M_Products

Interleukin 4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL4, IL5, IL6, IL10, and IL13, which favor a humoral immune response. Another dominant function of IL4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL4 production by Th2 cells has been associated with elevated IgE production and allergy.

Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.

The IL5 gene is located on chromosome 11 in mice, in close proximity to the genes encoding IL3, IL4, and granulocyte-macrophage colony-stimulating factor (CSF2), which are often co-expressed in TH2 cells. Through binding to the IL5 receptor, IL5 stimulates B cell growth and increases immunoglobulin secretion and is also a key mediator in eosinophil activation. Interleukin 5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. Murine IL5 is a TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL3 and CSF2), this glycoprotein is a homodimer in its active form.

Interleukin-6 (IL6) is a multifunctional protein that plays an important role in host defense, acute phase reactions, immune responses and hematopoiesis. IL6 is expressed by a variety of normal and transformed cells including T-cells, B-cells, monocytes/macrophages, fibroblasts, hepatocytes, keratinocytes, astrocytes, vascular endothelial cells and various tumor cells. The production of IL6 is upregulated by numerous signals, including mitogenic or antigenic stimulation, LPS, calcium ionophore, IL1, IL2, IFN, TNF, PDGF, and viruses. IL6 expression in monocytes is inhibited by IL4 and IL13.

Sterile Filtered White Lyophilized Powder. Purified and tested for use in cell culture.

Interleukin-7 (IL7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. IL7 binds to the IL7 receptor, a heterodimer consisting of IL7 receptor alpha and IL2 receptor gamma chain. IL7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells. It also stimulates the proliferation of B-cells, T-cells and NK cells. Murine IL7 has approximately 61% and 88% amino acid sequence identity with human and rat IL7, and both proteins exhibit cross-species activity. IL7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection.

C-kit ligand, the recently identified ligand for the kit tyrosine kinase receptor, is mapped to the mouse S1 locus. This pleiotropic cytokine, alternately known as stem cell factor (SCF), mast cell growth factor (MGF) and steel-factor (SLF), plays essential roles in gametogenesis, melanogenesis and early stages of hematopoiesis. In vitro and in vivo, KITLG can stimulate the proliferation of mature, as well as the proliferation and maturation of immature, mast cells. KITLG acts in a synergistic manner on purified primitive human and mouse hematopoietic precursors, with various growth factors (such as IL1, IL3, IL6, IL7, and Epo), to induce myeloid, erythroid and lymphoid lineage colony formation. The finding that KITLG is also expressed in the nervous system suggests a possible role for KITLG in the development of the nervous system.

Sterile Filtered White lyophilized powder. Purified and tested for use in cell culture.

L-Malic acid, disodium salt is a dicarboxylic acid used to differentiate microorganisms based on their varying metabolic properties. It is a source of CO₂ in the Calvin cycle and an intermediate of the citric acid cycle.

Methionine functions as a reagent and with metals in non-biological applications, L-methionine in particular has been used with metals to form photosensitizers and ligands for transition metal catalyzed bond forming reactions. [11C]L-Methionine has also been used in Positron Emission Tomography of brain tumors for diagnostic purposes.

Methionine is an alpha amino acid used in protein synthesis. In human beings methionine is a vital amino acid not synthesized in the human body and must therefore be ingested. Methionine in the human body is used to improve skin elasticity and tone, tissue growth and repair, and keratin systems such as hair and nails. As a detoxifying agent methionine chelates heavy metals, while the sulfur in it protects cells. L-Methionine is an enantiomerically active amino acid used in the body.

Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF binds to the specific LIFR-α, which forms a heterodimer containing a GP130 signal transducing subunit.LIF is normally expressed in the trophectoderm of the developing embryo and has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. While LIF is not required for human embryonic stem cell cultures, it is commonly used for mouse embryonic stem cell cultures.

Sterile Filtered White lyophilized powder

Macerozyme R-10 is a macerating enzyme from the Rhizopus sp. which is perfectly suited for the isolation of plant cells. Macerozyme R-10 is often used in combination with Cellulase "Onozuka R-10" in the isolation of plant protoplasts.

