EDTA Disodium, dihydrate

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Product Description

EDTA (Ethylenediaminetetraacetic acid) is a chelating agent, a general chemical and a sequestrant. In molecular biology applications, it is used to minimize metal ion contamination and prevent enzymatic activity.

EDTA disodium is routinely used in electrophoresis DNA and protein separation applications to chelate metal ions required for enzymatic activity that could potentially damage DNA and protein structure.

Product Specifications
EDTA Disodium, Dihydrate
Ethylenediaminetetraacetic acid

MOLECULAR BIOLOGY GRADE

Formula: C10H14N2Na2O8 ∙ 2H2O

MW: 372.24 g/mol

Storage/Handling: Store in a dry and well-ventilated place.

PubChem Chemical ID: 636371

Product Specifications

Catalog ID E-210
CAS # 6381-92-6
MW 372.24 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at room temperature.

Technical Documentation

P Protocol
O Other
C Certificate of Analysis
S Safety Data Sheet
P

BRET (Bioluminescence Resonance Energy Transfer) Method

High Throughput Bioluminescence Resonance Energy Transfer (BRET) Method using Rluc and Coelenterazine by SW Gersting, et al. (2012)
P

IPTG Induction and Extraction of Proteins Protocol

IPTG Induction & Extraction of Proteins - by Arur and Nayak. Schedl Lab, Wash U. St. Louis
P

Agarose Gel Preparation Protocol

Protocol for melting and pouring an agarose gel
P

TAE Stock Solution - 50X

50X TAE Stock Solution Buffer for gel electrophoresis.
P

TBE Stock Solution - 10X

10X TBE Stock Solution Buffer for gel electrophoresis.
P

TTE Stock Solution - 10X

10X Tris-TAPS-EDTA Stock Solution Buffer for gel electrophoresis
P

TE Buffer Stock Solution - 10X

10X TE Buffer for storage of nucleic acids such as DNA and RNA
P

EDTA Stock Solution

EDTA stock solution protocol and storage
P

Electrotransformation of Agrobacterium tumefaciens

Use of electroporation to modify Agrobacterium tumefaciens.

P

Radio Immunoprecipitation Assay (RIPA) Cell Lysate Preparation

Use of RIPA Buffer to prepare cell lysates for protein assays, protein purification and other downstream applications.

O

Guide: Commonly used Buffers

Guide: Commonly used Buffers contains name, pH range, applications, composition and other characteristics of buffers commonly used in the lab.

C

Sample COA - E-210

Sample Certificate of Analysis - EDTA Disodium
COA for example only. Actual tests may differ.
S

SDS (MSDS) - E-210

Safety Data Sheet - EDTA Disodium, dihydrate

Citations

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