Protein Gel Staining

Detection of proteins immobilized in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is an essential step in Western blots and protein analysis. GoldBio offers a variety of easy-to-use in-gel dye-based stains that diffuse into the gel allowing you to visualize your protein and accurately perform protein quantification. Our Coomassie Brilliant Blue G-250 and R250 dyes produce a robust signal in SDS-PAGE and blue native (BN-PAGE) and are compatible with downstream applications including protein extraction, sequencing and mass spectrometry. We also carry a safer and faster alternative to Coomassie stains, Blazin’ Blue™ Protein Gel Stain, which allows detection of protein through visible blue staining or by near-infrared fluorescence. Alternatively, you can detect your protein with our highly sensitive Blazin’ Bright™ Luminescent Protein Gel Stains, which can stain as little as 1-10 ng of protein in a single step and without the need for fixation.

Showing 1-5 of 5 items.

Blazin' Blue™ Protein Gel Stain

Catalog ID Size Pricing
P-810-1 1 L $ 106.00

Description

Blazin' Blue™ Protein Gel Stain is a ready-to-use protein gel staining solution that is a safer and more effective replacement for traditional Coomassie stains. Blazin' Blue™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation or washing. Protein bands can be detected through visible blue staining or by near-infrared fluorescence. And since Blazin' Blue™ is aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Blue™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Product Specifications

Catalog ID P-810
Storage/Handling Store at 4°C.
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P-810-1 1 L $ 106.00

Blazin' Bright™ Luminescent Protein Gel Stain

Catalog ID Size Pricing
P-820-1 1 L $ 138.00

Description

Blazin' Bright™ Luminescent Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Blazin' Bright™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation (destaining is optional). The Blazin' Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin' Bright™ has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin' Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Product Specifications

Catalog ID P-820
Storage/Handling Store at room temperature.
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P-820-1 1 L $ 138.00

Blazin' Bright™ Luminescent UV Protein Gel Stain

Catalog ID Size Pricing
P-825-1 1 L $ 187.00

Description

Blazin' Bright™ Luminescent UV Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Gels stained with our Blazin' Bright™ Luminescent UV Protein Gel Stain can be visualized rapidly (5-30 minutes) in a single step without fixation (destaining is optional). The Blazin' Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.

Blazin' Bright™ Luminescent UV has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin' Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.

Product Specifications

Catalog ID P-825
Storage/Handling Store at 4°C.
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P-825-1 1 L $ 187.00

Coomassie Brilliant Blue G-250

Catalog ID Size Pricing
C-460-5 5 g $ 29.00
C-460-25 25 g $ 39.00
C-460-50 50 g $ 75.00

Description

Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family and is used as a common analytical dye in SDS-PAGE and BN-PAGE. CBBG can also be used with the Bradford Method to determine protein concentration.

Product Specifications

Catalog ID C-460
CAS # 6104-58-1
MW 854.02 g/mol
Storage/Handling Store desiccated at room temperature.
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C-460-5 5 g $ 29.00
C-460-25 25 g $ 39.00
C-460-50 50 g $ 75.00

Coomassie Brilliant Blue R-250

Catalog ID Size Pricing
C-461-5 5 g $ 35.00
C-461-25 25 g $ 49.00
C-461-100 100 g $ 85.00

Description

Coomassie Brilliant Blue R-250 (CBBR) is a member of the Coomassie family and is used extensively as an analytical dye in SDS-PAGE.

Product Specifications

Catalog ID C-461
CAS # 6104-59-2
MW 825.97 g/mol
Storage/Handling Store desiccated at room temperature.
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C-461-5 5 g $ 35.00
C-461-25 25 g $ 49.00
C-461-100 100 g $ 85.00

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