Imidazole - 3M Recrystallized Solution, pH 6.0
Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.
Recrystallized Imidazole from GoldBio is specially purified Imidazole specifically designed for Histidine tagged protein purification. We purify the Imidazole at Gold Biotechnology’s labs and go through multiple purification steps including ultrafiltration and recrystallization to obtain the most purified Imidazole on the market. The final Imidazole product is tested in our labs for elution of tagged protein from Ni columns.
Sterile filtered and recrystallized 3M Imidazole;
MW= 68.08 g/mol.
(20.42 g/100 mL)
Storage/Handling: Store at 4°C
PubChem Chemical ID: 795
|Storage/Handling||Store at 4°C.|
GoldBio His-Tag Column Prep Protocol
Quick and Simple His-Tag Column Prep protocol including Pouring and Packing an Agarose Column
Sample COA - I-901
Sample Certificate of Analysis - Imidazole - 3M Recrystallized Solution, pH 6.0
COA for example only. Actual tests may differ.