MOPS Free Acid, Ultra Pure
MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.
MOPS free acid can be mixed with MOPS sodium salt to attain desired pH. Alternatively, MOPS free acid can be titrated with sodium hydroxide to attain desired pH.
MOPS, Ultra Pure
MW: 209.26 g/mol
Storage/Handling: Store in a dry and well-ventilated place.
PubChem Chemical ID: 70807
|Storage/Handling||Store desiccated at room temperature.|
Protocol for RNA Gel and Northern Blotting - adapted from Wolf Lab, Sanford-Burnham Medical Research Institute.
10X MOPS Running Buffer Preparation protocol, pH 7.0 (200mM MOPS)
This ultimate buffer selection guide is a fantastic graphical display, showing common buffer pH ranges and their associated pKa. Buffers on this chart are also categorized by family. It's perfect to print out for your lab.
Refer to this printable buffer-pH chart for common buffers, associated pKa and pH range. Information on this chart is displayed in vibrant colors, and makes a great print-out for your lab.
A user guide for the background, experimental use and technical discussion of GoldBio biological buffers.
The Biological Buffer Selection Guide includes catalog number, pH range, pKa, applications, stock solution protocol availability and comments for the most common biological buffers.
Sample Certificate of Analysis - MOPS, Ultra Pure
COA for example only. Actual tests may differ.