Protease Inhibitors
1,10-Phenanthroline monohydrate
Catalog ID | Size | Pricing | |
---|---|---|---|
P-980-10 | 10 g | $ 51.00 | |
P-980-25 | 25 g | $ 98.00 | |
P-980-50 | 50 g | $ 150.00 | |
P-980-100 | 100 g | $ 304.00 | |
P-980-250 | 250 g | $ 428.00 |
Description
Product Specifications
Catalog ID | P-980 |
---|---|
CAS # | 5144-89-8 |
MW | 198.22 g/mol |
Storage/Handling | Store desiccated under inert gas at room temperature. |
AEBSF
Catalog ID | Size | Pricing | |
---|---|---|---|
A-540-500 | 500 mg | $ 67.00 | |
A-540-1 | 1 g | $ 119.00 | |
A-540-2.5 | 2.5 g | $ 253.00 | |
A-540-5 | 5 g | $ 376.00 | |
A-540-10 | 10 g | $ 679.00 | |
A-540-25 | 25 g | $ 1,520.00 |
Description
AEBSF is an irreversible serine protease inhibitor. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin, kallikrein and thrombin. It inhibits the active site of the enzyme through acylation. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25 mM. Aqueous solutions are stable between pH 5-6; limited stability above pH 7.5.
Product Specifications
Catalog ID | A-540 |
---|---|
Name(s) |
AEBSF·HCl; Pefabloc SC; 4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride |
CAS # | 30827-99-7 |
Formula | C8H10FNO2S · HCl |
MW | 239.69 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
PubChem Chemical ID | 186136 |
Storage/Handling | Store at -20°C. Protect from light. |
Aprotinin
Catalog ID | Size | Pricing | |
---|---|---|---|
A-655-25 | 25 mg | $ 67.00 | |
A-655-100 | 100 mg | $ 160.00 |
Description
Aprotinin is a monomeric globular polypeptide derived from bovine lung tissue. It consists of a chain of 58 amino acids that fold into a stable, compact tertiary structure of the 'small SS-rich" type, containing 3 disulfides, a twisted β-hairpin and a C-terminal α-helix.
Aprotinin inhibits several serine proteases, specifically trypsin, chymotrypsin, plasmin and kallikrein. Its action on kallikrein leads to the inhibition of the formation of factor XIIa. Binding is reversible with most aprotinin-protease complexes dissociating at pH >10 or < 3. Aprotinin is freely soluble in water (>10 mg/ml) as well as low ionic strength aqueous buffers.
Product Specifications
Catalog ID | A-655 |
---|---|
CAS # | 9087-70-1 |
MW | 6511.44 g/mol |
Storage/Handling | Store at 4°C. |
Asunaprevir
Catalog ID | Size | Pricing | |
---|---|---|---|
A-820-10 | 10 mg | $ 329.00 | |
A-820-25 | 25 mg | $ 657.00 |
Description
Asunaprevir is a potent inhibitor of the enzyme serine protease NS3 in hepatitis C viruses (HCV) and is currently in phase III clinical trials. This enzyme’s C terminal functions as a helicase for host DNA allowing access for viral manipulation. Asunaprevir is used in tandem with ribavirin and other direct or indirect acting antiviral compounds results in a thorough eradication of HCV.
Product Specifications
Catalog ID | A-820 |
---|---|
CAS # | 630420-16-5 |
MW | 748.28 g/mol |
Storage/Handling | Store at -20°C. |
Benzamidine Hydrochloride Monohydrate
Catalog ID | Size | Pricing | |
---|---|---|---|
B-050-500 | 500 g | $ 447.00 | |
B-050-1 | 1 kg | $ 776.00 |
Description
Benzamidine hydrochloride monohydrate is an aromatic amidine. It is used in protein extractions as a strong reversible inhibitor of serine proteases such as trypsin, thrombin, plasmin and other trypsin-like proteases. Compared to aprotinin, benzamidine is considered to be just as effective in preventing the degradation of glucogon.
