Introduction to Phosphatases and Phosphatase Inhibitor Cocktails

by Pallabi Roy Chakravarty, Ph.D.

How to Make an IPTG Stock Solution
Making IPTG stock solution involves weighing out IPTG powder, pouring it into a conical tube or cylinder, and adding deionized water to the desired volume....
IPTG Induction vs Auto-Induction
IPTG and auto-induction are two ways to induce protein expression in bacteria. They work similarly, but have different trade-offs in terms of convenience. While IPTG...
Optimizing Protein Expression with IPTG Induction
The final concentration of IPTG used for induction varies from 0.1 to 1.0 mM, with 0.5 or 1.0 mM most frequently used. For proteins with...
What Is a His-Tag?
A His-tag is a stretch of 6-10 histidine amino acids in a row that is used for affinity purification, protein detection, and biochemical assays. His-tags...