Protein Ladders: Prestained Versus Unstained
There are two categories of commercially available protein ladders, unstained protein ladders and pr...
Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family of triphenylmethane dyes and is used as a common analytical dye in SDS-PAGE and BN-PAGE. CBBG can also be used with the Bradford Method to determine protein concentration. G-250 is differentiated from the R-250 Coomassie stain by the addition of two methyl groups and the slightly greenish tint to its blue color. CBBG may exist in one of three forms: a cation (red form) at a pH below 0 with an absorbance peak at 470 nm, an anion (blue form) at a pH above 2 with an absorbance peak at 595 nm, and a neutral, green form at a pH around 1 with an absorbance peak around 650 nm. The blue, anionic form is what binds with amino acid residues, such as arginine or aromatics, to form a stable complex—forming the basis of the Bradford Method.
Recently, it has also been shown that CBBG is also an antagonist to P2X7 (P2X purinoceptor 7: P2RX7). CBBG is soluble in water but methanol and acetic acid are often added for better solubility.
Comassie Brilliant Blue G-250, Ultra Pure
CBBG, Ultra Pure
MW: 854.02 g/mol
Storage/Handling: Store in a dry and well-ventilated place.
PubChem Chemical ID: 61363
|Storage/Handling||Store desiccated at room temperature.|
In Gel Digestion of proteins - including destaining, alkylation, digestion and recovery.
Western blotting and immunodetection procedure for protein characterization and analysis.
Sample Certificate of Analysis - Coomassie Brilliant Blue G-250
COA for example only. Actual tests may differ.