4-Methylumbelliferyl beta-D-glucuronide (MUG) is a fluorogenic substrate for beta-glucuronidase (GUS). Upon cleavage, MUG yields blue fluorescence seen under UV light.
This substrate is commonly used for the detection of Escherichia coli through its ability to detect activity of beta-glucuronidase, which is encoded by uidA. GUS activity is tested in minimal medium o-nitrophenyl-beta-D-galactopyranoside (MMO)-MUG.
It is ideal for plant molecular study as a way to examine gene expression under various promoters. And while it works well for measuring activity in plant lysate and leaf disks, it can also work in whole plant assays.
4-Methylumbelliferyl beta-D-glucuronide dihydrate
MOLECULAR BIOLOGY GRADE
Formula: C16H16O9 • 2H2O
MW: 388.32 g/mol (anhydrous: 352.29 g/mol)
Storage/Handling: Store desiccated at -20°C.
PubChem CID: 16218413
|CAS #||6160-80-1 or 881005-91-0|
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store desiccated at -20°C.|
Gus Gene Assay in Transformed Plant Tissue
Procedures for GUS gene expression, fluorimetric and fluorigenic assays by Dr. Paul Bottino, Plant Molecular Genetics (retired), University of Maryland.
MUG Agar Preparation Protocol
MUG Agar Repipe for the identification and isolation of E. coli strains.
Fluorometric Assay for Protease Inhibitors in Plant Tissues utilizing MUG Protocol
A quick fluorometric assay for protease inhibitors using MUG by R. Jefferson et al. EMBO Journal, 1987.