Competent Cells
GoldBio offers high quality chemically competent and electrocompetent cells with the reliability and confidence that you have come to know from the GoldBio brand.
GoldBio competent cells are made and tested in our lab and are ideal for a variety of research use; including general cloning, protein expression and library construction.
GoldBio also provides an expansive array of supportive resources, articles, calculators and reagents to make your experiments more efficient and reliable.
GoldBio's electrocompetent cells are highly efficient, with transformation efficiencies that have been proven and verified in our labs to have a real efficiency up to ≥1 x 108 cfu/μg DNA. We personally test and validate every batch of our competent cells so that researchers have the highest degree of confidence in our cells and success in their experiments, saving valuable time and resources.
GB10B™ Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-200-5x50 | 5 x 50 μL | $ 119.00 | |
| CC-200-5x100 | 5 x 100 μL | $ 185.00 | |
| CC-200-10x50 | 10 x 50 μL | $ 215.00 | |
| CC-200-15x50 | 15 x 50 μL | $ 295.00 | |
| CC-200-10x100 | 10 x 100 μL | $ 319.00 | |
| CC-200-15x100 | 15 x 100 μL | $ 468.00 |
Description
GoldBio’s GB10B™ Electrocompetent E. coli cells are high efficiency cells, ≥2 x 108 cfu/µg plasmid DNA, suitable for a wide variety of applications requiring high transformation efficiencies, such as cloning and subcloning, as well as working with either cDNA or gDNA library construction. GB10B™ Electrocompetent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine. Here, we present a detailed protocol for electroporation using GB10B™ Electrocompetent E. coli cells.
Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH10B
Species: E. coli
Transformation efficiency: ≥2 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
| Catalog ID | CC-200 |
|---|---|
| Storage/Handling | Store Competent Cells at -80°C. |
GB10B-Pro™ Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-201-5x50 | 5 x 50 μL | $ 119.00 | |
| CC-201-10x50 | 10 x 50 μL | $ 215.00 | |
| CC-201-5x100 | 5 x 100 μL | $ 215.00 | |
| CC-201-15x50 | 15 x 50 μL | $ 295.00 | |
| CC-201-10x100 | 10 x 100 μL | $ 580.00 | |
| CC-201-15x100 | 15 x 100 μL | $ 842.00 |
Description
GoldBio’s GB10B-Pro™ Electrocompetent E. coli cells are especially designed for the most demanding cloning applications. GB10B-Pro™ cells will provide the greatest number of transformants for when your research requires it, including assembling large and multi-DNA fragments, cloning large (≥10 kb up to 350 kb) or difficult construct transformations, working with synthetic bio-applications, and even BAC cloning.
Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH10B
Species: E. coli
Transformation efficiency:≥1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes
GB5-alpha™ Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-203-5x50 | 5 x 50 μL | $ 119.00 | |
| CC-203-5x100 | 5 x 100 μL | $ 185.00 | |
| CC-203-10x50 | 10 x 50 μL | $ 215.00 | |
| CC-203-15x50 | 15 x 50 μL | $ 295.00 | |
| CC-203-10x100 | 10 x 100 μL | $ 315.00 | |
| CC-203-15x100 | 15 x 100 μL | $ 399.00 |
Description
GoldBio’s GB5-alpha™ Electrocompetent E. coli are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. Plasmid quality and yields are both enhanced in our GB5-alpha™ cells due to mutations in both endA1 and recA1.
Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes
BL21 (DE3) Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-204-5x50 | 5 x 50 μL | $ 119.00 | |
| CC-204-10x50 | 10 x 50 μL | $ 215.00 | |
| CC-204-15x50 | 15 x 50 μL | $ 295.00 |
Description
GoldBio’s BL21 (DE3) Electrocompetent E. coli cells are suitable for high efficiency transformation and routine protein expression. Increased cloning efficiencies versus typical BL21 cells makes the BL21 (DE3) Electrocompetent E. coli cells ideal for construction of complex expression libraries and feature ≥1 x 108 cfu/µg efficiency with electroporation. BL21 (DE3) Electrocompetent E. coli cells have a widely used background, feature a T7 expression strain, are deficient in both lon (1) and ompT proteases. These cells are resistant to phage T1(fhuA2) and are B strain.
