Library Preparation Methods for Sequencing
Library preparation is a method where DNA or cDNA is attached to a flow cell (a slide with tiny chan...
GV3101 Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-207-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-207-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-207-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pmp90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥2 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Agrobacterium Electrocompetent Cells from GoldBio
GoldBio’s high efficiency GV3101 Agrobacterium Electrocompetent Cells are ideal for applications such as cDNA or gDNA library construction.
The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pmp90 (pTiC58DT-DNA) with gentamicin resistance.
Quick Specifications
| Transformation Efficiency | ≥1 x 107 cfu/µg pCAMBIA1391z DNA |
| Reagents for One Reaction | * GV3101 Electrocompetent Agrobacterium: 25 µl * DNA (pCAMBIA1391z, 500 pg/µl): 1 µl * Recovery medium: 1 ml |
| Plasmid Information | Ti plasmid pmp90 (pTiC58DT-DNA) |
| Resistance Information | Gentamicin and Rifampicin resistance |
| Plant Information |
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Additional Details
The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant S genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato and soybeans as well as monocots like corn.
Storage/Handling: This product may be shipped on dry ice. GV3101 Agrobacterium Electrocompetent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.
