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Competent Cells and Transformation
GoldBio offers an extensive collection of efficient chemically competent and electrocompetent cells with high transformation efficiencies to match your specific downstream applications: general cloning, protein expression, library construction, and many more.
- GoldBio's wide range of chemically competent and electrocompetent cells are optimized to provide lot-to-lot consistency and up to 10X higher transformation efficiency.
- Our universal recovery medium (SOC) formulation accompanies ALL of GoldBio's competent cells.
- We carry multiple Endonuclease I-deficient (endA1) competent bacterial cell strains to suit your cloning needs and ensure your transformations result in high quality and yield of plasmid DNA.
- Choose from E. coli strains with different selectable markers and screening options including the classic blue/white selection.
- GoldBio cells are ideal for distinct downstream applications from routine cloning/subcloning and protein expression to the more complex library construction, and cloning of difficult targets.
- Our electrocompetent Agrobacterium cells contain an efficient T-DNA binary system for genetic material transfer into the host plant cells.
CHOOSE THE BEST COMPETENT CELLS FOR YOUR APPLICATION!
Our DH5-alpha, BL21, BL21(DE3), DH10B, DH10B-Pro, DL39(DE3), and Agrobacterium cells are available in multiple formats to provide you with flexibility when planning your experiments. GoldBio also provides easy-to-follow protocols, FAQs, protocol videos and selection guides to ensure success of your transformations!
Use GoldBio's Competent Cell Guide to Select the Right Competent Cells for your Assay
Includes transformation efficiencies, genotype, strain information, screening methods and applicatio...
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BROWSE OUR TRANSFORMATION AND COMPETENT CELL RESOURCES
Your time in the lab is valuable! In this section you will find GoldBio's transformation tools designed to help support and simplify your competent cell transformations. You can access specific chemical transformation or electroporation protocols for each of our competent cell lines, antibiotic information, colony screening protocols, instructional blog articles, detailed guides containing competent cells characteristics and downstream applications, FAQs, electroporation troubleshooting tips and video protocols.
Electroporation and Electrocompetent Cells FAQs:
What is electroporation?
Electroporation is a technique commonly used in transformation assays to introduce DNA into an electrocompetent cell. During electroporation a transient current or electrical pulse is applied to cells that have been suspended in a solution. The current momentarily increases the permeability of the cell's membrane, creating holes (a pathway) that allow DNA (plasmids) to pass through and enter the cell. Generally, electroporation can be more effective than chemical transformation.
When should I use electrocompetent cells?
Electrocompetent cells have a higher transformation efficiency than chemically competent cells. The use of electrocompetent cells facilitate the transformation of large plasmids, including BACs (Bacterial Artificial Chromosomes), that might otherwise be difficult to transform using chemical transformation.
How do I prevent arcing during electroporation?
Arcing during electroporation can be avoided by making sure there are no air bubbles in the cell/DNA solution prior to electroporation. Also, desalt your DNA since the presence of conductive ions in the solution will cause arcing and electroporation failure. In addition, ensure that your equipment, including the cuvette is properly cleaned and in good condition.
Showing 21-22 of 22 items.
LBA4404 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-220-5x50 | 5 x 50 μL | $ 199.00 | |
| CC-220-10x50 | 10 x 50 μL | $ 359.00 | |
| CC-220-15x50 | 15 x 50 μL | $ 485.00 |
Description
GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
RR1 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-113-5x50 | 5 x 50 µl | $ 59.00 | |
| CC-113-10x50 | 10 x 50 µl | $ 105.00 |
Description
GoldBio’s RR1 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli RR1 is a recA+ derivative of the HB101 strain and can be more useful than HB101 if a recA+ background is required.
Product Specifications
Competent cell type: Chemically competent
Derivative of: RR1
Species: E. coli
Format: Tubes
Transformation efficiency: ≥3 x 107 cfu/µg pUC19 DNA
Blue/white screening: No
Product Specifications
| Catalog ID | CC-113 |
|---|---|
| Storage/Handling | Store Competent Cells at -80°C. |
