Troubleshooting PCR Part Two: What to Do When You Are Not Getting Bands
PCR (Polymerase Chain Reaction), which is so common to the lab, can be very frustrating. We unders...
Dimethylsulfoxide (DMSO) is a very polar organic compound that is useful in organic chemistry for solubilizing organic and inorganic compounds. DMSO is also used as a cryoprotectant for cell media, preventing the formation of ice crystals, which would otherwise damage cells. DMSO can be used in PCR reactions to inhibit the self-complementation of DNA or PCR primers and increase amplification, especially for DNA sequences, which have GC rich sequences.
Product Specifications
Dimethylsulfoxide, ACS Grade
DMSO
Formula: C2H6SO
MW: 78.13 g/mol
Storage/Handling: Store desiccated at room temperature.
PubChem Chemical ID: 679
Note: DMSO ships as multiple 25 ml units to qualify for a small quantity exception to hazardous fees.
Catalog ID | D-360 |
---|---|
CAS # | 67-68-5 |
MW | 78.13 g/mol |
Storage/Handling | Store desiccated at room temperature. |
PCR (Polymerase Chain Reaction), which is so common to the lab, can be very frustrating. We unders...
When you’ve conducted the same reaction a dozen times and the prob...
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