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How do I Get my Protein Out of Inclusion Bodies?
Have you ever had a problem where you had to try many different potential solutions before you final...
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Understanding Salting In and Salting Out: Salt as a Protein Purification Tool
Salt is a major component in protein purification buffers, and optimizing this important parameter c...
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How to Use Magnetic Agarose Beads to Purify Antibodies: A Step-by-Step Protocol
This article provides a step-by-step protocol for purifying antibodies using Protein A, Protein G, o...
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Showing articles tagged with “buffers”

All About the Composition of Protein Purification Buffers and Why It Matters
Protein purification buffers are complex and can contain reducing agents, detergents, salts, imidazo...

3 Functions of Buffer Solutions in Bioscience Experiments
Buffers are mainly used three ways. First maintaining pH within a range. Second, buffers supply ions...

Buffer Preparation: Tips and Tricks
You’re preparing buffers in the lab, and maybe you’ve been following a protocol for the longest time...

A Quick Guide to Buffers and pH
pH is the measure of acidity or basicity of a solution. Tightly maintaining the pH to the desired ra...

The Difference Between Tris (Tris Base) vs. Tris HCl
Tris (tris base) vs. tris HCl – what’s the difference? The quick answer is that tris is a basic buff...

How to Pronounce Biological Buffers by GoldBio
It is an inherently human trait to look for patterns in objects or things that we can personalize or...