Product Specifications
Catalog ID | B-050 |
---|---|
CAS # | 1670-14-0 |
MW | 174.63 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store desiccated at -20°C. Protect from light. |
Bestatin
Catalog ID | Size | Pricing | |
---|---|---|---|
B-915-100 | 100 mg | $ 78.00 | |
B-915-250 | 250 mg | $ 170.00 | |
B-915-500 | 500 mg | $ 263.00 | |
B-915-1 | 1 g | $ 397.00 |
Description
Product Specifications
Catalog ID | B-915 |
---|---|
CAS # | 58970-76-6 |
MW | 308.37 g/mol |
Storage/Handling | Store at room temperature. |
Carfilzomib
Catalog ID | Size | Pricing | |
---|---|---|---|
C-915-5 | 5 mg | $ 152.00 | |
C-915-25 | 25 mg | $ 592.00 |
Description
Carfilzomib is a second generation proteasome inhibitor that has been used in oncology research.Being a derivative of epoxomicin, carfilzomib has a tetrapeptide epoxyketone structure.
The anti-cancer activity of carfilzomib on proteasomes is attributed to irreversibly binding to a 20S subunit on proteasomes. Affinity of carfilzomib for the subunit has been shown to be fairly significant, allowing carfilzomib to be effective at targeting multiple myeloma and lymphoma.
Proteasomes degrade excess or damaged proteins in both the nucleus and cytosol by cleaving hydrophobic residue peptide bonds. When proteasomes are inhibited by carfilzomib, polyubiquinated proteins accumulate. Polyubiquinated proteins will upregulate p21 synthesis, which activates cell cycle arrest and eventually apoptosis.
Carfilzomib has also been shown to have poor solubility in aqueous solutions, but greater solubility in organic solvents such as DMSO. Aqueous solubility can be enhanced by diluting carfilzomib-organic solvent solution in aqueous buffers. Using aqueous carfilzomib in biological settings should be done in dilute concentrations to limit organic solvent presence. Because carfilzomib organic-aqueous solutions only have a shelf life of approximately 24 hours, it is recommended to make small portions for immediate use.
Product Specifications
Catalog ID | C-915 |
---|---|
CAS # | 868540-17-4 |
MW | 719.91 g/mol |
Storage/Handling | Store at 4°C. |
E-64
Catalog ID | Size | Pricing | |
---|---|---|---|
E-064-5 | 5 mg | $ 102.00 | |
E-064-25 | 25 mg | $ 160.00 | |
E-064-100 | 100 mg | $ 463.00 |
Description
E-64 (trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane) irreversibly inhibits cysteine proteases without affecting other enzymes that contain cysteine residues. E-64 is capable of inhibiting bromelain, trypsin, papain, staphopain, calpain, collagenase, ficain, cathepsin B, H and L. Inhibition of serine proteases is not seen with E-64, excepting trypsin.
E-64 is usually used in a concentration range of 1 to 10μM. It is widely used for in vivo studies due to its cell permeability, low toxicity, and potency. E-64 is being investigated for the treatment of diseases caused by cysteine protease activity. It has been observed to inhibit programmed cell death and restore antigen and mitogen activity related to normal proliferative responses in T cells obtained from HIV+ donors.
Product Specifications
Catalog ID | E-064 |
---|---|
CAS # | 66701-25-5 |
MW | 357.41 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at -20°C. |
Ixazomib Citrate
Catalog ID | Size | Pricing | |
---|---|---|---|
I-350-10 | 10 mg | $ 196.00 |
Description
Ixazomib citrate is a citrate salt that is a second generation proteasome inhibitor used for anti-cancer research. In vivo research has shown ixazomib citrate to hydrolyze into its active ixazomib form which reversibly inhibits 20S proteasomes in multiple myeloma cancer cells. Ceasing this enzyme’s activity has been shown to decrease angiogenesis, induce apoptotic pathways and upregulate tumor suppressor genes.
