E_Products
GB5-alpha™ Electrocompetent E. coli Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-203-5x50 | 5 x 50 μL | $ 119.00 | |
CC-203-5x100 | 5 x 100 μL | $ 185.00 | |
CC-203-10x50 | 10 x 50 μL | $ 215.00 | |
CC-203-15x50 | 15 x 50 μL | $ 295.00 | |
CC-203-10x100 | 10 x 100 μL | $ 315.00 | |
CC-203-15x100 | 15 x 100 μL | $ 399.00 |
Description
GoldBio’s GB5-alpha™ Electrocompetent E. coli are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. Plasmid quality and yields are both enhanced in our GB5-alpha™ cells due to mutations in both endA1 and recA1.
Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes
Gentamicin Sulfate EZ Pak™ for 50 mg/mL Solution
Catalog ID | Size | Pricing | |
---|---|---|---|
G-400-EZ10 | 10 mL | $ 59.00 | |
G-400-EZ25 | 25 mL | $ 85.00 | |
G-400-EZ50 | 50 mL | $ 115.00 | |
G-400-EZ100 | 100 mL | $ 145.00 |
Description
The Gentamicin EZ Pak™ is the fastest and easiest way to make a set amount of sterile gentamicin solution. The kit includes pre-weighed gentamicin powder, a sterile filter and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio gentamicin and the sterile solution is ready for tissue culture, bacterial media, or any number of uses.
Product Specifications
Catalog ID | G-400-EZ |
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CAS # | 1405-41-0 |
MW | 575.67 g/mol |
GV3101 (pSoup) Agrobacterium Electrocompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-217-5x50 | 5 x 50 μL | $ 199.00 | |
CC-217-10x50 | 10 x 50 μL | $ 359.00 | |
CC-217-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s GV3101 Agrobacterium electrocompetent cells are a powerful tool for high efficiency transformation of plants. The GV3101 strain is optimized for cDNA and gDNA library construction, and possesses resistance to rifampicin, gentamicin, and tetracycline. The T-DNA region of the GV3101 Ti plasmid has been deleted, but restoration of the binary vector T-region can fully enable T-DNA binary system function.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101 (pSoup)
Transformation efficiency: ≥8 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup-P19) Agrobacterium Electrocompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-227-5x50 | 5 x 50 μL | $ 199.00 | |
CC-227-10x50 | 10 x 50 μL | $ 359.00 | |
CC-227-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s GV3101 electrocompetent Agrobacterium cells are a highly efficient tool for genetic transformation of dicotyledonous and monocotyledonous plant species, such as Arabidopsis thaliana, tobacco, potato, and corn. These cells carry the nopaline-type Ti plasmid pMP90 (pTiC58DT-DNA) that contains the vir genes required for the transfer and integration of T-DNA into the plant genome. Additionally, the cells are resistant to rifampicin, gentamicin, and tetracycline, which allows for selection of transformed cells.
The pSoup plasmid carried by the cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes. These combined properties make GV3101 electrocompetent Agrobacterium cells an ideal choice for cDNA or gDNA library construction and the generation of transgenic plants with desirable traits.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101 (pSoup-P19)
Transformation efficiency: ≥1 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Electrocompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-207-5x50 | 5 x 50 μL | $ 199.00 | |
CC-207-10x50 | 10 x 50 μL | $ 359.00 | |
CC-207-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥2 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
HB101 Chemically Competent E. coli Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-150-5x50 | 5 x 50 µl | $ 59.00 | |
CC-150-10x50 | 10 x 50 µl | $ 105.00 |
Description
GoldBio’s HB101 chemically competent E. coli cells allow you to obtain high transformation efficiency in applications such cloning and sub-cloning. Our E. coli HB101 proceeds from the K12 x B hybrid strain. HB101 strain also contains the recA13 mutation useful in the insert stability and minimization of recombination. E. coli HB101 also has the hsdS20(rB-mB-) genotype that prevents the cleavage of cloned DNA by endogenous restriction enzymes.
