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ProBlock™ Gold Yeast/Fungi protease inhibitor cocktail contains optimized concentrations of various yeast and fungal protease inhibitors, which provide excellent inhibition of protease activities during protein purification from yeast or fungi. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for yeast or fungal proteases, such as aspartic proteases. At 1X concentration, ProBlock™ Gold Yeast/Fungi inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca²⁺, Mg²⁺ or Mn²⁺) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Yeast/Fungi is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

Y-β-D-GLC is a chromogenic substrate for β-D-glucosidase, yielding a blue precipitate upon cleavage.

The Yeast Lysis Buffer Kit is useful for extraction of soluble proteins from yeast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

This kit is provided with an optional protocol to make spheroplast and remove lytic enzyme Zymolyase®, prior to lysis and extraction of yeast proteins. Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer.

The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works with both gram-positive and gram-negative bacteria. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.

This kit is suitable for processing 100 x 50 µl yeast cell pellet preparations. Yeast Lysis Buffer is also available separately for further downstream processing.

The Yeast Spheroplast Lysis Buffer is useful for extraction of soluble proteins from spheroplast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer. The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works well with both gram-positive and gram-negative bacteria.

The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.