6X Green Loading Dye

GoldBio's 6x Green DNA Loading Dye is a pre-mixed buffer for tracking DNA samples during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 4,000 bp and 50 bp on a standard 1% TAE agarose gel respectively (800 bp and 30 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Composition
0.03% Xylene cyanol FF
0.15% Orange G
10mM Tris-HCl (pH 8.0)
60% glycerol
60mM EDTA

Storage/Handling: Store at 4°C.