Product Specifications
Competent cell type: Chemically competent
Derivative of: HB101
Species: E. coli
Transformation efficiency: ≥7 x 106 cfu/µg pUC19 DNA
Blue/white screening: No
Hygromycin B EZ Pak™ for 50 mg/mL Solution
Catalog ID | Size | Pricing | |
---|---|---|---|
H-270-EZ25 | 25 mL | $ 169.00 | |
H-270-EZ50 | 50 mL | $ 269.00 | |
H-270-EZ100 | 100 mL | $ 405.00 |
Description
The Hygromycin B EZ Pak™ is the fastest and easiest way to make a set amount of sterile hygromycin B solution. The kit includes pre-weighed hygromycin B powder, a sterile filter and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio hygromycin B and the sterile solution is ready for tissue culture, bacterial media or any other appropriate use.
Product Specifications
Catalog ID | H-270-EZ |
---|---|
CAS # | 31282-04-9 |
MW | 527.52 g/mol |
UN Number | 3462 |
Item Class | 6.1 |
Group Number | I |
IFN-gamma, Equine
Catalog ID | Size | Pricing | |
---|---|---|---|
1060-06-100 | 100 μg | $ 365.00 |
Description
Interferon gamma is the only type II interferon and binds to the heterodimeric IFNGR that consists of two chains, IFNGR1 and IFNGR2. This interaction activates the JAK-STAT pathway. IFNG is produced primarily by T-lymphocytes and natural killer (NK) cells. It is critical for innate and adaptive immunity against viral and intracellular bacterial infections. This is due partly to its ability to inhibit viral replication directly, but also due to its immunostimulatory and immunomodulatory effects. IFNG induces the production of cytokines and upregulates the expression of class I and II MHC antigens, Fc receptors, and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching, and potentiates the secretion of immunoglobulins by B cells. Additionally, IFNG augments TH1 cell expansion and may be required for TH1 cell differentiation.
Product Specifications
Catalog ID | 1060-06 |
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MW | ~16.7 kDa |
Storage/Handling | Store desiccated at -20°C. |
IL1B (IL-1 beta), Equine
Catalog ID | Size | Pricing | |
---|---|---|---|
1010-01B-10 | 10 μg | $ 165.00 | |
1010-01B-100 | 100 μg | $ 1,315.00 | |
1010-01B-500 | 500 μg | $ 2,535.00 |
Description
Interleukin-1 beta (IL1B) is a nonsecreted pro-inflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells and endothelial cells. It possesses metabolic, physiological and hematopoietic activities, and plays one of the central roles in the regulation of immune responses. Both IL1A and IL1B bind to the same receptor and have similar but not identical biological properties.
Product Specifications
Catalog ID | 1010-01B |
---|---|
MW | ~17.3 kDa |
Storage/Handling | Store desiccated at -20°C. |
IL1RN (IL-1RA), Equine
Catalog ID | Size | Pricing | |
---|---|---|---|
1010-01C-5 | 5 μg | $ 115.00 |
Description
IL1RN, initially called the IL1 inhibitor, is encoded by the IL1RN gene which is a member of the interleukin 1 cytokine family. IL1RN is secreted by various types of cells, including immune cells, epithelial cells and adipocytes. IL1RN functions to inhibit the activity of interleukin-1 by binding to the IL1R1 receptor, which prevents it from associating with the co-receptor IL1RAP. IL1RN is also used in the treatment of rheumatoid arthritis, an autoimmune disease in which IL1 plays a key role. The Equus caballus IL1RN is a single nonglycosylated polypeptide chain that has been shown to block the inflammatory responses induced by IL1 both in vitro and in vivo. The protein shows 26% amino acid homology to IL1β and 19% homology to IL1α.
