DTT (Dithiothreitol) (> 99% pure) Protease free

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Product Description

Dithiothreitol (DTT) is the common name of the more popular of the two Cleland’s Reagents (the other being Dithioerythritol or DTE). DTT is a powerful reducing agent that forms a stable six-membered ring with an internal disulfide bond which is resistant to oxidation. DTT is often used for the following: reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl) cystamine to break apart the matrix of a polyacrylamide gel, the reduction of disulfide bonds in proteins, the prevention of those bonds from forming between cysteine residues and lastly, DTT is often used to reduce thiolated DNA in order to minimize dimerization.

DTT is a nearly 7-fold stronger reduction agent than βME (β-mercaptoethanol) and has a less offensive odor and is less toxic.

Product Specifications:
DTT, DL-Dithiothreitol (Clelands Reagent),
(2 S,3S)-1,4-bis(sulfanyl)butane-2,3-diol,
D- threo-1,4-dimercapto-2,3-butanediol

>99% pure

PROTEASE FREE

MOLECULAR BIOLOGY GRADE

Formula: C4H10O2S2

MW: 154.25 g/mol

Storage/Handling: Store desiccated at -20°C. Solutions should be prepared fresh, daily.

PubChem Chemical ID: 19001

N.B.
During the summer, GoldBio may require shipment by Next Day Air to avoid high temperatures that may adversely affect DTT quality.

Product Specifications

Catalog ID DTT
CAS # 27565-41-9 / 3483-12-3
MW 154.25 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.

Technical Documentation

P Protocol
O Other
C Certificate of Analysis
S Safety Data Sheet
P
DTT Stock Solution

1M DTT Stock Solution
P
Gus Gene Assay in Transformed Plant Tissue

Procedures for GUS gene expression, fluorimetric and fluorigenic assays by Dr. Paul Bottino, Plant Molecular Genetics (retired), University of Maryland.
P
In Gel Digestion and Extraction of Proteins Protocol

In Gel Digestion of proteins - including destaining, alkylation, digestion and recovery.
P
IPTG Induction and Extraction of Proteins Protocol

IPTG Induction & Extraction of Proteins - by Arur and Nayak. Schedl Lab, Wash U. St. Louis
P
Preparation of Mucoid Specimens utilizing DTT

A ThinPrep Non-GYN protocol from HOLOGIC® for respiratory and gastrointestinal specimens.
P
Protein Digestion utilizing Trypsin

Digestion of Proteins utilizing Trypsin Protocol
C
Sample COA - DTT

Sample Certificate of Analysis - DTT
COA for example only. Actual tests may differ.
S
SDS (MSDS) - DTT

Safety Data Sheet

Citations

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