Product Specifications
Competent cell type: ElectroCompetent
Derivative of: BL21 (DE3)
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes
GoldBio's competent cells are designed to work with a variety of cloning vectors, making them versatile and adaptable for a wide range of applications. GoldBio's chemically competent cells are proven and verified in our labs to have a real efficiency up to ≥1 x 107 cfu/μg DNA and come in convenient packaging sizes, from small-scale experiments to large-scale production runs, ensuring that researchers can find the right size for their needs.
GB10B™ Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-100-5x50 | 5 x 50 μL | $ 59.00 | |
| CC-100-5x100 | 5 x 100 μL | $ 105.00 | |
| CC-100-10x50 | 10 x 50 μL | $ 105.00 | |
| CC-100-15x50 | 15 x 50 μL | $ 139.00 | |
| CC-100-20x50 | 20 x 50 μL | $ 165.00 | |
| CC-100-10x100 | 10 x 100 μL | $ 179.00 | |
| CC-100-15x100 | 15 x 100 μL | $ 239.00 | |
| CC-100-20x100 | 20 x 100 μL | $ 305.00 |
Description
GoldBio’s GB10B™ Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. GB10B™ Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.
Product Specifications
Competent cell type: Chemically competent
Equivalent to: DH10B
Species: E. coli
Transformation efficiency: ≥8.2 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes
GB5-alpha™ Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-101-5x50 | 5 x 50 μL | $ 59.00 | |
| CC-101-5x100 | 5 x 100 μL | $ 105.00 | |
| CC-101-10x50 | 10 x 50 μL | $ 105.00 | |
| CC-101-15x50 | 15 x 50 μL | $ 139.00 | |
| CC-101-20x50 | 20 x 50 μL | $ 165.00 | |
| CC-101-10x100 | 10 x 100 μL | $ 180.00 | |
| CC-101-15x100 | 15 x 100 μL | $ 242.00 |
Description
GoldBio’s GB5-alpha™ Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.
Product Specifications
Competent cell type: Chemically Competent
Equivalent to: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes
BL21 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-102-5x50 | 5 x 50 μL | $ 59.00 | |
| CC-102-10x50 | 10 x 50 μL | $ 105.00 | |
| CC-102-15x50 | 15 x 50 μL | $ 139.00 |
Description
GoldBio’s BL21 Chemically Competent E. coli cells are a good, universal strain of cell that is gives great transformation for protein expression and induction, and is especially useful for non-T7 vectors protein expression systems. BL21 chemically competent cells feature a widely used host background, and are deficient in both lon (1) and ompT proteases. In addition, BL21 Chemically Competent E. coli cells are resistant to phage T1 (fhuA2).
Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes
BL21 (DE3) Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-103-5x50 | 5 x 50 μL | $ 59.00 | |
| CC-103-10x50 | 10 x 50 μL | $ 105.00 | |
| CC-103-15x50 | 15 x 50 μL | $ 139.00 | |
| CC-103-20x50 | 20 x 50 μL | $ 165.00 |
Description
GoldBio’s BL21 (DE3) Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as transformation and routine protein expression. BL21 (DE3) chemically competent cells feature a widely used host background, a T7 expression strain, and are deficient in both lon (1) and ompT proteases. In addition, BL21 (DE3) cells are resistant to phage T1 (fhuA2) and are B strain.
Product Specifications
Competent cell type: Chemically Competent
Derivative of: BL21 (DE3)
Species: E. coli
Transformation efficiency: ≥1 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes
DL39 (DE3) Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-104-5x50 | 5 x 50 μL | $ 59.00 | |
| CC-104-10x50 | 10 x 50 μL | $ 105.00 |
Description
GoldBio’s DL39 (DE3) Chemically Competent E. coli cells are engineered with a T7 expression system to transform and express proteins in order to label residues such as phenylalanine or leucine. DL39 (DE3) Chemically Competent E. coli cells are deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases and are auxotrophic for aspartic acid, isoleucine, leucine, phenylananine, tyrosine, and valine residues.
