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GoldBio BSA is a highly purified, lyophilized albumin, suitable for Westerns, enzyme systems, protein standards, hybridizations and as a protease-sensitive immunoassays. It is also perfectly suitable as a nutrient in cell culture media, with low interference and backgrounds and is ideal for most standard molecular biology experiments. Our BSA is protease and certified TSE free, manufactured in the United States in a closed loop system from USDA-inspected, healthy animals located in the US.

GoldBio’s C58C1 Chemically Competent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 10⁵ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s C58C1 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. C58C1 has the chromosomal backbone of C58 but it has been cured of the Ti plasmid pTiC58. Our C58C1 strain harbors streptomycin and rifampicin resistance genes. C58C1 Competent cells are optimized for genetic transformation of plants such as Arabidopsis but can be used in many other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: C58C1
Transformation efficiency: ≥1 x 10⁷ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

D-Arabitol is the reduced alcohol form of D-arabinose. It is produced by some fungus from enzymatic dephosphorylation and reduction of D-ribulose-5-phosphate. D-arabitol is metabolized by a variety of different bacteria utilizing an arabitol dehydrogenase enzyme. The arabitol dehydrogenase gene has been introduced into plants to serve as a positive selection marker for transgenic cells.

Arginine is an essential amino acid found in food and plant sources, and many biological systems. D-Arginine specifically can inhibit arginine decarboxylase enzymes and also functions as a metabolite.

DL-Arginine is an alpha amino-acid and can be found as a metabolite and as a reagent for biological molecules.

GoldBio’s EHA105 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥5 x 10³ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

EHA105 (pSoup) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥4 x 10⁸ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s EHA105 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.

The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.

GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥2 x 10³ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

EHA105 (pSoup-P19) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥3 x 10⁸ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥5 x 10⁴ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

GoldBio’s EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥1.6 x 10⁸ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

2-Acetamido-2-deoxy-3-O-(a-D-galactopyranosyl)-D-galactopyranose is an amino disaccharide metabolite used in carbohydrate chemistry. It is a deoxy sugar derivate, rendered from a deoxyribose molecule. This sugar qualifies as a member of the acetamide group due to the organic compound, acetamide, contained within it. It is also a glycosyl compound, a sugar group which has a glycosidic binding to another group through its anomeric carbon. 2-Acetamido-2-deoxy-3-O-(a-D-galactopyranosyl)-D-galactopyranose can be used as a chiral reagent.

Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (40-60 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.

The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Glyoxal beads allow a covalent binding of agarose to lysine amino groups (similar orientation to CNBr). Glyoxal agarose beads are ready to use and require no activation. The resin has a high amount of aldehydes groups (15-25 µmoles Glyoxal / ml gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand.

The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.

Glyoxal HTC 6% HIGH Density Agarose beads allow covalent binding of agarose to lysine amino groups (similar orientation to CNBr).

This product is adequate to work in large scale process (High Pressure). The resin has a high amount of aldehyde groups (40-60 µmoles Glyoxal / ml gel) providing multiple binding points and high immobilized ligand stability. The resin has been manufactured using 6% HTC agarose beads, and the pore size is adequate to work with enzymes, proteins & small ligands. The stability at high flow is adequate for large scale process.

GoldBio’s GV3101 Agrobacterium chemically competent cells are a powerful tool for high efficiency transformation of plants. The GV3101 strain is optimized for cDNA and gDNA library construction, and possesses resistance to rifampicin, gentamicin, and tetracycline. The T-DNA region of the GV3101 Ti plasmid has been deleted, but restoration of the binary vector T-region can fully enable T-DNA binary system function.

The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.

Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101 (pSoup)
Transformation efficiency: ≥1.5 x 10³ cfu/µg pCAMBIA1391z DNA
Blue/white screening: No