Thiazolyl Blue Tetrazolium Bromide (MTT)

Thiazolyl blue tetrazolium bromide (often called MTT) is a common dye used in Cell Proliferation assays or Cell Growth assays. MTT is converted to water-insoluble, dark blue MTT-formazan by mitochondrial dehydrogenases of living cells. The intensity of the color can then be measured colorimetrically at a wavelength of 570 nm.

Product Specifications:
Methylthiazolyldiphenyl-tetrazolium bromide,
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
MTT

Formula: C₁₈H₁₆BrN₅S

MW: 414.32 g/mol

Handling/Storage: Store dark at 4°C, protect from light/ air, keep dry.
Soluble in Water or PBS up to 5 mg/ml, and in Ethanol at 20 mg/ml.

PubChem Chemical ID: 64965