Product Specifications
Competent cell type: Chemically Competent
Derivative of: DL39
Species: E. coli
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
HB101 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-150-5x50 | 5 x 50 µl | $ 59.00 | |
| CC-150-10x50 | 10 x 50 µl | $ 105.00 |
Description
GoldBio’s HB101 chemically competent E. coli cells allow you to obtain high transformation efficiency in applications such cloning and sub-cloning. Our E. coli HB101 proceeds from the K12 x B hybrid strain. HB101 strain also contains the recA13 mutation useful in the insert stability and minimization of recombination. E. coli HB101 also has the hsdS20(rB-mB-) genotype that prevents the cleavage of cloned DNA by endogenous restriction enzymes.
Product Specifications
Competent cell type: Chemically competent
Derivative of: HB101
Species: E. coli
Transformation efficiency: ≥7 x 106 cfu/µg pUC19 DNA
Blue/white screening: No
JM109 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-114-5x50 | 5 x 50 µl | $ 59.00 | |
| CC-114-10x50 | 10 x 50 µl | $ 105.00 |
Description
GoldBio’s JM109 Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, library construction, cloning, and subcloning. JM109 is a K strain bacterium that carries multiple features including the lacIqZΔM15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol as well as the hsdR17 genotype which prevents endogenous endonucleases from cleaving DNA.
Product Specifications
Competent cell type: Chemically competent
Derivative of: JM109
Species: E. coli
Format: Tubes
Transformation efficiency: ≥6 x 105 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
| Catalog ID | CC-114 |
|---|---|
| Storage/Handling | Store Competent Cells at -80°C. |
RR1 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-113-5x50 | 5 x 50 µl | $ 59.00 | |
| CC-113-10x50 | 10 x 50 µl | $ 105.00 |
Description
GoldBio’s RR1 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli RR1 is a recA+ derivative of the HB101 strain and can be more useful than HB101 if a recA+ background is required.
Product Specifications
Competent cell type: Chemically competent
Derivative of: RR1
Species: E. coli
Format: Tubes
Transformation efficiency: ≥3 x 107 cfu/µg pUC19 DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-113 |
|---|---|
| Storage/Handling | Store Competent Cells at -80°C. |
GoldBio's Agrobacterium electrocompetent cells are manufactured in our lab under our strict quality control standards, ensuring researchers the highest level of purity and consistency. Our Agrobacterium cells are verified in our labs to have a real efficiency up to ≥1 x 107 cfu/μg DNA. GoldBio's consistent reliability and quality give researchers an assurance and peace of mind, knowing that they are working with our trademarked proven and published products.
AGL-1 Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-208-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-208-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-208-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s AGL-1 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted in the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥5 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
AGL-1 (pSoup) Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-218-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-218-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-218-15x50 | 15 x 50 μL | $ 485.00 |
Description
AGL-1 (pSoup) electrocompetent Agrobacterium cells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-type Agrobacterium strain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, helping to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
(pSoup)
Transformation efficiency: ≥2 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
AGL-1 (pSoup-P19) Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-228-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-228-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-228-15x50 | 15 x 50 μL | $ 485.00 |
Description
AGL-1 (pSoup-P19) electrocompetent Agrobacterium cells are a highly efficient system for genetic transformation in various plant species. This strain is derived from the C58 RecA-type Agrobacterium strain and carries the rifampicin, carbenicillin, and streptomycin resistance genes for selection purposes. The Ti plasmid pTiBo542DT-DNA used in this system has a deleted T-DNA region, and instead contains a binary vector that restores the T-DNA binary system function.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: AGL-1
(pSoup-P19)
Transformation efficiency: ≥1 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-207-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-207-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-207-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥2 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup) Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-217-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-217-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-217-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s GV3101 Agrobacterium electrocompetent cells are a powerful tool for high efficiency transformation of plants. The GV3101 strain is optimized for cDNA and gDNA library construction, and possesses resistance to rifampicin, gentamicin, and tetracycline. The T-DNA region of the GV3101 Ti plasmid has been deleted, but restoration of the binary vector T-region can fully enable T-DNA binary system function.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101 (pSoup)
Transformation efficiency: ≥8 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup-P19) Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-227-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-227-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-227-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s GV3101 electrocompetent Agrobacterium cells are a highly efficient tool for genetic transformation of dicotyledonous and monocotyledonous plant species, such as Arabidopsis thaliana, tobacco, potato, and corn. These cells carry the nopaline-type Ti plasmid pMP90 (pTiC58DT-DNA) that contains the vir genes required for the transfer and integration of T-DNA into the plant genome. Additionally, the cells are resistant to rifampicin, gentamicin, and tetracycline, which allows for selection of transformed cells.
