AEBSF is an irreversible serine protease inhibitor. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin, kallikrein and thrombin. It inhibits by acylation of the active site of the enzyme. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25mM. Aqueous solutions are stable between pH 5-6; limited stability above pH 7.5.
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MOLECULAR BIOLOGY GRADE
Formula: C8H10FNO2S · HCl
MW: 239.69 g/mol
Storage and Handling: Store at -20°C. Protect from light.
PubChem Chemical ID: 186136
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store at -20°C. Protect from light.|
Mammalian Protease Inhibitor Cocktail Preparation Protocol
Protease inhibitor cocktail recipe to use during mammalian cell lysis
AEBSF General Information
AEBSF solubility, storage and reactions rates with various serine proteases.
Sample COA - A-540
Sample Certificate of Analysis - AEBSF
COA for example only. Actual tests may differ.