SuperLadder, (50 bp-25 kb) DNA Ladder

GoldBio's SuperLadder (50 bp-25 kb) is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder is composed of 26 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100 and 50 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA.

This product contains four enhanced bands (3k, 1.2k, 500 and 200 bp) for easier reference. In addition, three tracking dyes are present (Xylene cyanol FF, Bromophenol blue and Orange G) which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA respectively during electrophoresis on a 1% TAE agarose gel which helps in real time monitoring.

Benefits

• Sharp bands
• Quick reference— enhanced bands
• Ready-to-use— premixed with loading dye for direct loading
• Stable— room temperature storage up to 6 months

Source

Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10mM Tris-HCl (pH 8.0) and 10mM EDTA.

Range

50 - 25,000 bp

Concentration

88.7 µg / 500 µL

Recommended loading volume

5 µL / well

Storage

Store at room temperature for up to 6 months
For long term storage, store at 4°C for up to 12 months or at -20°C for up to 36 months.