DTT (Dithiothreitol) (> 99% pure) Protease free

Dithiothreitol (DTT) is the common name of the more popular of the two Cleland’s Reagents (the other being Dithioerythritol or DTE). DTT is a powerful reducing agent that forms a stable six-membered ring with an internal disulfide bond which is resistant to oxidation. DTT is often used for the following: reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl) cystamine to break apart the matrix of a polyacrylamide gel, the reduction of disulfide bonds in proteins, the prevention of those bonds from forming between cysteine residues and lastly, DTT is often used to reduce thiolated DNA in order to minimize dimerization.

DTT is a nearly 7-fold stronger reduction agent than βME (β-mercaptoethanol) and has a less offensive odor and is less toxic.

N.B.
During the summer (between 5/1 and 9/30), GoldBio may require shipment by Next Day Air with blue ice in order to avoid high temperatures that may adversely affect DTT quality.

Applications

• PROTEASE FREE Oxidizing agent
• Reducing agent for polyacrylamide gels
• Denaturation of protein and peptide disulfide bond
• Sperm cell lysis
• Stabilizer for specific enzymes; i.e. Taq polymerase and restriction digest enzymes
• Protects from oxidation damage in lysis buffers

Storage/Handling

Store desiccated at -20°C. Solutions should be prepared fresh, daily.

Working Concentration

DTT is commonly stored in a 1M stock solution for up to 1 year at -20°C.
Working concentrations are typically 1-10mM and should be remade daily.