TCEP HCl

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Description

TCEP (Tris (2-Carboxyethyl) phosphine Hydrochloride) is an odorless reducing agent suitable for protein applications and in the preparation for electrophoresis. TCEP is water soluble, is stable in a variety of solutions and is particularly useful in the labeling of cysteine residues with maleimides, preventing the cysteines from forming disulfide bonds.

TCEP 50 g and 100 g ships as multiple 25 g units to qualify for a small quantity exception to hazardous fees.
Due to hazardous goods restrictions, this product cannot ship by ground shipping to Canada or Mexico.

Benefits

  • PROTEASE FREE Oxidizing agent
  • TCEP-HCl is stable in air.
  • TCEP-HCl is odorless so reactions can be carried out on the bench instead of the fume hood.
  • TCEP-HCl is efficient and can be used to reduce disulfide bonds at low concentrations.
  • TCEP-HCl is selective for disulfides and does not react with other protein side chains.
  • TCEP-HCl may be used as a substitute for DTT at a final concentration of 50mM.

Storage/Handling

Store desiccated at -20°C.

Working Concentration

TCEP is commonly stored in a 0.5M stock solution for up to 3 months at -20°C.
Working concentrations are typically around 5-50mM and should be remade daily.

TCEP HCl

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Catalog Number:
TCEP1
CAS Number:
51805-45-9
$33.00

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    Description

    TCEP (Tris (2-Carboxyethyl) phosphine Hydrochloride) is an odorless reducing agent suitable for protein applications and in the preparation for electrophoresis. TCEP is water soluble, is stable in a variety of solutions and is particularly useful in the labeling of cysteine residues with maleimides, preventing the cysteines from forming disulfide bonds.

    TCEP 50 g and 100 g ships as multiple 25 g units to qualify for a small quantity exception to hazardous fees.
    Due to hazardous goods restrictions, this product cannot ship by ground shipping to Canada or Mexico.

    Benefits

    • PROTEASE FREE Oxidizing agent
    • TCEP-HCl is stable in air.
    • TCEP-HCl is odorless so reactions can be carried out on the bench instead of the fume hood.
    • TCEP-HCl is efficient and can be used to reduce disulfide bonds at low concentrations.
    • TCEP-HCl is selective for disulfides and does not react with other protein side chains.
    • TCEP-HCl may be used as a substitute for DTT at a final concentration of 50mM.

    Storage/Handling

    Store desiccated at -20°C.

    Working Concentration

    TCEP is commonly stored in a 0.5M stock solution for up to 3 months at -20°C.
    Working concentrations are typically around 5-50mM and should be remade daily.

    Product Specifications

    Catalog ID: TCEP
    CAS #: 51805-45-9
    Formula: C9H15O6P • HCl
    MW: 286.65 g/mol
    Grade: Molecular Biology Grade
    Storage/handling: Store desiccated at -20°C.
    UN number: 3261
    item class: 8
    group number: II
    PubChem Chemical ID: 2734570

    CITATIONS

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