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Materials and Methods
3 Solubility Tags for Protein Affinity Purification
Let’s imagine that you are trying to express and purify a new protein. You attached a his-tag onto y...
E. coli Homologous Recombination: How It Works and Influences on Cloning
Homologous recombination is the exchange of sequences that occurs between two similar (homologous)...
What are E. coli Expression Strains?
E. coli expression strains, BL21(DE3) and DL39 for example, are used for recombinant RNA/protein exp...
Basic and Advanced Fluorescence Microscopy Techniques
Fluorescence microscopy spans from basic epifluorescence/widefield microscopy to advanced techniques...
10 Common Techniques For Nucleic Acid Research
There are several experimental techniques to detect and quantify nucleic acids in your sample. These...
Introduction to Phosphatases and Phosphatase Inhibitor Cocktails
Four Primary Steps and Tools for NGS Data Analyses
What do you do with millions of data? It is a recurrent question in sequencing projects. Analyzing N...
Chemiluminescent Detection Assay Using Luminol - Crash Course
A chemiluminescent assay is a type of detection assay, or detection experiment for a specific molecu...
Troubleshooting DNA Ladders
Troubleshooting issues with DNA ladders begins with determining the issue, such as smeared bands, fa...