Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins. Imidazole is also commonly used as a buffer for enzymatic reactions and has a buffering pH range of 6.2 - 7.8.
Recrystallized Imidazole from GoldBio is specially purified Imidazole designed for histidine tagged protein purification. We purify the Imidazole at Gold Biotechnology’s labs and go through multiple purification steps including ultrafiltration and recrystallization to obtain the most purified Imidazole on the market. The final Imidazole product is tested in our labs for elution of tagged protein from Ni columns.
Sterile filtered and recrystallized Imidazole;
Molecular Biology Grade
MW: 68.08 g/mol
Ships in 25 g units
PubChem Chemical ID: 795
|Storage/Handling||Store at room temperature.|
Quick and Simple His-Tag Column Prep protocol including Pouring and Packing an Agarose Column
IPTG Induction & Extraction of Proteins - by Arur and Nayak. Schedl Lab, Wash U. St. Louis
Protocol for the use of Nickel NTA magnetic agarose beads in the purification of recombinant proteins labeled with histidine residues
Guide: Commonly used Buffers contains name, pH range, applications, composition and other characteristics of buffers commonly used in the lab.
Sample Certificate of Analysis - Imidazole, Recrystallized
COA for example only. Actual tests may differ.