Articles

How Biotinylated Molecules are Purified with Streptavidin Beads

Streptavidin is a bacterial protein that binds to the small molecule biotin with extremely high affi...

Understanding Salting In and Salting Out: Salt as a Protein Purification Tool

Salt is a major component in protein purification buffers, and optimizing this important parameter c...

How to Use Magnetic Agarose Beads to Purify Antibodies: A Step-by-Step Protocol

This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, o...

Regular vs Magnetic Agarose Beads: Key Differences and Best Uses

Have you ever wondered whether you should be using regular agarose beads or magnetic agarose beads f...

The Purpose of Magnetic Agarose Beads

Have you ever seen a cartoon where a huge magnet is used to pull objects into the sky? Usually, the...

Easy Step-by-Step Antibody Purification Protocol with Protein A, Protein G, and Protein L Agarose Beads

In many articles, we’ve conceptually discussed Protein A, G, and L agarose beads and how purifying a...

How to Perform Immunoprecipitation with Protein A, G, or L Agarose Beads

Immunoprecipitation (IP) is a powerful and frequently used technique that utilizes antibodies to iso...

How Much Resin for My Purification When Using Protein A, G or L? Binding Capacity Holds Answers

When affinity purifying a protein, one of the first questions you’ll ask yourself is “how much resin...

Choosing Between Proteins A, G, and L: Key Similarities and Differences

Have you heard of Protein A, Protein G, and Protein L? Maybe you know that you need one of them to p...