Enzyme Substrates

Gold Biotechnology offers an extensive amount of enzyme substrates that are ideal for your research needs. These substrates include chromogenic and fluorogenic substrates such as firefly luciferin, MUG, X- α-Gal, X-Gluc, as well as many others. Our superior substrates are ideal for a number of biological applications and are available for a much more affordable price so that your research dollars are focused on the things that matter.
Showing 121-136 of 136 items.

Resorufin-β-D- glucuronic acid sodium salt

Catalog ID Size Pricing
R-100-2 2 mg $ 427.00
R-100-5 5 mg $ 851.00
R-100-10 10 mg $ 1,362.00
R-100-25 25 mg $ 2,696.00

Description

Resorufin-β-D- glucuronic acid is a fluorogenic substrate for β-glucuronidase, yielding resorufin upon cleavage.

Product Specifications

Catalog ID R-100
CAS # 125440-91-7
MW 411.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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R-100-2 2-3 Weeks 2 mg $ 427.00
R-100-5 In stock 5 mg $ 851.00
R-100-10 2-3 Weeks 10 mg $ 1,362.00
R-100-25 2-3 Weeks 25 mg $ 2,696.00

S-Acetylthiocholine iodide

Catalog ID Size Pricing
A-162-25 25 g $ 219.00
A-162-50 50 g $ 364.00
A-162-100 100 g $ 582.00

Description

No description available

Product Specifications

Catalog ID A-162
CAS # 1866-15-5
Grade MOLECULAR BIOLOGY GRADE
MW 289.18 g/mol
Storage/Handling Store desiccated at 4°C. Protect from light.
UN Number 2811
Item Class 6.1
Group Number III
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A-162-25 2-3 Weeks 25 g $ 219.00
A-162-50 2-3 Weeks 50 g $ 364.00
A-162-100 2-3 Weeks 100 g $ 582.00

S-Butyrylthiocholine iodide

Catalog ID Size Pricing
B-820-10 10 g $ 131.00
B-820-25 25 g $ 276.00
B-820-50 50 g $ 524.00
B-820-100 100 g $ 814.00

Description

No description available

Product Specifications

Catalog ID B-820
CAS # 1866-16-6
MW 317.23 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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B-820-10 2-3 Weeks 10 g $ 131.00
B-820-25 2-3 Weeks 25 g $ 276.00
B-820-50 2-3 Weeks 50 g $ 524.00
B-820-100 2-3 Weeks 100 g $ 814.00

Salmon Gal (6-Chloro-3-indoxyl-β-D-galactopyranoside)

Catalog ID Size Pricing
C-255-500 500 mg $ 69.00
C-255-1 1 g $ 117.00
C-255-2.5 2.5 g $ 257.00
C-255-5 5 g $ 385.00
C-255-10 10 g $ 641.00

Description

Salmon Gal (6-Chloro-3-indolyl-β-D-galactopyranoside) is used with IPTG as an alternative chromogenic substrate capable of detecting LacZ gene encoded ß-galactosidase activity for colony screening in colometric assays.

Unlike colony blue/white screening through X-Gal, nonrecombination results in a salmon color after salmon gal is cleaved by ß-galactosidase through hydrolysis. This contrast may be useful in comparative or secondary screenings.

Incorporating nitroblue tetrazolium salt (NBT) into the salmon gal reactions provides faster results with a clear, purple precipitate than in unaccompanied uses of salmon gal.

Product Specifications

Catalog ID C-255
CAS # 138182-21-5
MW 329.73 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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C-255-500 2-3 Weeks 500 mg $ 69.00
C-255-1 In stock 1 g $ 117.00
C-255-2.5 2-3 Weeks 2.5 g $ 257.00
C-255-5 2-3 Weeks 5 g $ 385.00
C-255-10 2-3 Weeks 10 g $ 641.00

TMB dihydrochloride

Catalog ID Size Pricing
TMBHCL2 2 g $ 110.00
TMBHCL10 10 g $ 482.00
TMBHCL25 25 g $ 926.00
TMBHCL50 50 g $ 1,421.00

Description

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.

Product Specifications

Catalog ID TMBHCL
CAS # 207738-08-7
MW 313.27 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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TMBHCL2 In stock 2 g $ 110.00
TMBHCL10 In stock 10 g $ 482.00
TMBHCL25 In stock 25 g $ 926.00
TMBHCL50 2-3 Weeks 50 g $ 1,421.00

TMB, free base

Catalog ID Size Pricing
TMBF10 10 g $ 80.00
TMBF25 25 g $ 160.00
TMBF50 50 g $ 289.00
TMBF100 100 g $ 513.00
TMBF250 250 g $ 1,200.00

Description

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.

