3 Key Differences Between AEBSF and PMSF Protease Inhibitors
AEBSF and PMSF are both irreversible protease inhibitors of serine proteases. With them both inhibit...
AEBSF is an irreversible serine protease inhibitor. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin, kallikrein and thrombin. It inhibits the active site of the enzyme through acylation. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25mM. Aqueous solutions are stable between pH 5-6; limited stability above pH 7.5.
Attention Canadian and Mexican customers: This product is a hazardous and can only ship via Air.
Store at -20°C. Protect from light.
Make stock solutions at 100mM. Aliquot and store at -20°C for up to 2 months.
AEBSF working concentrations are usually between 0.1-1.0mM and should be made daily.
Catalog ID | A-540 |
---|---|
Name(s) |
AEBSF·HCl; Pefabloc SC; 4-(2-Aminoethyl)-benzenesulfonylfluoride hydrochloride |
CAS # | 30827-99-7 |
Formula | C8H10FNO2S · HCl |
MW | 239.69 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
PubChem Chemical ID | 186136 |
Storage/Handling | Store at -20°C. Protect from light. |
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