Troubleshooting PCR Part Two: What to Do When You Are Not Getting Bands
PCR (Polymerase Chain Reaction), which is so common to the lab, can be very frustrating. We unders...
GoldBio's Caprylate Stabilized BSA is a highly purified, lyophilized albumin, suitable for Westerns, enzyme systems, protein standards, hybridizations and as a protease-sensitive immunoassays. It is also perfectly suitable as a nutrient in cell culture media, with low interference and backgrounds and is ideal for most standard molecular biology experiments.
Caprylate stabilized BSA has exceptional purity and low interference, utilizing heat-shock fractionation designed to inactivate proteases and other potentially interfering enzymes.
BSA protein is a single polypeptide chain consisting of ~583 amino acid residues and no carbohydrates and is approximately 66 kDa in size. Fraction V refers to the fifth fraction of the original Cohn purification of plasma proteins using cold ethanol precipitation and is still commonly used as a name for bovine serum albumin regardless of purification method.
GoldBio BSA is protease and certified TSE free, manufactured in the United States in a closed loop system from USDA-inspected, healthy animals located in the US.
Protein (on Dried Basis) |
≥98.0% |
Purity (albumin) |
≥98.0% |
Moisture |
≤5.0% |
pH (10% in Water) |
6.5 to 7.5 |
Ash (Residue on Ignition) |
<2.0% |
Protease (Enzymatic Assay) |
≤0.001 Units/mg |
Endotoxin |
≤1 EU/mg |
Heavy Metals (Pb) |
≤10 ppm |
IgG |
None Detected |
Store at 4°C.
Name(s) |
Albumin Bovine Serum (Caprylate Stabilized), Fraction V; BSA, Bovine Albumin |
---|---|
Catalog ID | A-420 |
CAS # | 9048-46-8 |
MW | ~66 kDa |
Grade | BIOTECHNOLOGY GRADE |
Purity | ≥98.0% |
Storage/Handling | Store at 4°C. |
PCR (Polymerase Chain Reaction), which is so common to the lab, can be very frustrating. We unders...
Bovine Serum Albumin (BSA) comes in many forms, which is why people frequently post questions onli...
Bovine Serum Albumin (BSA) is universal in the lab, having use in Western blot, cell tissue cultur...
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