Reducing Agents (part 1 of 4) - Dithiothreitol
There are a number of reducing agents that are available at Gold Bio: DTT (dithiothreitol), DTE (dit...
Dithiothreitol (DTT) is the common name of the more popular of the two Cleland’s Reagents (the other being Dithioerythritol or DTE). DTT is a powerful reducing agent that forms a stable six-membered ring with an internal disulfide bond which is resistant to oxidation. DTT is often used for the following: reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl) cystamine to break apart the matrix of a polyacrylamide gel, the reduction of disulfide bonds in proteins, the prevention of those bonds from forming between cysteine residues and lastly, DTT is often used to reduce thiolated DNA in order to minimize dimerization.
DTT is a nearly 7-fold stronger reduction agent than βME (β-mercaptoethanol) and has a less offensive odor and is less toxic.
Product Specifications:
DTT, DL-Dithiothreitol (Clelands Reagent),
(2
S,3S)-1,4-bis(sulfanyl)butane-2,3-diol,
D-
threo-1,4-dimercapto-2,3-butanediol
>99% pure
PROTEASE FREE
MOLECULAR BIOLOGY GRADE
Formula: C4H10O2S2
MW: 154.25 g/mol
Storage/Handling: Store desiccated at -20°C. Solutions should be prepared fresh, daily.
PubChem Chemical ID: 19001
N.B.
During the summer, GoldBio may require shipment by Next Day Air to avoid high temperatures that may adversely affect DTT quality.
Catalog ID | DTT |
---|---|
CAS # | 27565-41-9 / 3483-12-3 |
MW | 154.25 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store desiccated at -20°C. |
There are a number of reducing agents that are available at Gold Bio: DTT (dithiothreitol), DTE (dit...
Previously, we’ve been discussing the function of various reducing agents. There are a number of red...
Previously, we’ve been discussing the function of various reducing agents. There are a number of red...
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