EvaGreen® Dye, 20x in Water
Product Description
EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making the dye readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen® dye is nonmutagenic and noncytotoxic by being completely impermeable to cell membranes, making it a great alternative to SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et al. (2001). Mutation Research, 492(1), 91-97).
The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.
Features
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Exhibits much less PCR inhibition than SYBR® Green I via a smart “release-on-demand” DNA-binding technology
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Low PCR inhibition of the dye permits a higher dye concentration to be used for much greater qPCR and melt curve analysis signals
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Nonmutagenic and noncytotoxic by standard Ames test; completely impermeable to cell membranes |
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Minimal interference to PCR makes it possible to significantly shorten the chain extension time
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No dye migration from amplicon to amplicon when used at the recommended concentration
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No detectable dye decomposition in PCR buffer at 95-100°C for 48 hours; highly stable under either alkaline or acidic condition; withstands repeated freeze-thaw cycles
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Compatible with all major brand qPCR instruments and enzyme systems |
Storage/Handling: Store at 4°C. Protect from light.
Product may be shipped at ambient temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.
EvaGreen® dye and applications are covered under US patent nos. 7,803,943 and 7,776,567 and pending international patents.
Product Specifications
| Catalog ID | E-670 |
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| Storage/Handling | Store at 4°C. Protect from light. |
