YEM Media

Product Description

Yeast Extract Mannitol (YEM) media is often used for the growth of Agrobacterium as well as Rhizobium bacterial strains. Mannitol is used as a sugar substitute to provide a carbon source while the yeast extract provides the nitrogen for bacterial growth.

YEM media is a complex media formulation that provides optimal and stress free growing conditions for cultures of both Agrobacterium and Rhizobia.

GoldBio's YEM media is sterile and is a Ready-To-Use liquid solution based on the original formulation by A. Untergasser.

GoldBio's lab has found that specifically, LBA4404 Agrobacterium competent cells are most sensitive to culture conditions and perform best using YEM media.

Storage/Handling: Store at 4°C.
Do NOT autoclave.

YEM Media Formulation: Per 0.5 Liter


2.5 g

Yeast extract

0.25 g

MgSO4 x 7 H2O

0.1 g


0.05 g


0.25 g

Sodium Gluconate

2.5 g


0.083 g


Untergasser A. “Media - YEM (YEast extract - Manitol)” Untergasser's Lab. Summer 2008

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$12.99 Ground shipping (In continental US only.)

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