Product Specifications

Catalog ID TMBF
CAS # 54827-17-7
MW 240.35 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at 4°C. Protect from light.
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TMBF10 2-3 Weeks 10 g $ 80.00
TMBF25 2-3 Weeks 25 g $ 160.00
TMBF50 2-3 Weeks 50 g $ 289.00
TMBF100 2-3 Weeks 100 g $ 513.00
TMBF250 2-3 Weeks 250 g $ 1,200.00

Tyramine, free base

Catalog ID Size Pricing
T-450-500 500 g $ 250.00
T-450-1 1 kg $ 388.00

Description

No description available

Product Specifications

Catalog ID T-450
CAS # 51-67-2
MW 137.18 g/mol
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T-450-500 2-3 Weeks 500 g $ 250.00
T-450-1 2-3 Weeks 1 kg $ 388.00

X-Gal

Catalog ID Size Pricing
X4281C 1 g $ 59.00
X4281C10 10 g $ 289.00

Description

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.

X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Bacteria cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.

Many other applications also use X-Gal as a substrate to detect β-galactosidase activity. These include β-galactosidase -antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction and the detection of micrometastasis formation during tumor progession.

Product Specifications

Catalog ID X4281
Name(s) X-Gal;
5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside
CAS # 7240-90-6
Grade MOLECULAR BIOLOGY GRADE
Formula C14H15BrClNO6
MW 408.63 g/mol
Biological Activity Blue color with Lambda, pUC, and M13
PubChem Chemical ID 619018
Storage/Handling Store at -20°C. Protect from light.
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X4281C In stock 1 g $ 59.00
X4281C10 In stock 10 g $ 289.00

X-gluc (CHX salt)

Catalog ID Size Pricing
G1281C 100 mg $ 59.00
G1281C1 1 g $ 205.00
G1281C2 2 x 1 g $ 395.00
G1281C3 3 x 1 g $ 555.00
G1281C4 4 x 1 g $ 665.00
G1281C5 5 g $ 765.00

Description

X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate. X-Gluc is often used in GUS staining of plant cells and tissue and in infections caused by E. coli.

Product Specifications

Catalog ID G1281
CAS # 114162-64-0
MW 521.79 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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G1281C In stock 100 mg $ 59.00
G1281C1 In stock 1 g $ 205.00
G1281C2 In stock 2 x 1 g $ 395.00
G1281C3 In stock 3 x 1 g $ 555.00
G1281C4 In stock 4 x 1 g $ 665.00
G1281C5 In stock 5 g $ 765.00

X-pal

Catalog ID Size Pricing
B-470-100 100 mg $ 113.00
B-470-250 250 mg $ 219.00
B-470-500 500 mg $ 388.00
B-470-1 1 g $ 713.00

Description

X-pal is an off white to light brownish powder that is used as a chromogenic substrate for carboxylesterase. X-pal yields a blue precipitate when cleaved.

Product Specifications

Catalog ID B-470
CAS # 341972-98-3
MW 484.91 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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B-470-100 2-3 Weeks 100 mg $ 113.00
B-470-250 2-3 Weeks 250 mg $ 219.00
B-470-500 2-3 Weeks 500 mg $ 388.00
B-470-1 2-3 Weeks 1 g $ 713.00

X-sulfate K

Catalog ID Size Pricing
B-520-1 1 g $ 497.00
B-520-2.5 2.5 g $ 1,145.00
B-520-5 5 g $ 2,069.00

Description

X-Sulfate K is a slightly off-white to faint-yellowish powder that is used as a chromogenic substrate for aryl-sulfatase. X-Sulfate K yields a blue precipitate when cleaved.

Product Specifications

Catalog ID B-520
CAS # 6578-07-0
MW 364.65 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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Packs & Pricing
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B-520-1 2-3 Weeks 1 g $ 497.00
B-520-2.5 2-3 Weeks 2.5 g $ 1,145.00
B-520-5 2-3 Weeks 5 g $ 2,069.00

X-α-Gal

Catalog ID Size Pricing
XA250 250 mg $ 115.00
XA500 500 mg $ 195.00
XA1 1 g $ 295.00
XA5 5 g $ 1,175.00
XA10 10 g $ 1,995.00

Description

X-α-Gal is a chromogenic substrate for α-galactosidase, yielding a blue precipitate. X-α-Gal is used for detecting two-hybrid interactions directly on the culture media. This substrate eliminates the need for β-galactosidase liquid and filter-lift assays in two-hybrid analysis.
Used for species differentiation within the family Enterobacteriaceae and differentiation of Bifidobacteria species from Lactobacilli species.

