X-gluc (CHX salt)
X-Gluc is a chromogenic substrate for beta-glucuronidase (GUS) which is encoded by the gusA (uid A) gene, a widely used reporter gene. Glucuronidase cleaves X-Gluc to produce colorless glucuronic acid and an intense blue precipitate. X-Gluc is often used in GUS staining of plant cells and tissue and in infections caused by E. coli.
(X-glu, X-glcA, BCIG)
5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt
MOLECULAR BIOLOGY GRADE
Formula: C14H13BrClNO7 • C6H13N
MW: 521.79 g/mol
Storage/Handling: Store desiccated at -20°C. Protect from light.
Soluble in DMF.
Purity: greater than 99% (HPLC)
Thin Layer Chromatogram: single spot
Biological Activity: indigo-blue assay
Elemental Analysis: Agrees with theoretical values
PubChem Chemical ID: 16760327
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store desiccated at -20°C. Protect from light.|
Histochemical Staining of GUS Reporter Activity
Pollen Staining for GUS Activity - adapted from a protocol by Dr. Daphne Preuss.
Gus Gene Assay in Transformed Plant Tissue
Procedures for GUS gene expression, fluorimetric and fluorigenic assays by Dr. Paul Bottino, Plant Molecular Genetics (retired), University of Maryland.
Sample COA - G1281
Sample Certificate of Analysis - X-gluc
COA for example only. Actual tests may differ.