T_Products

Timentin™ is a 15:1 mixture of ticarcillin and clavulanate. Ticarcillin is a penicillin ß-lactam antibiotic, which is susceptible to ß-lactamase degradation. When combined with clavulanate, a ß-lactamase inhibitor, its efficacy is greatly increased. Timentin™ exhibits high activity against gram-negative bacteria and resistant Agrobacterium species.

The Timentin™ EZ Pak™ is the fastest and easiest way to make a set amount of sterile Timentin™ solution. The kit includes pre weighed Timentin™ powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H₂O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio Timentin™ and the sterile solution is ready for tissue culture, bacterial media, or any number of uses.

Tissue Cell Lysis Buffer has been developed for extraction of total soluble protein from fresh or frozen animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue Cell Lysis Buffer. Tissue Cell Lysis Buffer reagent has been tested for use with a wide variety of animal tissues.

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.

Tumor necrosis factor (or TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK-cells, LAK-cells, astrocyte endothelial cells, smooth muscle cells and some transformed cells. TNF occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally occurring form of TNF is glycosylated, but nonglycosylated recombinant TNF has comparable biological activity. The biologically active native form of TNF is reportedly a trimer. Human and murine TNF has approximately 79% homology at the amino acid level and there is cross-reactivity between the two species. Two types of receptors for TNF, p55 and p75, have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Tumor necrosis factor (or TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK-cells, LAK-cells, astrocyte endothelial cells, smooth muscle cells and some transformed cells. TNF occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally occurring form of TNF is glycosylated, but nonglycosylated recombinant TNF has comparable biological activity. The biologically active native form of TNF is reportedly a trimer. Human and murine TNF has approximately 79% homology at the amino acid level and there is cross-reactivity between the two species. Two types of receptors for TNF, p55 and p75, have been described and virtually all cell types studied show the presence of one or both of these receptor types.

The clinical use of the potent antitumor activity of TNF has been limited by the pro-inflammatory side effects including fever, dose-limiting hypotension, hepatotoxicity, intravascular thrombosis and hemorrhage. Designing clinically applicable TNF mutants with low systemic toxicity has been an intense pharmacological interest. Human TNF variants, which bind to the murine TNF-R55 but not to the murine TNF-R75, exhibit retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF, which binds to both murine TNF receptors. Based on these results, many TNF mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro, and exhibited lower systemic toxicity in vivo.

Tumor necrosis factor (or TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK-cells, LAK-cells, astrocyte endothelial cells, smooth muscle cells and some transformed cells. TNF occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF is glycosylated, but nonglycosylated recombinant TNF has comparable biological activity. The biologically active, native form of TNF is reportedly a trimer. Human and murine TNF has approximately 79% homology at the amino acid level and there is cross-reactivity between the two species. Two types of receptors for TNF, p55 and p75, have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Tumor necrosis factor (or TNF-α), also called cachectin, is produced by neutrophils, activated lymphocytes, macrophages, NK-cells, LAK-cells, astrocyte endothelial cells, smooth muscle cells and some transformed cells. TNF can occur as a secreted, soluble form or as a membrane-anchored form, both of which are biologically active. Mouse TNF occurs as a membrane-anchored form. The naturally occurring form of TNF is glycosylated, but nonglycosylated recombinant TNF has comparable biological activity. The biologically active native form of TNF is reportedly a trimer. Human and murine TNF has approximately 79% homology at the amino acid level and there is cross-reactivity between the two species. Two types of receptors for TNF, p55 and p75, have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Tobramycin is an aminoglycoside antibiotic isolated from Streptomyces tenebrarius targeting gram-negative bacteria. It is often used to combat Pseudomonas species. It has been used to study cystic fibrosis and a variety of respiratory infections. Tobramycin sulfate is freely soluble in aqueous solution.

