Amikacin hydrate is a broad-spectrum aminoglycoside antibiotic derived from kanamycin A. It is effective against gram-negative bacteria such as Pseudomonas aeruginosa and a variety of enteric species. It is also effective against Mycobacterium tuberculosis. Amikacin hydrate is freely soluble in aqueous solution.
Aminoglycoside antibiotics are composed of amino groups attached to glycosides. They bind the 30s ribosomal subunit, causing misreading of the mRNA sequence and inhibition of translocation. Consequently, protein synthesis is inhibited.
Samples of microbes grown in the presence of a 30 μg amikacin disc with a zone of inhibition of <14 mm in diameter are considered resistant. Intermediate resistance zones of inhibition are typically 15 mm-16 mm in diameter.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
MOLECULAR BIOLOGY GRADE
Formula: C22H43N5O13 ∙ XH2O
MW: 585.60 g/mol (anhydrous)
Storage/Handling: Store desiccated at 2-8°C.
PubChem Chemical ID: 37768
|1257517-67-1 / 37517-28-5 (anhydrous)
|MOLECULAR BIOLOGY GRADE
|Store desiccated at 4°C.