L_Products

Lincomycin HCl is a lincosamide antibiotic targeting gram positive and anaerobic bacteria as well as protozoans. It is commonly used to combat Staphylococcus and Streptococcus bacteria. Lincomycin HCl is freely soluble in aqueous solution.

Low Melting (LM) Agarose is a low melting temperature agarose with the highest resolving capacity for large DNA fragments, including PCR products.

Low melting temperature allows for the recovery of undamaged nucleic acids below denaturation temperature. Low gelling temperature ensures In-Gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps.

Low Melting Agarose is ideal for digestion by agarose enzymes, which makes it very easy to recover large DNA fragments suitable for cloning or enzymatic processing.

Horseradish peroxidase is a common marker enzyme for antibodies, and can catalyze the chemiluminescent oxidation of luminol in the presence of hydrogen peroxide. This transformation is used in ultra-sensitive ELISA assays. Luminescence can be detected at very low levels using extremely sensitive scintillation counters.

Luminol is also used for the detection of blood stains in criminal investigation. For this, luminol is mixed with a liquid containing hydrogen peroxide. If the mixture comes in contact with hemoglobin, the iron in the hemoglobin accelerates chemiluminescent oxidation of luminol.

Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.

Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.

Mammalian Cell Lysis Buffer has been developed for extraction of total soluble proteins from mammalian cultured cells. The Mammalian Cell Lysis Buffer is based on organic buffering agents, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Mammalian Cell Lysis Buffer. Mammalian Cell Lysis Buffer reagent has been tested for use with a wide variety of mammalian cells. Mammalian Cell Lysis Buffer can be used for both suspension as well as adherent cells. The proprietary combination of this reagent provides a simple and versatile method for the extraction of proteins from mammalian cells.

N-Acetyl-L-cysteine, or N-acetylcysteine (abbreviated NAC) is an acid derivative of L-cysteine capable of reducing free radicals and complexing palladium, helping it dissolve in water. Biofilms of certain pathogens such as Staphylococcus aureus and Enterobacter cloacae are broken down by NAC, giving it bactericidal properties.

GoldBIo Orange 6x DNA Loading Dye is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains one dye (Orange G) for tracking the DNA migration.

The Orange G migrates at approximately 50 bp on a standard 1% TAE agarose gel (30 bp on 2% TAE agarose gel). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition

• 0.15% Orange G
• 10mM Tris-HCl (pH 8.0)
• 60% glycerol
• 60mM EDTA

Protein L Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification and immunoprecipitation. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein L. Magnetic agarose beads provide a convenient and quick method for purifications of IgG, IgM, IgA, and IgD containing kappa light chains from various species without interfering with the antigen binding site.

Protein L Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

rac-1-Lauroylglycerol is a monoglyceride. It’s typically used as a surfactant and emulsifier in food applications, such as the extension of shelf life. In pharmacology it possesses antimicrobial and antiviral properties, believed to work by binding with the surface of viruses and bacteria and disrupting their outer envelopes. It does not seem to generate resistance in bacteria like some other antibiotics.

GoldBio's SuperLadder (50 bp-25 kb) is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder is composed of 26 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100 and 50 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA.

This product contains four enhanced bands (3k, 1.2k, 500 and 200 bp) for easier reference. In addition, three tracking dyes are present (Xylene cyanol FF, Bromophenol blue and Orange G) which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA respectively during electrophoresis on a 1% TAE agarose gel which helps in real time monitoring.

Tissue Cell Lysis Buffer has been developed for extraction of total soluble protein from fresh or frozen animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue Cell Lysis Buffer. Tissue Cell Lysis Buffer reagent has been tested for use with a wide variety of animal tissues.

GoldBio Tri-Color 6x DNA Loading Dye is a pre-mixed buffer for tracking DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains three dyes (Xylene cyanol FF, Bromophenol blue and Orange G) for tracking the DNA migration.

The Xylene cyanol FF, Bromophenol blue and Orange G migrate at approximately 4,000 bp, 500 bp and 50 bp on 1% TAE agarose gel respectively (800 bp, 150 bp and 30 bp on a standard 2% TAE agarose gel respectively). Glycerol is used to keep the DNA at the bottom of the well. The presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition

• 0.03% Xylene cyanol FF
• 0.03% Bromophenol blue
• 0.15% Orange G
• 10mM Tris-HCl (pH 8.0)
• 60% glycerol
• 60mM EDTA

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 19 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-10,000 base pairs. The 500, 1.5K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

The Yeast Lysis Buffer Kit is useful for extraction of soluble proteins from yeast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

This kit is provided with an optional protocol to make spheroplast and remove lytic enzyme Zymolyase®, prior to lysis and extraction of yeast proteins. Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer.

The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works with both gram-positive and gram-negative bacteria. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.

This kit is suitable for processing 100 x 50 µl yeast cell pellet preparations. Yeast Lysis Buffer is also available separately for further downstream processing.

The Yeast Spheroplast Lysis Buffer is useful for extraction of soluble proteins from spheroplast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer. The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works well with both gram-positive and gram-negative bacteria.

The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.