The pSoup plasmid carried by the cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes. These combined properties make GV3101 electrocompetent Agrobacterium cells an ideal choice for cDNA or gDNA library construction and the generation of transgenic plants with desirable traits.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101 (pSoup-P19)
Transformation efficiency: ≥1 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-225-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-225-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-225-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 (pSoup) Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-235-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-235-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-235-15x50 | 15 x 50 μL | $ 485.00 |
Description
EHA105 (pSoup) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥4 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 (pSoup-P19) Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-245-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-245-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-245-15x50 | 15 x 50 μL | $ 485.00 |
Description
EHA105 (pSoup-P19) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥3 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
LBA4404 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-220-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-220-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-220-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
C58C1 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-240-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-240-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-240-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s C58C1 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GoldBio Agrobacterium chemically competent cells are an attractive, simple and affordable option for researchers. All of our chemically competent Agrobacterium cell lines are manufactured in our lab under our strict quality control standards and are verified in our labs to have a real efficiency up to ≥1 x 105 cfu/μg DNA. We personally test all of our cell lines in our lab to make sure researchers can have the highest degree of confidence in our products and the greatest success in their experiments.
AGL-1 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-106-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-106-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-106-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s AGL-1 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our AGL-1 strain harbors the C58 chromosomal backbone with an insertion mutation in its recA recombination gene. This mutation stabilizes recombinant plasmids. AGL-1 also has rifampicin and carbenicillin resistance genes in the genome useful for selection. A functional T-DNA binary system can be built using our AGL-1 strains as the T-DNA region has been deleted from the Ti plasmid pTiBO542 and instead it has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant S genome. Therefore, our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, maize, and other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: AGL-1
Transformation efficiency: ≥4 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
AGL-1 (pSoup) Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-116-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-116-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-116-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s AGL-1 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.
The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the AGL-1 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: AGL-1 (pSoup)
Transformation efficiency: ≥3 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
AGL-1 (pSoup-P19) Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-126-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-126-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-126-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s AGL-1 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, carbenicillin, and streptomycin, providing versatility in selection and antibiotic resistance. The Ti plasmid pTiBo542DT-DNA has been engineered to have the T-DNA region deleted, which can be complemented with a binary vector containing the missing T-region to restore the T-DNA binary system function. This feature enables efficient and precise transformation of plant genomes.
The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes and increasing the range of applications for the AGL-1 (pSoup-P19) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: AGL-1 (pSoup-P19)
Transformation efficiency: ≥2 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-105-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-105-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-105-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s GV3101 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥6 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup) Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-115-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-115-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-115-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s GV3101 Agrobacterium chemically competent cells are a powerful tool for high efficiency transformation of plants. The GV3101 strain is optimized for cDNA and gDNA library construction, and possesses resistance to rifampicin, gentamicin, and tetracycline. The T-DNA region of the GV3101 Ti plasmid has been deleted, but restoration of the binary vector T-region can fully enable T-DNA binary system function.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101 (pSoup)
Transformation efficiency: ≥1.5 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup-P19) Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-125-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-125-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-125-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s GV3101 competent Agrobacterium cells are a highly efficient tool for genetic transformation of dicotyledonous and monocotyledonous plant species, such as Arabidopsis thaliana, tobacco, potato, and corn. These cells carry the nopaline-type Ti plasmid pMP90 (pTiC58DT-DNA) that contains the vir genes required for the transfer and integration of T-DNA into the plant genome. Additionally, the cells are resistant to rifampicin, gentamicin, and tetracycline, which allows for selection of transformed cells.
