Protein Purification
Separating proteins from cellular components is critical when trying to determine their overall structure and function. GoldBio carries products designed for the whole range of chromatography applications, from gel chromatography to more targeted applications like affinity, ion exchange or polyhistidine-tag binding. When it comes to purification, the higher the quality of materials, the better the results. GoldBio agaroses come in different densities, with high throughput beads designed for industrial applications. Our Cobalt and Nickel agarose beads are perfect for your his-tag recombinant protein recovery, and our Ultra HBCTM streptavidin resin has superior coupling chemistry and higher binding performance than other vendors. GoldBio offers quality products, at affordable prices.
Cobalt Agarose Beads (High Density)
Catalog ID | Size | Pricing | |
---|---|---|---|
H-310-5 | 5 mL | $ 65.00 | |
H-310-10 | 10 mL | $ 105.00 | |
H-310-25 | 25 mL | $ 229.00 | |
H-310-50 | 50 mL | $ 385.00 | |
H-310-100 | 100 mL | $ 675.00 | |
H-310-500 | 500 mL | $ 2,755.00 |
Description
Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.
Product Specifications
Catalog ID | H-310 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Cobalt HTC Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
R-203-25 | 25 mL | $ 306.00 | |
R-203-100 | 100 mL | $ 772.00 | |
R-203-500 | 500 mL | $ 2,965.00 |
Description
Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).
Product Specifications
Catalog ID | R-203 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Glutathione Agarose Resin
Catalog ID | Size | Pricing | |
---|---|---|---|
G-250-5 | 5 mL | $ 105.00 | |
G-250-10 | 10 mL | $ 195.00 | |
G-250-50 | 50 mL | $ 739.00 | |
G-250-100 | 100 mL | $ 1,149.00 |
Description
Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.
Product Specifications
Catalog ID | G-250 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Imidazole - 3M Recrystallized Solution, pH 6.0
Catalog ID | Size | Pricing | |
---|---|---|---|
I-901-25 | 25 mL | $ 89.00 | |
I-901-50 | 50 mL | $ 135.00 | |
I-901-100 | 100 mL | $ 205.00 | |
I-901-250 | 250 mL | $ 459.00 |
Description
Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.
Product Specifications
Catalog ID | I-901 |
---|---|
CAS # | 288-32-4 |
MW | 68.08 g/mol |
Storage/Handling | Store at 4°C. |
Imidazole, Recrystallized
Catalog ID | Size | Pricing | |
---|---|---|---|
I-902-25 | 25 g | $ 79.00 | |
I-902-50 | 50 g | $ 125.00 | |
I-902-100 | 100 g | $ 205.00 | |
I-902-500 | 500 g | $ 609.00 |
Description
Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.
Product Specifications
Catalog ID | I-902 |
---|---|
CAS # | 288-32-4 |
MW | 68.08 g/mol |
Storage/Handling | Store at room temperature. |
UN Number | 3262 |
Item Class | 8 |
Group Number | III |
NDSB-201 (Non-detergent sulfobetaine)
Catalog ID | Size | Pricing | |
---|---|---|---|
N-201-25 | 25 g | $ 29.00 | |
N-201-50 | 50 g | $ 35.00 | |
N-201-100 | 100 g | $ 65.00 | |
N-201-250 | 250 g | $ 119.00 |
Description
NDSB-201 is a protein specific additive that can be used to help solubilize and stabilize proteins at non-denaturing concentrations while preventing protein aggregation.
NDSB-201 is a member of a group of nondetergent sulfobetaines. These zwitterionic compounds can reduce aggregation and aid in refolding and renaturing of proteins found in bacterial expression systems and in inclusion bodies. Unlike detergents such as CHAPS, NDSBs are nondenaturing, even at higher concentrations and have been shown to increase the yield efficiency of membrane and nuclear proteins, among others.
Product Specifications
Catalog ID | N-201 |
---|---|
CAS # | 15471-17-7 |
MW | 201.24 g/mol |
Storage/Handling | Store desiccated at room temperature. |
Nickel Agarose Beads (High Density)
Catalog ID | Size | Pricing | |
---|---|---|---|
H-320-5 | 5 mL | $ 59.00 | |
H-320-10 | 10 mL | $ 99.00 | |
H-320-25 | 25 mL | $ 219.00 | |
H-320-50 | 50 mL | $ 365.00 | |
H-320-100 | 100 mL | $ 649.00 | |
H-320-500 | 500 mL | $ 2,649.00 |
Description
Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.
IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.
Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.
Product Specifications
Catalog ID | H-320 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Nickel HTC Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
R-202-25 | 25 mL | $ 306.00 | |
R-202-100 | 100 mL | $ 772.00 | |
R-202-500 | 500 mL | $ 2,964.00 |
Description
Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).
HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.
Product Specifications
Catalog ID | R-202 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Nickel NTA Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
H-350-5 | 5 mL | $ 75.00 | |
H-350-10 | 10 mL | $ 119.00 | |
H-350-25 | 25 mL | $ 255.00 | |
H-350-50 | 50 mL | $ 439.00 | |
H-350-100 | 100 mL | $ 825.00 | |
H-350-500 | 500 mL | $ 3,625.00 |
Description
Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.
NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.
Product Specifications
Catalog ID | H-350 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Nickel NTA Magnetic Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
H-351-2 | 2 mL | $ 114.00 | |
H-351-5 | 5 mL | $ 255.00 | |
H-351-10 | 10 mL | $ 460.00 |
Description
Nickel NTA Magnetic Agarose Beads are a rapid and easy small scale purification of histidine-tagged proteins. This resin consists of magnetic agarose derivatized with Nitrilotriacetic (NTA) and provides good properties working in native or denaturing conditions. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.
NTA cross-linked resins consist of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent which provides a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.
Product Specifications
Catalog ID | H-351 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Protein A Agarose Resin (Pharmaceutical grade for research)
Catalog ID | Size | Pricing | |
---|---|---|---|
P-400-5 | 5 mL | $ 195.00 | |
P-400-25 | 25 mL | $ 755.00 | |
P-400-50 | 50 mL | $ 1,385.00 | |
P-400-100 | 100 mL | $ 2,349.00 |
Description
Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.
Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).
Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).
Product Specifications
Catalog ID | P-400 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Protein G HTC Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
P-430-5 | 5 mL | $ 375.00 | |
P-430-25 | 25 mL | $ 1,306.00 |
Description
Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.
GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.
Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.
Product Specifications
Catalog ID | P-430 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Streptavidin Agarose Resin
Catalog ID | Size | Pricing | |
---|---|---|---|
S-105-1 | 1 mL | $ 79.00 | |
S-105-5 | 5 mL | $ 289.00 | |
S-105-10 | 10 mL | $ 505.00 |
Description
Streptavidin Agarose Resin is a high affinity biotin-binding chromatography resin with streptavidin covalently bound to 6% crosslinked agarose beads and a biotin binding capacity of >120 nmol/ml of gel. Immobilized Streptavidin resin is suitable for gravity flow columns, spin columns, and FPLC methods. Immobilized Streptavidin can be used for affinity chromatography purifications by separating biotinylated proteins from nonbiotinylated proteins and for affinity purification of antigens when used with biotinylated antibodies.
Product Specifications
Catalog ID | S-105 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |