Imidazole, Recrystallized

Request Lot Specific COA

Citation Search Tool

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins. Imidazole is also commonly used as a buffer for enzymatic reactions and has a buffering pH range of 6.2 - 7.8.

Recrystallized Imidazole from GoldBio is specially purified Imidazole designed for histidine tagged protein purification. We purify the Imidazole at Gold Biotechnology’s labs and go through multiple purification steps including ultrafiltration and recrystallization to obtain the most purified Imidazole on the market. The final Imidazole product is tested in our labs for elution of tagged protein from Ni columns.

Product Specifications:
Sterile filtered and recrystallized Imidazole;
Glyoxaline;
1,3-diazacyclopenta-2,4-diene

Molecular Biology Grade
Protease Free

Formula: C3H4N2

MW: 68.08 g/mol

Ships in 25 g units

PubChem Chemical ID: 795

Imidazole, Recrystallized

View Sizes & Pricing

Catalog Number:
I-902-25
CAS Number:
288-32-4
$82.00

Availability:
In stock
Shipping:
Shipping calculated at checkout

    Description

    Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins. Imidazole is also commonly used as a buffer for enzymatic reactions and has a buffering pH range of 6.2 - 7.8.

    Recrystallized Imidazole from GoldBio is specially purified Imidazole designed for histidine tagged protein purification. We purify the Imidazole at Gold Biotechnology’s labs and go through multiple purification steps including ultrafiltration and recrystallization to obtain the most purified Imidazole on the market. The final Imidazole product is tested in our labs for elution of tagged protein from Ni columns.

    Product Specifications:
    Sterile filtered and recrystallized Imidazole;
    Glyoxaline;
    1,3-diazacyclopenta-2,4-diene

    Molecular Biology Grade
    Protease Free

    Formula: C3H4N2

    MW: 68.08 g/mol

    Ships in 25 g units

    PubChem Chemical ID: 795

    Product Specifications

    Catalog ID: I-902
    CAS #: 288-32-4
    MW: 68.08 g/mol
    Storage/handling: Store at room temperature.
    UN number: 3263
    item class: 8
    group number: III

    CITATIONS

    Featured Articles

    • what's a his-tag thumbnail image

      What Is a His-Tag?

      A His-tag is a stretch of 6-10 histidine amino acids in a row that is used for affinity purification, protein detection, and biochemical assays. His-tags...

      Read more

    • How to Optimize Buffer Components for Nickel Agarose Beads

      How to Optimize Buffer Components for Nickel Agarose Beads

      Nickel agarose beads are compatible with a wide range of buffers. However, it is important to limit the amount of metal chelating agents, such as...

      Read more

    Login

    Forgot your password?

    Don't have an account yet?
    Create account