Product Specifications
Catalog ID | 1010-01C |
---|---|
MW | ~17.4 kDa |
Storage/Handling | Store desiccated at -20°C. |
IL2 (Cys141Ser), Equine
Catalog ID | Size | Pricing | |
---|---|---|---|
1010-02A-10 | 10 μg | $ 165.00 | |
1010-02A-100 | 100 μg | $ 905.00 | |
1010-02A-500 | 500 μg | $ 1,849.00 |
Description
IL2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T-cells, γδ T-cells, B-cells, dendritic cells, and eosinophils. The receptor for IL2 consists of three subunits that are present on the cell surface in varying preformed complexes. The 55 kDa IL2 Rα is specific for IL2 and binds with low affinity. The 75 kDa IL2 Rβ, which is also a component of the IL15 receptor, binds IL2 with intermediate affinity. The 64 kDa common gamma chain γc/IL2 Rγ, which is shared with the receptors for IL4, IL7, IL9, IL15 and IL21, does not interact independently with IL2. Upon ligand binding, signal transduction is performed by both IL2 Rβ and γc. It drives resting T-cells to proliferate and induces IL2 and IL2 Rα synthesis. It contributes to T-cell homeostasis by promoting the Fas-induced death of naive CD4+ T-cells but not activated CD4+ memory lymphocytes. IL2 plays a central role in the expansion and maintenance of regulatory T-cells, although it inhibits the development of Th17 polarized cells. This recombinant IL2 has been modified by substituting a serine for cysteine at position 141 of the original sequence.
Product Specifications
Catalog ID | 1010-02A |
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MW | ~14.9 kDa |
Storage/Handling | Store desiccated at -20°C. |
IPTG EZ Pak™ for 1 M Solution
Catalog ID | Size | Pricing | |
---|---|---|---|
I2481-EZ10 | 10 mL | $ 75.00 | |
I2481-EZ50 | 50 mL | $ 115.00 | |
I2481-EZ100 | 100 mL | $ 185.00 |
Description
The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.
Product Specifications
Catalog ID | I2481-EZ |
---|---|
CAS # | 367-93-1 |
MW | 238.30 g/mol |
JM109 Chemically Competent E. coli Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-114-5x50 | 5 x 50 µl | $ 59.00 | |
CC-114-10x50 | 10 x 50 µl | $ 105.00 |
Description
GoldBio’s JM109 Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, library construction, cloning, and subcloning. JM109 is a K strain bacterium that carries multiple features including the lacIqZΔM15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol as well as the hsdR17 genotype which prevents endogenous endonucleases from cleaving DNA.
Product Specifications
Competent cell type: Chemically competent
Derivative of: JM109
Species: E. coli
Format: Tubes
Transformation efficiency: ≥6 x 105 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
Catalog ID | CC-114 |
---|---|
Storage/Handling | Store Competent Cells at -80°C. |
Kanamycin Monosulfate EZ Pak™ for 50 mg/mL Solution
Catalog ID | Size | Pricing | |
---|---|---|---|
K-120-EZ25 | 25 mL | $ 65.00 | |
K-120-EZ50 | 50 mL | $ 89.00 | |
K-120-EZ100 | 100 mL | $ 125.00 |
Description
The Kanamycin Monosulfate EZ Pak™ is the fastest and easiest way to make a set amount of sterile kanamycin monosulfate solution. The kit includes pre-weighed kanamycin monosulfate powder, a sterile filter and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio kanamycin monosulfate and the sterile solution is ready for tissue culture, bacterial media, or any number of uses.
Product Specifications
Catalog ID | K-120-EZ |
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CAS # | 25389-94-0 |
MW | 582.58 g/mol |
meso-Erythritol
Catalog ID | Size | Pricing | |
---|---|---|---|
E-050-1 | 1 kg | $ 83.00 | |
E-050-5 | 5 kg | $ 273.00 | |
E-050-25 | 25 kg | $ 1,030.00 |
Description
meso-Erythritol is a compound used in cell culture to differentiate bacteria based on carbohydrate fermentation ability. Erythritol may be a ligand for the T1R3 receptor and is a precursor to L-erythrulose. It is useful in supporting the carbon source growth requirement of Gluconobacter bacteria. It has also been used as a reference material in purification and analysis procedures developed to assay fermentation broths.
