Competent Cells and Transformation

GoldBio offers an extensive collection of efficient chemically competent and electrocompetent cells with high transformation efficiencies to match your specific downstream applications: general cloning, protein expression, library construction, and many more.

  • GoldBio's wide range of chemically competent and electrocompetent cells are optimized to provide lot-to-lot consistency and up to 10X higher transformation efficiency.
  • Our enhanced recovery medium (SOC) formulation accompanies all of GoldBio's competent cells.
  • We carry multiple Endonuclease I-deficient (endA1) competent bacterial cell strains to suit your cloning needs and ensure your transformations result in high quality and yield of plasmid DNA.
  • Choose from E. coli strains with different selectable markers and screening options including the classic blue/white selection.
  • GoldBio cells are ideal for distinct downstream applications from routine cloning/subcloning and protein expression to the more complex library construction, and cloning of difficult targets.
  • Our electrocompetent Agrobacterium cells contain an efficient T-DNA binary system for genetic material transfer into the host plant cells.
  • GoldBio's amber suppressor (SupE) and non-amber suppressor (MC1061F\’) strains are ideal for phage display library screening.

CHOOSE THE BEST COMPETENT CELLS FOR YOUR APPLICATION!

Our DH5-alpha, BL21, BL21(DE3), DH10B, DH10B-Pro, DL39(DE3), Agrobacterium, L. lactis, SS320 and TG1 Phage Display cells are available in multiple formats to provide you with flexibility when planning your experiments. GoldBio also provides easy-to-follow protocols, FAQs, protocol videos and selection guides to ensure success of your transformations!

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GoldBio Competent Cells Consistently Exhibit Higher Transformation Efficiencies

DH10B chemically competent E. coli cells and DH10B Electrocompetent E. coli cells exhibit higher transformation efficiency than competitors. Each transformation with DH10B chemically competent E. coli cells was performed using 1 ml of final volume including recovery media, plating 50 μl after a 10 fold-dilution (left). For electrocompetent cell comparison, DH10B Electrocompetent E. coli cells were electroporated using a 1 ml of final volume including recovery media, plating 50 μl after 100-fold dilution (right).


BROWSE OUR TRANSFORMATION AND COMPETENT CELL RESOURCES

Your time in the lab is valuable! In this section you will find GoldBio's transformation tools designed to help support and simplify your competent cell transformations. You can access specific chemical transformation or electroporation protocols for each of our competent cell lines, antibiotic information, colony screening protocols, instructional blog articles, detailed guides containing competent cells characteristics and downstream applications, FAQs, electroporation troubleshooting tips and video protocols.

Electroporation and Electrocompetent Cells FAQs:

What is electroporation?

Electroporation is a technique commonly used in transformation assays to introduce DNA into an electrocompetent cell. During electroporation a transient current or electrical pulse is applied to cells that have been suspended in a solution. The current momentarily increases the permeability of the cell's membrane, creating holes (a pathway) that allow DNA (plasmids) to pass through and enter the cell. Generally, electroporation can be more effective than chemical transformation.

When should I use electrocompetent cells?

Electrocompetent cells have a higher transformation efficiency than chemically competent cells. The use of electrocompetent cells facilitate the transformation of large plasmids, including BACs (Bacterial Artificial Chromosomes), that might otherwise be difficult to transform using chemical transformation.

How do I prevent arcing during electroporation?

Arcing during electroporation can be avoided by making sure there are no air bubbles in the cell/DNA solution prior to electroporation. Also, desalt your DNA since the presence of conductive ions in the solution will cause arcing and electroporation failure. In addition, ensure that your equipment, including the cuvette is properly cleaned and in good condition.

Showing 21-23 of 23 items.

LBA4404 Agrobacterium Chemically Competent Cells

Catalog ID Size Pricing
CC-107-5x50 5 x 50 μL $ 227.00   $ 193.00
CC-107-10x50 10 x 50 μL $ 408.00
CC-107-15x50 15 x 50 μL $ 599.00

Description

GoldBio’s LBA4404 Agrobacterium chemically competent cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants.LBA4404 contains a rifampicin resistance gene (rif) and also has streptomycin resistance.LBA4404 strain also contains an octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.

Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: LBA4404
Format: Tubes
Transformation efficiency: ≥1 x 105 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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CC-107-5x50 5 x 50 μL $ 227.00
$ 193.00
CC-107-10x50 10 x 50 μL $ 408.00
CC-107-15x50 15 x 50 μL $ 599.00

LBA4404 Agrobacterium ElectroCompetent Cells

Catalog ID Size Pricing
CC-220-5x50 5 x 50 μL $ 233.00   $ 199.00
CC-220-10x50 10 x 50 μL $ 430.00
CC-220-15x50 15 x 50 μL $ 617.00

Description

GoldBio’s LBA4404 Agrobacterium ElectroCompetent cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction. The LBA4404 strain is useful for transgenic operations of tomatoes, tobacco and other plants.LBA4404 contains a rifampicin resistance gene (rif) and also has streptomycin resistance.LBA4404 strain also contains an octoprine-type Ti plasmid pAL4404 without self-transport function, which contains the vir gene.

Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: LBA4404
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No

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,
CC-220-5x50 5 x 50 μL $ 233.00
$ 199.00
CC-220-10x50 10 x 50 μL $ 430.00
CC-220-15x50 15 x 50 μL $ 617.00

TG1 Phage Display Electrocompetent Cells

Catalog ID Size Pricing
CC-205-5x100 5 x 100 μL $ 188.00
CC-205-10x100 10 x 100 μL $ 326.00
CC-205-15x100 15 x 100 μL $ 476.00

Description

GoldBio’s TG1 Phage Display Electrocompetent E. coli cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.TG1 Phage Display cells have ≥1 x 107 cfu/µg efficiency with electroporation and have Amber suppressor strain (supE).

Product Specifications
Competent cell type: ElectroCompetent
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes

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,
CC-205-5x100 5 x 100 μL $ 188.00
CC-205-10x100 10 x 100 μL $ 326.00
CC-205-15x100 15 x 100 μL $ 476.00

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