Magenta-Gal is used as an alternative to X-Gal, and produces a red color. Magenta-Gal is hydrolyzed by β-galactosidase to produce a red precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce red colonies when grown on plates containing Magenta-Gal.

Magenta-Gal is commonly used in conjunction with IPTG (GoldBIo #I2481) for red/white screening.

Magenta-phosphate p toluidine salt; 5-Bromo-6-chloro-3-indoxyl phosphate, p-toluidine salt

Magnesium sulfate, heptahydrate is a magnesium ion source. Magnesium plays an important role in cell muscle physiology, cardiovascular and muscular activity and nucleic acid structure. Magnesium also functions as a cofactor for restriction enzymes like EcoR I and EcoR V, as well as deoxyribonuclease (DNase).

Magnesium sulfate, heptahydrate can also be used in the protocol for the isolation of chromosomes or nucleic acids.

Mammalian Cell Lysis Buffer has been developed for extraction of total soluble proteins from mammalian cultured cells. The Mammalian Cell Lysis Buffer is based on organic buffering agents, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Mammalian Cell Lysis Buffer. Mammalian Cell Lysis Buffer reagent has been tested for use with a wide variety of mammalian cells. Mammalian Cell Lysis Buffer can be used for both suspension as well as adherent cells. The proprietary combination of this reagent provides a simple and versatile method for the extraction of proteins from mammalian cells.

MANF is a secreted neurotrophic factor that is expressed in brain, neuronal and certain non-neuronal tissues. It has been shown to promote survival, growth and function of dopamine specific neurons. MANF and its structural homolog CDNF each contain an N-terminal saposin-like lipid binding domain and a carboxyl-terminal domain, which is not homologous to previously characterized protein structures. MANF has been identified as a soluble ER stress protein induced by the unfolded protein response. MANF and CDNF can prevent 6-OHDA induced degeneration of dopaminergic neurons by triggering survival pathways, as shown in a rat experimental model of Parkinson disease.

Marbofloxacin is a third generation fluoroquinolone antibiotic used to target Pseudomonades and Staphylococci species. It also targets a variety of other gram-positive and gram-negative bacteria. It is freely soluble in aqueous solution.

Midkine (MDK) is a heparin-binding growth factor that is expressed in various cell types during embryo development and in a variety of carcinomas. It is induced by retinoic acid, as well as during oncogenesis, inflammation, and tissue repair. MDK is pleiotropic and promotes angiogenesis, neurogenesis, chondrogenesis, cell growth, cell migration, and tumorigenesis. It also inhibits apoptosis in some cells. MDK acts through several different receptors including PTP, ALK, PTP-zeta, and LRP. The amino acid sequence of human MDK is about 50% homologous to human pleiotrophin (PTN).

Midkine (MDK) is a heparin-binding growth factor that is expressed in various cell types during embryo development and in a variety of carcinomas. It is induced by retinoic acid, as well as during oncogenesis, inflammation, and tissue repair. MDK is pleiotropic and promotes angiogenesis, neurogenesis, chondrogenesis, cell growth, cell migration, and tumorigenesis. It also inhibits apoptosis in some cells. MDK acts through several different receptors including PTP, ALK, PTP-zeta, and LRP. MDK shows significant homology to pleiotrophin (PTN).

Midkine (MDK) is a heparin-binding growth factor that is expressed in various cell types during embryo development and in a variety of carcinomas. It is induced by retinoic acid, as well as during oncogenesis, inflammation, and tissue repair. MDK is pleiotropic and promotes angiogenesis, neurogenesis, chondrogenesis, cell growth, cell migration, and tumorigenesis. It also inhibits apoptosis in some cells. MDK acts through several different receptors including PTP, ALK, PTP-zeta, and LRP. MDK shows significant homology to pleiotrophin (PTN). The amino acid sequence of Rat MDK is 99% homologous to human MDK.

Meropenem is a carbapenem β-lactam antibiotic. It targets gram-negative and gram-positive bacteria and displays high resistance to β-lactamase and kidney dehydropeptidase I activity. It is especially effective against bacteria responsible for causing meningitis and exhibits low toxicity when compared to similar antibiotics.