Product Specifications
Catalog ID | E-050 |
---|---|
CAS # | 149-32-6 |
MW | 122.12 g/mol |
RR1 Chemically Competent E. coli Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-113-5x50 | 5 x 50 µl | $ 59.00 | |
CC-113-10x50 | 10 x 50 µl | $ 105.00 |
Description
GoldBio’s RR1 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli RR1 is a recA+ derivative of the HB101 strain and can be more useful than HB101 if a recA+ background is required.
Product Specifications
Competent cell type: Chemically competent
Derivative of: RR1
Species: E. coli
Format: Tubes
Transformation efficiency: ≥3 x 107 cfu/µg pUC19 DNA
Blue/white screening: No
Product Specifications
Catalog ID | CC-113 |
---|---|
Storage/Handling | Store Competent Cells at -80°C. |
Spectinomycin Dihydrochloride Pentahydrate EZ Pak™ for 100 mg/mL Solution
Catalog ID | Size | Pricing | |
---|---|---|---|
S-140-EZ25 | 25 mL | $ 59.00 | |
S-140-EZ50 | 50 mL | $ 85.00 | |
S-140-EZ100 | 100 mL | $ 115.00 |
Description
The Spectinomycin Dihydrochloride Pentahydrate EZ Pak™ is the fastest and easiest way to make a set amount of sterile spectimomycin dihydrochloride solution. The kit includes pre-weighed spectimomycin dihydrochloride powder, a sterile filter and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio spectimomycin dihydrochloride and the sterile solution is ready for tissue culture, bacterial media or any number of uses.
Product Specifications
Catalog ID | S-140-EZ |
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CAS # | 22189-32-8 |
MW | 495.35 g/mol |
Streptomycin Sulfate EZ Pak™ for 100 mg/mL Solution
Catalog ID | Size | Pricing | |
---|---|---|---|
S-150-EZ10 | 10 mL | $ 49.00 | |
S-150-EZ25 | 25 mL | $ 79.00 | |
S-150-EZ50 | 50 mL | $ 115.00 | |
S-150-EZ100 | 100 mL | $ 155.00 |
Description
The Streptomycin Sulfate EZ Pak™ is the fastest and easiest way to make a set amount of sterile streptomycin sulfate solution. The kit includes pre-weighed streptomycin sulfate powder, a sterile filter and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high-quality GoldBio streptomycin sulfate, and the sterile solution is ready for tissue culture, bacterial media or any number of uses.
Product Specifications
Catalog ID | S-150-EZ |
---|---|
CAS # | 3810-74-0 |
MW | 1457.40 g/mol |
TG1 Phage Display Electrocompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-205-5x100 | 5 x 100 μL | $ 188.00 | |
CC-205-10x100 | 10 x 100 μL | $ 326.00 | |
CC-205-15x100 | 15 x 100 μL | $ 476.00 |
Description
GoldBio’s TG1 Phage Display Electrocompetent E. coli cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.TG1 Phage Display cells have ≥1 x 109 cfu/µg efficiency with electroporation and have Amber suppressor strain (supE).
Product Specifications
Competent cell type: ElectroCompetent
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes
Timentin™ Ticarcillin/Clavulanate (15/1)EZ Pak™ for 100 mg/mL Solution
Catalog ID | Size | Pricing | |
---|---|---|---|
T-104-EZ10 | 10 mL | $ 79.00 | |
T-104-EZ50 | 50 mL | $ 155.00 | |
T-104-EZ100 | 100 mL | $ 209.00 |
Description
The Timentin™ EZ Pak™ is the fastest and easiest way to make a set amount of sterile Timentin™ solution. The kit includes pre weighed Timentin™ powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio Timentin™ and the sterile solution is ready for tissue culture, bacterial media, or any number of uses.
Product Specifications
Catalog ID | T-104-EZ |
---|---|
CAS # | 4697-14-7; 61177-45-5 |
MW | 665.64 g/mol |