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How do I Get my Protein Out of Inclusion Bodies?

Have you ever had a problem where you had to try many different potential solutions before you final...

Understanding Salting In and Salting Out: Salt as a Protein Purification Tool

Salt is a major component in protein purification buffers, and optimizing this important parameter c...

How to Use Magnetic Agarose Beads to Purify Antibodies: A Step-by-Step Protocol

This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, o...

Showing articles tagged with “pcr”

There are several experimental techniques to detect and quantify nucleic acids in your sample. These...

When performing a qPCR or RT-qPCR, a PCR probe is usually used to detect the target sequence using f...

PCR probes are DNA or RNA sequences labeled with a reporter molecule. The reporter is a molecule tha...

You have just finished your real-time quantitative reverse transcription PCR or RT-qPCR experiment....

Although real-time quantitative reverse transcription PCR, or RT-qPCR, is a powerful tool for many d...

Real-time quantitative reverse transcription PCR or qRT-PCR is a technique used in molecular biology...

La clonación molecular nos permite estudiar genes, modificar genes, reintroducir los genes modificad...

During tissue culture work, finding unwanted contaminated culture flasks can create many issues, inc...

Cresol red is a triarylmethane dye and it can be used as an alternative loading dye for gel electrop...