Product Specifications
Catalog ID | I-350 |
---|---|
CAS # | 1239908-20-3 |
MW | 517.12 g/mol |
Leupeptin Hemisulfate
Catalog ID | Size | Pricing | |
---|---|---|---|
L-010-5 | 5 mg | $ 57.00 | |
L-010-25 | 25 mg | $ 109.00 | |
L-010-100 | 100 mg | $ 346.00 |
Description
Leupeptin hemisulfate (Ac-Leu-Leu-argininal) is a reversible inhibitor of serine and cysteine endopeptidases. Leupeptin hemisulfate is capable of inhibiting plasmin, trypsin, papain, kallikrein, calpain and cathepsin B. Leupeptin is usually used in a concentration range of 10 to 100μM. Leupeptin is widely used in many protease inhibitor cocktails due to its potency, low toxicity, and range of inhibition. Leupeptin is also used in a quantitative assay to measure macroautophagic flux in mammals and is being investigated for its use in treating malaria.
Product Specifications
Catalog ID | L-010 |
---|---|
CAS # | 103476-89-7 |
MW | 475.59 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at -20°C. |
Pepstatin A
Catalog ID | Size | Pricing | |
---|---|---|---|
P-020-5 | 5 mg | $ 51.00 | |
P-020-25 | 25 mg | $ 150.00 | |
P-020-100 | 100 mg | $ 428.00 |
Description
Pepstatin A is a competitive, reversible aspartic protease inhibitor and inhibits proteases such as pepsin, chyomsin, renin, HIV proteases, and cathepsins D and E. Peptstatin A is highly selective and is often used in a mixture with other enzyme inhibitors. In vitro studies have shown pepstatin A is the only aspartic acid inhibitor that exhibits inhibitory effects on HIV proteases.
Pepstatin A is effective at inhibiting most affected proteases at a concentration of 1μM, but can inhibit pepsin at the picomolar range of concentrations. It is believed to function by way of a collected-substrate inhibition mechanism. Pepstatin A may also suppress differentiation of osteoclasts by inhibiting phosphorylation of MAP kinase and inhibiting NFATc1 expression.
Product Specifications
Catalog ID | P-020 |
---|---|
CAS # | 26305-03-3 |
MW | 685.89 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Phenylmethylsulfonyl fluoride (PMSF)
Catalog ID | Size | Pricing | |
---|---|---|---|
P-470-10 | 10 g | $ 72.00 | |
P-470-25 | 25 g | $ 129.00 | |
P-470-50 | 50 g | $ 247.00 | |
P-470-100 | 100 g | $ 370.00 |
Description
PMSF is a serine protease inhibitor that irreversibly inhibits serine proteases by sulfonylation of the serine residue in the active site of the protease. It is widely used in protocols for the isolation of proteins.
PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C. The half-life of a 20mM aqueous solution of PMSF is ~35 minutes at pH 8.0. This short half-life means that aqueous solutions of PMSF can be safely discarded after they have been rendered alkaline (pH >8.6) and stored for several hours at room temperature.
Product Specifications
Catalog ID | P-470 |
---|---|
CAS # | 329-98-6 |
MW | 174.19 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store desiccated at 4°C. Protect from light. |
UN Number | 2923 |
Item Class | 8 (6.1) |
Group Number | II |
Trypsin Inhibitor, Soybean, Purified >10,000 U

Catalog ID | Size | Pricing | |
---|---|---|---|
T-166-100 | 100 mg | $ 108.00 | |
T-166-500 | 500 mg | $ 388.00 | |
T-166-1 | 1 g | $ 692.00 |
Description
Trypsin inhibitor is a serine protease inhibitor from soybeans that is specific for trypsin and trypsin-like proteases. Trypsin inhibitor from soybeans is a monomeric protein containing 181 amino acid residues in a single polypeptide chain cross-linked by two disulfide bridges. Soybean trypsin inhibitor forms a 1:1 complex with the active protease site. Inhibition may be reversed and is pH dependent. The optimal pH for trypsin binding is 8.0.
Product Specifications
Catalog ID | T-166 |
---|---|
CAS # | 9035-81-8 |
Grade | HIGH PURITY GRADE |
Storage/Handling | Store desiccated at -20°C. |