trans-Aconitic acid (abbreviated TAA) is the trans isomer of Aconitic acid. As an antimicrobial agent trans-Aconitic acid is used as an antileishmanial to prevent L. donovani from transforming from amastigotes to promastigotes, thus inhibiting the reproduction of the bacteria. In synthesis, TAA allows for the crosslinking of carboxylic acids to create polyvinyl alcohol films that are water-soluble. Found in range grasses at varying concentrations, it inhibits the tricarboxylic acid cycle it may be partially responsible for nutritional disorders in grazing cattle.

trans-Ferulic acid is a hydroxycinnamic acid found predominantly in plant cell wall components. Given its bioavailability it has been used in several studies with bacteria such as Pseudomonas acidovorans and fungi like Sporotrichum pulverulentum for analyzing metabolism and degradation of trans-Ferulic acid. In several different studies the inclusion of trans-ferulic acid as part of a complex was useful for stabilizing or increasing the absorption of pharmaceuticals or vitamins. It has chemical properties that make it both an antioxidant and a metabolite of eukaryotes in plants. For lab applications it is used as a matrix for proteins in MALDI mass spectrometry and as a reagent for the synthesis of vanillin, along with other cyclic molecules. It can also be used to esterify methacrylated dextran and itself can form a polymerize.

Trans-Stilbene is used in transition metal-catalyzed asymmetric epoxidation and dihydroxylation. It is also useful in making synthetic estrogens.

trans-Zeatin is a naturally occurring cytokinin plant growth regulator routinely used in plant tissue culture to induce cell division and shoot formation. trans-zeatin is freely soluble in acetic acid and water.

trans-Zeatin and other cytokinins are used in plant cell culture to stimulate plant cell division and induce shoot formation. Cytokinins have also been shown to help plants better cope with environmental stress.

Zeatin Riboside is the most active and ubiquitous form of the naturally occurring cytokinins. Cytokinins promote cell division, stimulate shoot proliferation, inhibit root formation, slow the aging process, and activate gene expression and metabolic activity in general. In mature plants, natural cytokinin hormone levels are high during maximum growth periods. Concentrations of cytokinin used for horticulture vary between 0.1 to 10 mg/L.

Trehalose is a naturally occurring disaccharide that is synthesized by cells to help retain cellular integrity under stress conditions. Trehalose functions to stabilize protein structures and to prevent proteins degradation.

Tricine is a zwitterionic buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is part of the Tris family of buffers with a useful pH range of 7.4 – 8.8. Tricine is commonly used in SDS-PAGE to separate peptides that are in the molecular weight range of 1 to 100 kDa. It is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography. Tricine was determined to be the best buffer tested for use in ATP assays involving firefly luciferase. Tricine can serve as scavenger of hydroxyl radicals; this should be taken into consideration if using tricine in the presence of an enzyme with oxidase activity. Tricine forms a complex with Cu(II). The addition of Cu2+ to a solution enables tricine to be used in the Lowry protein assay. Tricine can be used as a substitute for protocols that utilize the controlled substance barbital as a buffer.

Tridecyl b-D-maltopyranoside is a disaccharide derivative and glycoside which is applied as a non-ionic detergent and surfactant. In studies on membrane protein crystallization, Tridecyl b-D-maltopyranoside proved to be an effective detergent for maintaining protein solubility but necessitates the presence of salt. Tridecyl b-D-maltopyranoside has a longer chain length than Decyl β-D-maltopyranoside, another detergent used on membrane protein crystallization. Decyl β-D-maltopyranoside has been used in micellar electrokinetic chromatography to study enantiomer separation of drugs.

Trigonelline is an alkaloid and the product of niacin metabolism excretion. It is found in plants such as the coffee plant. In plants trigonelline is considered a diverse regulator for both cell cycle and other basic functions. Trigonelline can inhibit the Nrf2 transcription factor, rendering pancreatic cancer cells more susceptible to apoptosis. It also possesses an anti-diabetic effect when on KK-Ay when used on mice.

Trimethoprim is a bactericidal antibiotic effective against bacteria responsible for urinary tract infections, prostatitis, and cystitis. It is commonly combined with sulfamethoxazole to combat Staphylococcus aureus.