Phenylmethylsulfonyl fluoride (PMSF)
PMSF is a serine protease inhibitor that irreversibly inhibits serine proteases by sulfonylation of the serine residue in the active site of the protease. It is widely used in protocols for the isolation of proteins.
PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C. The half-life of a 20mM aqueous solution of PMSF is ~35 minutes at pH 8.0. This short half-life means that aqueous solutions of PMSF can be safely discarded after they have been rendered alkaline (pH >8.6) and stored for several hours at room temperature.
Phenylmethylsulfonyl fluoride; PMSF
MOLECULAR BIOLOGY GRADE
MW: 174.19 g/mol
White to slightly off white cyrstalline powder
Storage/Handling: Store desiccated at 4°C.
Soluble in DMSO or 100% Ethanol.
Protect from light. Hygroscopic.
PubChem Chemical ID: 4784
Note: To enable us to avoid hazardous shipping fees, U.S. domestic ground
shipments of PMSF will be shipped as a limited quantity exception for the
25 g, 50 g, and 100 g pack sizes. In some instances GoldBio may
ship 50 g as 2 x 25 g or 100 g as 4 x 25 g to qualify for a small
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store desiccated at 4°C. Protect from light.|
|Item Class||8 (6.1)|
PMSF Stock Solution and suggested concentration in experiments.
A protocol to trigger autophagy in yeast by Cebollero and Gonzalez (2006).
Preparation of Proteins for Western Blotting of Monolayer Cells
Protease inhibitor cocktail recipe to use during mammalian cell lysis
Use of RIPA Buffer to prepare cell lysates for protein assays, protein purification and other downstream applications.
Sample Certificate of Analysis - PMSF
COA for example only. Actual tests may differ.