Product Specifications

Catalog ID XA
CAS # 107021-38-5
MW 408.64 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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Packs & Pricing
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XA250 In stock 250 mg $ 115.00
XA500 In stock 500 mg $ 195.00
XA1 In stock 1 g $ 295.00
XA5 In stock 5 g $ 1,175.00
XA10 In stock 10 g $ 1,995.00

X-α-L-fucopyranoside

Catalog ID Size Pricing
B-360-100 100 mg $ 255.00
B-360-250 250 mg $ 472.00
B-360-500 500 mg $ 800.00

Description

α-L-Fucosidases are carbohydrate-processing enzymes belonging to the exoglycosidase family of glycoside hydrolases. These enzymes break down glycosidic bonds of sugars at the terminal ends. Fucose –containing glycolipids and glycoproteins can accumulate in different tissues when α-L-Fucosidase is inactive.

X-α-L-fucopyranoside is used as a chromogenic substrate for α-L-Fucosidase, producing a blue precipitate.

Product Specifications

Catalog ID B-360
CAS # 171869-92-4
MW 392.64 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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Packs & Pricing
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B-360-100 2-3 Weeks 100 mg $ 255.00
B-360-250 2-3 Weeks 250 mg $ 472.00
B-360-500 In stock 500 mg $ 800.00

X-β-D-fucopyranoside

Catalog ID Size Pricing
B-370-50 50 mg $ 421.00
B-370-100 100 mg $ 527.00
B-370-250 250 mg $ 822.00
B-370-500 500 mg $ 1,028.00
B-370-1 1 g $ 1,285.00

Description

No description available

Product Specifications

Catalog ID B-370
CAS # 17016-46-5
MW 392.64 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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Packs & Pricing
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B-370-50 2-3 Weeks 50 mg $ 421.00
B-370-100 2-3 Weeks 100 mg $ 527.00
B-370-250 2-3 Weeks 250 mg $ 822.00
B-370-500 2-3 Weeks 500 mg $ 1,028.00
B-370-1 2-3 Weeks 1 g $ 1,285.00

Y-β-D-GLC

Catalog ID Size Pricing
I-470-250MG 250 mg $ 93.00
I-470-5 5 g $ 867.00

Description

Y-β-D-GLC is a chromogenic substrate for β-D-glucosidase, yielding a blue precipitate upon cleavage.

Product Specifications

Catalog ID I-470
CAS # 487-60-5
MW 295.29 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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Packs & Pricing
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I-470-250MG 2-3 Weeks 250 mg $ 93.00
I-470-5 2-3 Weeks 5 g $ 867.00

α-D-Fucose

Catalog ID Size Pricing
F-850-1 1 g $ 77.00
F-850-2 2 g $ 124.00
F-850-5 5 g $ 242.00
F-850-10 10 g $ 437.00
F-850-25 25 g $ 968.00

Description

α-D-Fucose is a six-carbon deoxy monosaccharide. It is equivalent to 6-deoxy-D-galactose. D-fucose acts as a non-metabolizable analogue of L-arabinose and inhibits induction of the L-arabinose operon in some bacteria. Some resistant bacteria possess a mutant araC gene where D-fucose acts as a gratuitous inducer of the L-arabinose operon. D-fucose induces β-galactosidase to some extent in Lactobacillus plantarum.

α-D-fucose inhibits the eukaryotic enzyme Lysine-specific demethylase 1 (LSD1A), thereby causing gene repression. α-D-fucose is being explored in treatments for neurodegenerative diseases because of its effect on gene repression. Seen upregulated in many cancers, LSD1A overexpression pushes the cell cycle of otherwise non-transformed cells so α-D-fucose is being explored in cancers treatments as well.

Product Specifications

Catalog ID F-850
CAS # 3615-37-0
MW 164.16 g/mol
Storage/Handling Store at 4°C.
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Packs & Pricing
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F-850-1 2-3 Weeks 1 g $ 77.00
F-850-2 2-3 Weeks 2 g $ 124.00
F-850-5 2-3 Weeks 5 g $ 242.00
F-850-10 2-3 Weeks 10 g $ 437.00
F-850-25 2-3 Weeks 25 g $ 968.00

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