The pSoup plasmid carried by the cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes. These combined properties make GV3101 competent Agrobacterium cells an ideal choice for cDNA or gDNA library construction and the generation of transgenic plants with desirable traits.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101
(pSoup-P19)
Transformation efficiency: ≥3 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-108-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-108-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-108-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥5 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 (pSoup) Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-118-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-118-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-118-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s EHA105 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.
The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.
GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥5 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 (pSoup-P19) Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-128-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-128-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-128-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s EHA105 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.
The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.
GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥2 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
LBA4404 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-107-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-107-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-107-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
C58C1 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-109-5x50 | 5 x 50 μL | $ 189.00 | |
| CC-109-10x50 | 10 x 50 μL | $ 335.00 | |
| CC-109-15x50 | 15 x 50 μL | $ 449.00 |
Description
GoldBio’s C58C1 Chemically Competent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 105 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GoldBio's competent cell recovery medium is a specially formulated universal recovery medium that can be used for any of our cell lines, E. coli or Agrobacterium. Our YEM media is a complex media formulation that provides optimal and stress free growing conditions for cultures of both Agrobacterium and Rhizobia.
Competent Cell Recovery Medium
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-300-A | 6 x 12 ml | $ 80.00 |
Description
GoldBio’s Universal Competent Cell Recovery Medium is specifically formulated for optimal competent cell transformations of every kind of cell, including E. coli and Agrobacterium.
YEM Media
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| Y-100-500 | 500 ml | $ 105.00 |
Description
Yeast Extract Mannitol (YEM) media is often used for the growth of Agrobacterium as well as Rhizobium bacterial strains. Mannitol is used as a sugar substitute to provide a carbon source while the yeast extract provides the nitrogen for bacterial growth.
YEM media is a complex media formulation that provides optimal and stress free growing conditions for cultures of both Agrobacterium and Rhizobia.
pUC19 Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-550-10 | 10 μg | $ 25.00 |
Description
pUC19 Vector Plasmid is a compact, high-copy cloning vector with Ampicillin resistance and a versatile tool in molecular biology research. pUC19 has 2686 base pairs which harbor a 54 base-pair multiple cloning site (MCS) for efficient cloning with 13 unique restriction enzyme sites, offering flexibility and compatibility with a variety of experimental setups. It is identical to pUC18, except that their MCS are oriented opposite of each other.
pCAMBIA1391z Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-590-10 | 10 μg | $ 199.00 |
Description
pCambia1391Z is a reliable vector plasmid derived from pPZP and is a robust tool for genetic manipulation in plants. Versatile and stable, pCambia1391Z adapts to a wide array of plant species, maintaining the integrity of inserted DNA throughout transformation and regeneration. Designed specifically for promoter testing in plants, this vector harbors a promoterless version of gusA for tracking and visualizing transformed cells. It is additionally coupled with a catalase intron downstream of a truncated lacZ gene, enabling blue/white screening in suitable E. coli host strains.
pBR325 Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-595-10 | 10 μg | $ 25.00 |
Description
The pBR325 plasmid is a classic cloning vector used in molecular biology. It is derived from the pBR322 plasmid, which itself is one of the first and most widely used plasmids for cloning purposes.
pACYC Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-555-10 | 10 μg | $ 25.00 |
Description
The pACYC plasmid series is another set of commonly used cloning vectors in molecular biology and biotechnology. PACYC184 has a p15A origin of replication with chloramphenicol and tetracycline resistance genes. Unlike the pUC or pBR325 vectors, pACYC184 plasmids are characterized by a low copy number. This means fewer copies of the plasmid are present in each bacterial cell, which can be advantageous for cloning and expressing genes that are toxic to the host cells when present in high quantities. pACYC184 has a small size of 4245 bp and is suitable for cloning small to medium-sized DNA fragments. PACYC plasmids are especially useful in experiments where multiple plasmids are required within the same cell, or when cloning genes that are deleterious to host cells at high copy numbers.
pSoup Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-520-1 | 1 µg | $ 195.00 |
Description
The pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. pSoup plasmids are instrumental in helping with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
pSoup-P19 Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-525-1 | 1 μg | $ 195.00 |
Description
The pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. The combination of these features in the pSoup-P19 plasmid helps to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
