Five Tips To Make Your Research Antibiotics Last Longer
How do you make research antibiotics last longer? This is a common question in the lab, and research...
Cefotaxime is a third generation, broad-spectrum cephalosporin antibiotic effective against various gram-negative and gram-positive bacteria. However, unlike many other cephalosporins, cefotaxime is not effective against Pseudomonas aeruginosa bacteria. Cefotaxime is often used in Agrobacterium tumefaciens-mediated transformations to regulate bacterial growth. It has been shown to exhibit a synergistic effect with Vancomycin in certain conditions.
Cephalosporins are a type of β-lactam antibiotic consisting of a four-membered β-lactam ring bound to a six-membered dihydrothiazine ring. This two-ring system causes distortion of the β-lactam amide bond, resulting in decreased resonance stabilization and increased reactivity. β-lactams inhibit the formation of peptidoglycan cross-links within bacterial cell walls by targeting penicillin-binding proteins or PBPs. Consequently, the bacterial cell wall becomes weak and cytolysis occurs. Cephalosporins are less susceptible to β-lactamases than the penicillin β-lactam antibiotics.
The Cefotaxime (Sodium) EZ Pak™ is the fastest and easiest way to make a set amount of sterile cefotaxime (sodium) solution. The kit includes pre-weighed cefotaxime (sodium) powder, a sterile filter and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio cefotaxime (sodium) and the sterile solution is ready for tissue culture, bacterial media or any number of uses.
The 25 ml, 50 ml, and 100 ml requires a vacuum manifold for easy vacuum filtering, while the 10 ml pack sizes come with a syringe filter.
Agrobacterium tumefaciens-mediated transformation of Colletotrichum graminicola - method by Flowers and Vaillancourt (2005)
A brief explanation of the disk diffusion assay method used to test Gold Bio antibiotics
Examine the Effect of Carbenicilln, Cefotaxime and Vancomycin on Plant Morphogenesis by Teixeira da Silva, JA and S. Fukai (2001)
Sample Certificate of Analysis - Cefotaxime
COA for example only. Actual tests may differ.