DNA Gel Electrophoresis Introduction & Overview
Electrophoresis is a technique where similarly charged molecules in a mixture flow in the same direc...
GelRed™ is an ultra-sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed™ Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.
6X GelRed™ Prestain Loading Buffer with Orange Tracking Dye contains an orange electrophoresis tracking dye that run at approximately 50 bp in a 1% agarose gel.
GelRed™ and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed™ is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed™ is compatible with downstream applications such as sequencing and cloning. GelRed™ is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.
A series of safety tests have confirmed that GelRed™ is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed™ can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal.
|Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic
|Passed environmental safety tests for direct disposal down the drain or in regular trash
|Higher sensitivity than EtBr and SYBR® Safe
|Available in water, stable at room temperature for long-term storage and microwavable
|Very simple procedures for precast or post-electrophoresis gel
|Replaces EtBr with no optical setting change
|Compatible with gel purification, restriction digest, sequencing and cloning
Storage/Handling: Store at 4°C. Protect from light.
Product may be shipped at ambient temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.
GelRed and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. SYBR is trademark of Molecular Probes, Inc.
|Store at 4°C. Protect from light.
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