-15x50 In stock 15 x 50 μL $ 295.00
$ 251.00
CC-201-10x100 In stock 10 x 100 μL $ 580.00
$ 493.00
CC-201-15x100 In stock 15 x 100 μL $ 842.00
$ 716.00

DH5-alpha Chemically Competent E. coli Cells

CC-101 image DH5-alpha Chemically Competent E. coli Cells 1
Catalog ID Size Pricing
CC-101-5x50 5 x 50 μL $ 59.00   $ 51.00
CC-101-5x100 5 x 100 μL $ 105.00   $ 90.00
CC-101-10x50 10 x 50 μL $ 105.00   $ 90.00
CC-101-15x50 15 x 50 μL $ 139.00   $ 119.00
CC-101-20x50 20 x 50 μL $ 165.00   $ 141.00
CC-101-10x100 10 x 100 μL $ 180.00   $ 153.00
CC-101-15x100 15 x 100 μL $ 242.00   $ 206.00

Description

GoldBio’s DH5-alpha Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes

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Packs & Pricing
,
CC-101-5x50 In stock 5 x 50 μL $ 59.00
$ 51.00
CC-101-5x100 1 week 5 x 100 μL $ 90.00
CC-101-10x50 In stock 10 x 50 μL $ 90.00
CC-101-15x50 In stock 15 x 50 μL $ 139.00
$ 119.00
CC-101-20x50 In stock 20 x 50 μL $ 165.00
$ 141.00
CC-101-10x100 In stock 10 x 100 μL $ 180.00
$ 153.00
CC-101-15x100 In stock 15 x 100 μL $ 242.00
$ 206.00

DH5-alpha Electrocompetent E. coli Cells

CC-203 Image DH5-alpha Electrocompetent E. coli Cells 1
Catalog ID Size Pricing
CC-203-5x50 5 x 50 μL $ 119.00   $ 102.00
CC-203-5x100 5 x 100 μL $ 185.00   $ 158.00
CC-203-10x50 10 x 50 μL $ 215.00   $ 183.00
CC-203-15x50 15 x 50 μL $ 295.00   $ 251.00
CC-203-10x100 10 x 100 μL $ 315.00   $ 268.00
CC-203-15x100 15 x 100 μL $ 399.00   $ 340.00

Description

GoldBio’s DH5-alpha Electrocompetent E. coli are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. Plasmid quality and yields are both enhanced in our DH5-alpha cells due to mutations in both endA1 and recA1.

Product Specifications
Competent cell type: ElectroCompetent
Derivative of: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes

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Packs & Pricing
,
CC-203-5x50 In stock 5 x 50 μL $ 119.00
$ 102.00
CC-203-5x100 In stock 5 x 100 μL $ 185.00
$ 158.00
CC-203-10x50 In stock 10 x 50 μL $ 215.00
$ 183.00
CC-203-15x50 In stock 15 x 50 μL $ 295.00
$ 251.00
CC-203-10x100 In stock 10 x 100 μL $ 315.00
$ 268.00
CC-203-15x100 In stock 15 x 100 μL $ 399.00
$ 340.00

DL39 (DE3) Chemically Competent E. coli Cells

CC-104 image DL39 (DE3) Chemically Competent E. coli Cells 1
Catalog ID Size Pricing
CC-104-5x50 5 x 50 μL $ 59.00   $ 51.00
CC-104-10x50 10 x 50 μL $ 105.00   $ 90.00

Description

GoldBio’s DL39 (DE3) Chemically Competent E. coli cells are engineered with a T7 expression system to transform and express proteins in order to label residues such as phenylalanine or leucine. DL39 (DE3) Chemically Competent E. coli cells are deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases and are auxotrophic for aspartic acid, isoleucine, leucine, phenylananine, tyrosine, and valine residues.

Product Specifications
Competent cell type: Chemically Competent
Derivative of: DL39
Species: E. coli
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes

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Packs & Pricing
,
CC-104-5x50 In stock 5 x 50 μL $ 59.00
$ 51.00
CC-104-10x50 In stock 10 x 50 μL $ 90.00

EHA105 (pSoup) Agrobacterium Chemically Competent Cells

1
Catalog ID Size Pricing
CC-118-5x50 5 x 50 μL $ 189.00   $ 161.00
CC-118-10x50 10 x 50 μL $ 335.00   $ 285.00
CC-118-15x50 15 x 50 μL $ 449.00   $ 382.00

Description

GoldBio’s EHA105 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥5 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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Packs & Pricing
,
CC-118-5x50 1 week 5 x 50 μL $ 189.00
$ 161.00
CC-118-10x50 In stock 10 x 50 μL $ 335.00
$ 285.00
CC-118-15x50 In stock 15 x 50 μL $ 449.00
$ 382.00

EHA105 (pSoup) Agrobacterium ElectroCompetent Cells

1
Catalog ID Size Pricing
CC-235-5x50 5 x 50 μL $ 199.00   $ 170.00
CC-235-10x50 10 x 50 μL $ 359.00   $ 306.00
CC-235-15x50 15 x 50 μL $ 485.00   $ 413.00

Description

EHA105 (pSoup) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥4 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

View
Packs & Pricing
,
CC-235-5x50 In stock 5 x 50 μL $ 199.00
$ 170.00
CC-235-10x50 In stock 10 x 50 μL $ 359.00
$ 306.00
CC-235-15x50 In stock 15 x 50 μL $ 485.00
$ 413.00

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